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Showing: 501 - 520 of 2546 results

  2. pLVX-H3.3K27M-FLAG-BFP

    Plasmid
    #209434
    Purpose
    Lentiviral packagable plasmid expressing FLAGGED human histone mutant H3.3-K27M with BFP
    Depositor
    Maria Castro
    Insert
    H3.3-K27M
    Use
    Tags
    Expression
    Mammalian
    Mutation
    K27M
    Promoter
    Available since
    March 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    209434-plasmid-map-sequence-id-424833

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  5. SOX2-P2A-tagBFP-HDR

    Plasmid
    #163751
    Purpose
    HDR knock-in template for tagging the human endogenous SOX2 gene with a tagBFP fluorescent marker linked by a self-cleaving P2A peptide.
    Depositor
    Jennifer Mitchell
    Article
    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.
    Insert
    SOX2 (SOX2 Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    P2A-tagBFP
    Expression
    Mutation
    Does not contain start codon to avoid random inte…
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    163751-plasmid-map-sequence-id-326711

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  12. pIRES-H3.3-WT-FLAG-BFP

    Plasmid
    #206139
    Purpose
    Expression of a FLAG tagged human histone H3.3 gene with BFP
    Depositor
    Maria Castro
    Insert
    H3.3 (H3-3A Human)
    Use
    Tags
    FLAG-eBFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    206139-plasmid-map-sequence-id-405576

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  14. pAP215.rAAV.mU6-sgRNA.Handle.EF1a-mTagBFP2

    Plasmid
    #217635
    Purpose
    AAV backbone for sgRNA expression. Contains a Lox-flanked handle sequence for Cre-dependent PCR amplification of sgRNAs. Protospacer is cloned between BstXI and BlpI.
    Depositor
    Martin Kampmann
    Insert
    mU6-sgRNA, Handle sequence, EF1a-mTagBFP2
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    EF1alpha and mU6
    Available since
    Oct. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    217635-plasmid-map-sequence-id-449624

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  17. pN3-mTagBFP-pHluorin-CAAX

    Plasmid
    #178345
    Purpose
    Express a pH insensitive blue fluorencent protein mTagBFP fused to the N-terminal of a membrane targeted pH sensitive green fluorescent protein pHluorin-CAAX under CMV promoter
    Depositor
    Yulong Li
    Article
    Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1
    Insert
    mTagBFP-pHluorin-CAAX
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    178345-plasmid-map-sequence-id-354972

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  19. iC13 PB-TO-oNgn2-BFP-puro

    Plasmid
    #204733
    Purpose
    Improved Dox-inducible neurogenin-2; Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase.
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Insert
    Optimized neurogenin-2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    204733-plasmid-map-sequence-id-403381

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Showing: 501 - 520 of 2546 results