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Showing: 501 - 520 of 2236 results

  5. pToxAmp21R

    Plasmid
    #218287
    Purpose
    A complete plasmid for ToxAmp (Toxin-antitoxin-driven gene amplification) for the expression of AeBlue
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    HO(-955, -789)-LoxP>pKlLEU2>KlLEU2>PCRT1>RelB>tPDC1-pTDH3>AeBlue>tSYNth7-ARS712-HO(-731, -264)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218287-plasmid-map-sequence-id-433689

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  6. pToxAmp12

    Plasmid
    #218286
    Purpose
    Multi-copy integration of heterologous genes (AeBlue and RelB) through co-transformation with ToxAmp (toxin-antitoxin-driven gene amplification) modules
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    HO(-253, -1)-pCRT1>RelB>tPDC1-pTDH3>AeBlue>tsynth7-ARS712-HO(-731, -264)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218286-plasmid-map-sequence-id-433688

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  7. pToxAmp1

    Plasmid
    #218285
    Purpose
    Amplifying the gene copy number of heterologous genes (yEGFP and RelB) through ToxAmp (toxin-antitoxin-driven gene amplification) mechanism
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    HO(-253, -1)-pRPL8B>RelB>tPDC1-pTEF1>yEGFP>tURA3-ARS712-HO(-731, -264)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218285-plasmid-map-sequence-id-433687

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  9. OptoProfilin.S138E

    Plasmid
    #208287
    Purpose
    Expresses Cry2(1-531)-mCherry-Profilin.S138E
    Depositor
    Robert Hughes
    Article
    Mann et al ChemBioChem, 8 March 2024
    Insert
    Cry2 - mCherry - Profilin.S138E (Cry2, PRF1 Mustard Weed, Mouse)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Ser 138 (sometimes numbered 137) mutated to Glu
    Promoter
    CMV
    Available since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    208287-plasmid-map-sequence-id-410531

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  10. OptoProfilin.S138A

    Plasmid
    #208288
    Purpose
    Expresses Cry2(1-531)-mCherry-Profilin.S138A
    Depositor
    Robert Hughes
    Article
    Mann et al ChemBioChem, 8 March 2024
    Insert
    Cry2 - mCherry - Profilin (Cry2, PRF1 Mustard Weed, Mouse)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Ser 138 (sometimes numbered 137) mutated to Ala
    Promoter
    CMV
    Available since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    208288-plasmid-map-sequence-id-410535

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  14. iEscSnFR

    Plasmid
    #182807
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do
    Insert
    iEscSnFR
    Use
    Tags
    6xHis, HA and Myc
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    182807-plasmid-map-sequence-id-358170

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  15. iFluoxSnFR

    Plasmid
    #182808
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do
    Insert
    iFluoxSnFR
    Use
    Tags
    6xHis, HA and Myc
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    182808-plasmid-map-sequence-id-358173

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  16. pGEX-2T-CBP(1-287)

    Plasmid
    #182839
    Purpose
    CBP(1-287) was inserted into pGEX-2T with NdeI and NsiI sites. pGEX-2T-CBP(1-287) was used in the GST pull-down assay described in our paper (Tie et al. 2012 MCB, Tie et al 2014 Development)
    Depositor
    Peter Harte
    Article
    Tie et al Development. 2014 Mar;141(5):1129-39. do
    Insert
    CBP1-287
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    182839-plasmid-map-sequence-id-360063

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  20. pAAV-DYRK2-2A-mCherry

    Plasmid
    #118285
    Purpose
    Expresses mouse DYRK2 tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells.
    Depositor
    Vance Lemmon
    Article
    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5
    Insert
    dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2 (Dyrk2 Mouse)
    Use
    AAV
    Tags
    2A peptide and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV with beta global intron
    Available since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    118285-plasmid-map-sequence-id-229470

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Showing: 501 - 520 of 2236 results