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Plasmid#176651PurposeIt encodes an mVenus fused to a mutant p27K-, which lacks binding affinity to Cdk. This reporter helps to identify quiescent disseminated tumor cells (in G0 phase of cell cycle).DepositorInsertmVenus-p27K- (Cdkn1b Mouse)
UseLentiviralTagsmVenusExpressionMutationMutant K- (lacks affinity to Cdk)PromoterAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-P2A-BSD
Plasmid#174821PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-BSD markerDepositorInsertPEmax-P2A-BSD
UseTagsP2A-BSD, SV40 bpNLS, and c-Myc NLSExpressionMammalianMutationDetailed in manuscriptPromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tet-off TauRD (P301L/V337M)
Plasmid#188572PurposeDox-repressible expression of the Tau repeat domainDepositorInsertTau repeat domain (MAPT Human)
UseLentiviralTagsMycExpressionMammalianMutationP301L and V337MPromoterpTight TREAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-P2A-hMLH1dn
Plasmid#174827PurposeMammalian expression of SpCas9 prime editor 2 with P2A-human MLH1dn (codon optimized)DepositorInsertPE2-P2A-hMLH1dn
UseTagsSV40 bpNLSExpressionMammalianMutationDetailed in manuscript; deletion of residues 754-…PromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-Nm-dCas9-p300 Core
Plasmid#61365Purposeencodes N. meningiditis dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoterDepositorInsertNm-dCas9-p300 Core (EP300 Synthetic, Human, Neisseria meningiditis)
UseCRISPR and Synthetic BiologyTagsHA tag and NLSExpressionMammalianMutationD16A, D587A, H588A, N611A in Nm Cas9PromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-FLp300
Plasmid#61356Purposeencodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 Synthetic, Human, S. pyogenes)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterCMVAvailable sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-HAPLN1
Plasmid#200777PurposeExpresses full-length mouse HAPLN1 fused with cysteine-free GFP (cfGFP) under synapsin promoterDepositorInsertHyaluronan And Proteoglycan Link Protein 1 (Hapln1 Mouse)
UseAAVTagscfGFPExpressionMutationPromoterSynapsinAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-∆Hapln1
Plasmid#200778PurposeExpresses mouse HAPLN1 mutant lacking the lectican binding domain and fused with cysteine-free GFP (cfGFP) under synapsin promoterDepositorInsertHyaluronan And Proteoglycan Link Protein 1 (Hapln1 Mouse)
UseAAVTagscfGFPExpressionMutationdeleted lectican binding domain (aminoacids 40-15…PromoterSynapsinAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.0-Magneto2.0-p2A-mCherry
Plasmid#74308PurposeBicistronic expression of Magneto2.0 and mCherry in mammalian cellsDepositorUseTagsFlag and p2A-mCherryExpressionMammalianMutationTRPV4: delta 760–871PromoterCMVAvailable sinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR 223 VPS4B
Plasmid#169320PurposeGateway entry vector for human VPS4B ORFDepositorInsertVPS4B (VPS4B Human)
UseGateway entry vectorTagsExpressionMutationPromoterNAAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Neo-GFP/Foxa1
Plasmid#105506PurposeMSCV-driven retroviral Foxa1 expressionDepositorInsertFoxa1 (Foxa1 Mouse)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR8-Magneto2.0-p2A-mCherry
Plasmid#74334PurposeFor gateway cloning Magneto2.0-p2A-mCherry into destination vectorDepositorUseGateway vectorTagsFlag and p2A-mCherryExpressionMutationTRPV4: delta 760–871PromoternoneAvailable sinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR 223 VPS4A
Plasmid#169319PurposeGateway entry vector for human VPS4A ORFDepositorInsertVPS4A (VPS4A Human)
UseGateway entry vectorTagsExpressionMutationPromoterNAAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT7NT*-Bri2 113-231 R221E
Plasmid#138134PurposeExpresseds human Bri2 BRICHOS R221E mutant in E. coliDepositorInserthuman Bri2 BRICHOS R221E (ITM2B Human)
UseTagsNT* tag derived from spider silk proteinsExpressionBacterialMutationPromoterT7Available sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Neo-GFP/Flag-Gata5
Plasmid#105508PurposeMSCV-driven retroviral Gata5 expression (Flag-tagged)DepositorInsertGata5 (Gata5 Mouse)
UseRetroviralTagsFlagExpressionMutationPromoterAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-ARAP3(R307,8A)
Plasmid#39486DepositorInsertARAP3(R307,8A) (ARAP3 Human)
UseTagsEGFPExpressionMammalianMutationR307,8A = PH domain mutationPromoterAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
Myc-gamma2S/deltaIL
Plasmid#119730PurposeGABAA receptor expression (chimeric rat gamma2 short subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat delta subunit) Myc-tag near N-terminusDepositorUseTagscMyc epitope EQKLISEEDL inserted between fourth a…ExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-ARAP3(C504A)
Plasmid#39485DepositorInsertARAP3(C504A) (ARAP3 Human)
UseTagsEGFPExpressionMammalianMutationC504A = Arf GAP domain mutationPromoterAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Neo-GFP/V5-Foxa1
Plasmid#105507PurposeMSCV-driven retroviral Foxa1 expression (V5-tagged)DepositorInsertFoxa1 (Foxa1 Mouse)
UseRetroviralTagsV5ExpressionMutationPromoterAvailable sinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO shLuc/FLAG Metap2
Plasmid#209316PurposeLentiviral construct expressing FLAG Metap2 and shRNA against Luciferase (control shRNA)DepositorInsertMETAP2 (METAP2 Human, human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterPGKAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO shLYCHOS/LYCHOS WT-FLAG
Plasmid#209315PurposeLentiviral construct expressing shRNA against LYCHOS and LYCHOS WT-FLAG (resistant to shRNA)DepositorInsertLYCHOS (GPR155 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterPGKAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO shLYCHOS/FLAG Metap2
Plasmid#209314PurposeLentiviral construct expressing FLAG Metap2 and shRNA against LYCHOSDepositorInsertLYCHOS (GPR155 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterPGKAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFB-CG-LYCHOS FP>IA-HA-GFP
Plasmid#199688PurposeExpression of LYCHOS FP>IA mutant in insect cellsDepositorInsertLYCHOS (GPR155 Human)
UseTags10xHis, 6xHis, EGFP, HA, and HRV 3CExpressionInsectMutationF43 and P44 mutated to I and A respectivelyPromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(hCHAT)-PGKpuro2ABFP-W
Plasmid#163147PurposeLentiviral gRNA plasmid targeting human CHAT gene, co-expression of BFP tagDepositorInsertCHAT (CHAT Human)
UseCRISPR and LentiviralTagsBFPExpressionMammalianMutationPromoterU6Available sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
RG6-BAP1_E7-D192D
Plasmid#154024PurposeBichromatic Fluorescent Splicing Reporter Minigene for Mutant BAP1 Exon 7 c.576C>T, p.D192D (Synonymous Mutation)DepositorInsertBAP1 Exon 7 Fused to DsRed1 and eGFP (BAP1 Human)
UseSplicing reporterTagsDsRed1, FLAG, SV40 NLS, and eGFPExpressionMammalianMutationBAP1 c.576C>T, p.D192DPromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
RG6-BAP1_E11-G312G
Plasmid#154026PurposeBichromatic Fluorescent Splicing Reporter Minigene for Mutant BAP1 Exon 11 c.936T>G, p.G312G (Synonymous Mutation)DepositorInsertBAP1 Exon 11 Fused to DsRed1 and eGFP (BAP1 Human)
UseSplicing reporterTagsDsRed1, FLAG, SV40 NLS, and eGFPExpressionMammalianMutationBAP1 c.936T>G, p.G312GPromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tet-off Full length Tau (0N4R, P301L/V337M)
Plasmid#188573PurposeDox-repressible expression of the 0N4R Tau isoformDepositorInsert0N4R Tau (MAPT Human)
UseLentiviralTagsExpressionMammalianMutationP301L and V337MPromoterpTight TREAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core
Plasmid#61357Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (D1399Y)
Plasmid#61358Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; D1399Y mutation i…PromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 S HexaPro
Plasmid#154754PurposeMammalian expression vector for expression of the SARS-CoV-2 spike HexaPro variantDepositorInsertSpike (HexaPro variant) (S Severe acute respiratory syndrome coronavirus 2)
UseTags2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …ExpressionMammalianMutationEctodomain only (AAs 1-1208); 682-685 (furin site…PromoterAvailable sinceJuly 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDNA3-Flag-SPRTN-wt
Plasmid#110214PurposeExpression in mammalian cells of full-length wild-type SPRTN protein with a Flag-tag at N-terminus.DepositorInsertSPRTN (SPRTN Human)
UseTagsExpressionMammalianMutationP296L (see depositor comments below)PromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-DIO-Magneto2.0-sNRPpA
Plasmid#74307PurposeCre-dependent expression of Magneto2.0 in mammalian cellsDepositorUseAAV and Cre/LoxTagsFlagExpressionMammalianMutationTRPV4: delta 760-871PromoterCMV (after Cre recombination)Available sinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-DIO-TRPV4-p2A-ferritin-sNRPpA
Plasmid#74306PurposeBicistronic Cre-dependent expression of TRPV4 & ferritin in mammalian cellsDepositorUseAAV and Cre/LoxTagsFLAG and p2AExpressionMammalianMutationPromoterCMV (after cre recombination)Available sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2CG2-Neurog1-Magneto2.0-p2A-mCherry-pA
Plasmid#74302PurposeExpression of Magneto2.0-p2A-mCherry in Rohon-Beard sensory neurons of the zebrafishDepositorUseZebrafishTagsFLAG, cmcl2::EGFP, and p2A-mCherryExpressionMutationTRPV4: delta 760-871PromoterNeurog1Available sinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (Y1467F)
Plasmid#61362Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inavtivating mutation Y1467F) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; Y1467F mutation i…PromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET3a-SF-LMNC-TS
Plasmid#169374PurposeE. coli expression plasmid for human LMNA residues 31-542, corresponding to the shorter lamin C isoform lacking the unstructured head, with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.DepositorInsertLMNA residues 31-542 (LMNA Human)
UseTagsSplit-FlAsH Tag and TwinStrep TagExpressionBacterialMutationdeleted amino acid 1-30 (unstructured head domain…PromoterNative pET3a promotorAvailable sinceMay 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (1645/1646 RR/EE)
Plasmid#61359Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation 1645/1646 RR/EE) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; 1645/1646 RR/EE m…PromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA-dCas9-p300 Core (1396/1397 SY/WW)
Plasmid#61363Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation 1396/1397 SY/WW) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; 1396/1397 SY/WW m…PromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTL
Plasmid#203176PurposeCentromeric yeast expression vector, leucine selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_FLAG-Mkk7-JNK2-P2A-Hygro_Barcode
Plasmid#170228PurposeBarcoded lentiviral vector to express Mkk7-JNK2 fusion in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorUseLentiviralTagsFLAGExpressionMutationFusionPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (C1204R)
Plasmid#61361Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation C1204R) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; C1204R mutation i…PromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
BAP1-C91A (siRNA resistant)
Plasmid#108438PurposeStable expression of BAP1-C91A (catalytically inactive mutant) in mammalian cells, gene has an introduced siRNA-resistance against siBAP1.DepositorInsertBAP1 (BAP1 Human)
UseTagsExpressionMammalianMutationC91A: c.271T>G, c.272G>C; others: c.1641C&g…PromoterCMVAvailable sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
BAP1-wt (siRNA resistant)
Plasmid#108439PurposeStable expression of BAP1-wt in mammalian cells, gene has an introduced siRNA-resistance against siBAP1.DepositorInsertBAP1 (BAP1 Human)
UseTagsExpressionMammalianMutationc.1641C>T, c.1644T>A, c.1647T>C, c.1650C…PromoterCMVAvailable sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-Flag-SPRTN-E112A
Plasmid#110215PurposeExpression in mammalian cells of of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, and Flag-tag at N-terminus.DepositorInsertSPRTN (SPRTN Human)
UseTagsFlagExpressionMammalianMutationE112A, P296L (see depositor comments below)PromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Neo-GFP/V5-Foxa1-(P2A)-Flag-Gata5
Plasmid#105509PurposeMSCV-driven retroviral Foxa1 and Gata5 expression (P2A-linked, V5 and Flag-tagged each)DepositorUseRetroviralTagsFlag and V5ExpressionMutationPromoterAvailable sinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)
Plasmid#61364Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; H1415A/E1423A/Y14…PromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLCAR-CD19-28z
Plasmid#135991PurposeModular CD28-CD3z CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsExpressionMammalianMutationPromoterEF1a-shortAvailable sinceJune 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLCAR-CD19-CD3z
Plasmid#135993PurposeModular CD3z alone CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsExpressionMammalianMutationPromoterEF1a-shortAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -