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Plasmid#81053Purposebacterial expression of the catalytic domain of murine TET1DepositorInsertTET1 (Tet1 Mouse)
UseTags6HISExpressionBacterialMutationcatalytic domain amino acid 1367-2057PromoterT7Available sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV_eA3A T31A-dCas9
Plasmid#163537PurposeMammalian CG-to-GC base editingDepositorInserteA3A T31A-dCas9
UseCRISPRTagsExpressionMammalianMutationdCas9 (D10A, H840A)eA3A T31APromoterAvailable sinceDec. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A mKate2-hu_ncOGT WT
Plasmid#154282PurposeEntry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; WT)DepositorInsertO-GlcNAc Transferase (OGT Human)
UseGateway entry vectorTags2x FLAG and mKate2ExpressionMutationWT OGTPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMP1337
Plasmid#119270Purposefor cloning new sgRNAsDepositorInsertdcas9
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5 beta4
Plasmid#16037DepositorInsertbeta4 (ITGB4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-dSaCas9-2xNLS
Plasmid#107319PurposeExpresses SpCas9 fused to dSaCas9 in mammalian cellsDepositorInsertSpCas9-SaCas9 fusion
UseCRISPRTagsNLSExpressionMammalianMutationSpCas9 is fused to nuclease dead SaCas9 (D10A, N5…PromoterCMV IE94Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A hu_ncOGT WT (open N-term)
Plasmid#155197PurposeEntry vector encoding non-tagged O-GlcNAc Transferase with open N-terminusDepositorInsertO-GlcNAc Transferase (OGT Human)
UseGateway cloningTagsExpressionMutationWT OGT with no start codon, in-frame with gateway…PromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
HEpl017
Plasmid#179992PurposepCBh_NLS_TadA8e_dSiCas12i_NLS_pA_pCMV_mCherry_pU6_crRNA_BbsI: vector for encoding a human codon-optimized dSiCas12i-ABE8e driven by CBh promoter and tagmCherry driven by CMV promoterDepositorInsertTadA8e-dSiCas12i(D1049A)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
CABE-T3.155
Plasmid#193270PurposeExpression of CABE-T3.155 base editor in mammalian cells, CMV promoterDepositorInsertCABE-T3.155
UseTagsExpressionMammalianMutationD10A (Cas9), TadA mutations withinPromoterCMVAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_KRAB-MeCP2_KanR neo
Plasmid#167901PurposePiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmidDepositorInsertdCas9-KRAB-MeCP2
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/MSCV-EGFP
Plasmid#82613PurposeExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseRetroviralTags3xFLAG tag and NLS (nuclear localization signal)ExpressionMutationhuman codon-optimized, D10A + H840APromoterLTRAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
AA031
Plasmid#216011PurposeFragmid fragment: (Cas protein) nickase Cas for base editingDepositorInsertnCas9-SpRY (D10A)_v1.1 [Sp]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
CBE-T1.14
Plasmid#193277PurposeExpression of CBE-T1.14 base editor in mammalian cells, CMV promoterDepositorInsertCBE-T1.14
UseTagsExpressionMammalianMutationD10A (Cas9), TadA mutations withinPromoterCMVAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/pMXs-neo
Plasmid#51260PurposeExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPR and RetroviralTags3xFLAG tag and NLS (nuclear localization signal)ExpressionMammalianMutationhuman codon-optimized, D10A + H840APromoterLTRAvailable sinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
PB-tight-DDdCas9VP192-T2A-GFP-IRES-Neo
Plasmid#102889PurposePiggyBac transposon system construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-T2A-GFP-IRES-Neo
UseCRISPRTagsExpressionMammalianMutationD10A, H840APromoterTRE-tightAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hA3F-BE3
Plasmid#113414PurposeExpresses hA3F-BE3 in mammalian cellsDepositorInserthA3F-BE3 (APOBEC3F Human, S. pyogenes and Bacteriophage PBS2)
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV_Npu-ABEmax N-terminal
Plasmid#137173PurposeExpresses the N-terminal split-intein fragment of ABEmax from the CMV promoterDepositorInsertNpu-ABEmax_N-terminal
UseTagsExpressionMammalianMutationCas9 D10APromoterCMVAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-nSpCas9
Plasmid#171691PurposeExpresses nSpCas9 in mammalian cellsDepositorInsertnSpCas9
UseCRISPRTagsExpressionMammalianMutationD10APromoterEF1aAvailable sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUF1-dCas9-GCN5
Plasmid#122509PurposeCombining with the specific sgRNA sequence to activate of the Plasmodium falciparum endogenous gene transcription, through the increasing of H3K9 acetylation.DepositorInsertdCas9-GCN5
UseCRISPRTags3*FLAGExpressionMutationdCas9(D10A,H840A)PromoterPfHsp86Available sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hA3D-BE3
Plasmid#113413PurposeExpresses hA3D-BE3 in mammalian cellsDepositorInserthA3D-BE3 (APOBEC3D Human, S. pyogenes and Bacteriophage PBS2)
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Target-AIDmax
Plasmid#139107PurposeMammalian Target-AIDmax expression vector (pSI-626)DepositorInsertBi_partite_NLS-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS
UseCRISPRTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
AA030
Plasmid#216010PurposeFragmid fragment: (Cas protein) nickase Cas for base editingDepositorInsertnCas9-SpG (D10A)_v1.1 [Sp]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
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TetO-FUW-VdC9BV
Plasmid#62195PurposeExpresses RNA-Guided, Nuclease-Inactive VP64:dCas9-BFP:VP64—VdC9BV—Fusion Protein to Enable Transactivation of Endogenous GenesDepositorInsertVP64dCas9BFPVP64
UseLentiviralTagsTwo VP64s tagged to dCas9 fused to BFPExpressionMammalianMutationD10A H840A (catalytically inactive) Cas9 (dCas9)PromoterAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMV_Npu-BE3.9max N-terminal
Plasmid#137171PurposeExpresses the N-terminal split-intein fragment of BE3.9max from the CMV promoterDepositorInsertNpu-BE3.9max_N-terminal
UseTagsExpressionMammalianMutationCas9 D10APromoterCMVAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV_Anc689-NG-nCas9
Plasmid#163527PurposeMammalian CG-to-GC base editingDepositorInsertAnc689-NG-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…PromoterAvailable sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV_eA3A-nCas9
Plasmid#163535PurposeMammalian CG-to-GC base editingDepositorInserteA3A-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)PromoterAvailable sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
CABE-T1.17
Plasmid#193260PurposeExpression of CABE-T1.17 base editor in mammalian cells, CMV promoterDepositorInsertCABE-T1.17
UseTagsExpressionMammalianMutationD10A (Cas9), TadA mutations withinPromoterCMVAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_LAMP1
Plasmid#111669PurposeMAC-tagged gene expressionDepositorInsertLAMP1 (LAMP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV_RBMX-eA3A-UdgX-nCas9
Plasmid#163546PurposeMammalian CG-to-GC base editingDepositorInsertRBMX-eA3A-UdgX-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)PromoterAvailable sinceDec. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV_hUbC-dSpCas9-2xVP64-T2A-BSD
Plasmid#162333PurposeLentiviral expression of dSpCas9-2xVP64 with blasticidin resistanceDepositorInsertdSpCas9-2xVP64
UseLentiviralTagsFLAGExpressionMammalianMutationD10A and H840APromoterhUbCAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET24a-ncOGT-TPR
Plasmid#190822PurposeFor expression of the TPR domain of human ncOGT (C467X mutation) in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.DepositorInsertO-GlcNAc Transferase (OGT Human)
UseTags6x HisExpressionBacterialMutationC467X. Also codon optimized for expression in E.c…PromoterT7Available sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
AALN-BE4max
Plasmid#138161PurposeC-to-T base editorDepositorInsertrAPOBEC1(AALN)-nSpCas9-UGI-UGI
UseTagsNLSExpressionMammalianMutationWithin rAPOBEC1 R33A + K34A + H122L + D124N; with…PromoterCMVAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/pMXs-IG
Plasmid#51258PurposeExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPR and RetroviralTags3xFLAG tag and NLS (nuclear localization signal)ExpressionMammalianMutationhuman codon-optimized, D10A + H840APromoterLTRAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-dCas9VPPH-T2A-GFP-shP53
Plasmid#102897PurposeEBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.DepositorInsertdCas9VPPH-T2A-GFP-shP53
UseCRISPRTagsExpressionMammalianMutationD10A, H840APromoterCAGAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJT25_GalL_BE3
Plasmid#143736PurposeExpresses BE3 in yeast cellsDepositorInsertBE3
UseCRISPRTagsExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV_eA3A-NG-nCas9
Plasmid#163536PurposeMammalian CG-to-GC base editingDepositorInserteA3A-NG-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…PromoterAvailable sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ266
Plasmid#164713PurposeGateway entry clone (attL1 & attR5) PmCDA1-CBE_V01 for C-T base editingDepositorInsertzCas9(D10A)-PmCDA1-UGI
UseCRISPR; Gateway compatible pmcda1-cbe_v01 entry c…TagsExpressionPlantMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
CABE-T2.19
Plasmid#193265PurposeExpression of CABE-T2.19 base editor in mammalian cells, CMV promoterDepositorInsertCABE-T2.19
UseTagsExpressionMammalianMutationD10A (Cas9), TadA mutations withinPromoterCMVAvailable sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
Sa-dCas9-NLS-3xFLAG/pcDNA3.1
Plasmid#98041PurposeExpresses Sa-dCas9-NLS-3xFLAG in mammalian cells for enChIP analysis to purify specific genomic regions of interestDepositorInsertSa-dCas9-NLS-3xFLAG
UseCRISPRTags3xFLAG tag and NLS (nuclear localization signal)ExpressionMammalianMutationD10A + H557APromoterCMVAvailable sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
HUWE1-sgRNA-1
Plasmid#86924PurposeTo express sgRNA target HUWE1 geneDepositorInsertHUWE1 sgRNA1 (HUWE1 Human)
UseTagsExpressionMammalianMutationPromoterU6Available sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV_Anc689-nCas9
Plasmid#163526PurposeMammalian CG-to-GC base editingDepositorInsertAnc689-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)PromoterAvailable sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
HUWE1-sgRNA-2
Plasmid#86925PurposeTo express sgRNA target HUWE1 geneDepositorInsertHUWE1 sgRNA2 (HUWE1 Human)
UseTagsExpressionMammalianMutationPromoterU6Available sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
Semaphorin7A-CD4d3+4-bio
Plasmid#73115PurposeEXPRESs plasmid for human erythrocyte surface proteins encoding Semaphorin7A with rat CD4d3+4-bioDepositorInsertSemaphorin 7A (SEMA7A Human)
UseTagsbiotinylation peptide and ratCD4d3+4ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUF1-dCas9-Sir2a
Plasmid#122510PurposeCombining with the specific sgRNA sequence to repress the Plasmodium falciparum endogenous gene transcription, through the decreasing of H3K9 acetylation.DepositorInsertdCas9-Sir2a
UseCRISPRTags3*FLAGExpressionMutationdCas9(D10A,H840A)PromoterPfHsp86Available sinceJune 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
CABE-T2.9
Plasmid#193264PurposeExpression of CABE-T2.9 base editor in mammalian cells, CMV promoterDepositorInsertCABE-T2.9
UseTagsExpressionMammalianMutationD10A (Cas9), TadA mutations withinPromoterCMVAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV_EE-nCas9
Plasmid#163541PurposeMammalian CG-to-GC base editingDepositorInsertEE-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)EE (R126E, R132E)PromoterAvailable sinceDec. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_21B
Plasmid#91129PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9_dead + AtU6:gRNA, Plant Selection: 2x35S:hpt IIDepositorInsertEngineering Reagent: 35S:AtCas9_dead + AtU6:gRNA
UseCRISPRTagsExpressionPlantMutationD10A, H840APromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK OGT (Neo)
Plasmid#154289PurposeLentiviral vector encoding full length wildtype human OGTDepositorInsertO-GlcNAc Transferase (OGT Human)
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_26G
Plasmid#91149PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:barDepositorInsertEngineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA
UseCRISPRTagsExpressionPlantMutationD10A, H840APromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only