Addgene

We narrowed to 6,831 results for: 11

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pMCSG7(gfcC)

Plasmid

#176197

Purpose

gfcC protein expression

Depositor

Mark Saper

Article

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcC

Use

Tags

6xHis-TeV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pEnt R3L2 TetO(fl)-2

Plasmid

#24416

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Type

Empty backbone

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

April 1, 2011

Availability

Academic Institutions and Nonprofits only

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pCXN2-mVIN

Plasmid

#11776

Depositor

Eileen Adamson

Article

Xu et al J Cell Sci. 1998 Jun . 111 ( Pt 11)():15

Insert

vinculin (Vcl Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.2 GW delCMV

Plasmid

#29496

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 29, 2011

Availability

Academic Institutions and Nonprofits only

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pHSP70B-CP6-TK

Plasmid

#165881

Purpose

Basic ganciclovir or acyclovir sensitvity cassette in Alpha2, lacks physical marker or attB site

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Basic Selection Vector

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pPromoterless-P2A

Plasmid

#165790

Purpose

Viral P2A part in promoter grammar, for building promoterless control vectors

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Promoterless-2A

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pN-PURO-PTP

Plasmid

#51711

Purpose

contains an N-terminal tandem affinity purification tag (relevant to any system) within a construct designed for genome integration into trypanosomes.

Depositor

Arthur Gunzl

Article

Schimanski et al Eukaryot Cell. 2005 Nov;4(11):1942-50.

Type

Empty backbone

Use

T. brucei genome integration vectors

Tags

PTP (ProtC-TEV-ProtA)

Expression

Mutation

Promoter

Available since

March 19, 2014

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmDCP1

Plasmid

#21684

Depositor

Elisa Izaurralde

Article

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

Decapping protein 1 (Dcp1 Fly)

Use

Drosophila expression

Tags

EGFP

Expression

Mutation

Promoter

Available since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pICSL80007

Plasmid

#50323

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, mCherry variant of RFP (Discosoma sp.)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pICSL50007

Plasmid

#50308

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

C terminal FLAG tag

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH75111

Plasmid

#50327

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, beta-glucuronidase (E. coli) with two introns,

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pLSG (stra8)

Plasmid

#21619

Depositor

David Garbers

Article

Dann et al Stem Cells. 2008 Nov . 26(11):2928-37.

Insert

Stra8 promoter (Stra8 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 25, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
M4758 KanMX::URA3 Disruptor Converter

Plasmid

#51688

Purpose

Yeast marker swap plasmid for converting KanMX3 to URA3

Depositor

David Stillman

Article

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

URA3 (URA3 Budding Yeast)

Use

Yeast marker swap

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pMCSG7(gfcB)

Plasmid

#176196

Purpose

gfcB protein expression

Depositor

Mark Saper

Article

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcB

Use

Tags

6xHis-TeV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA h-MLSN

Plasmid

#21083

Depositor

Craig Montell

Article

Xu et al Proc Natl Acad Sci U S A. 2001 Sep 11. 9

Insert

TrpM1 (TRPM1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pTMt_NES_TCS(Q’G)_HA-dCas9(N)_P2A-Puro-WPRE

Plasmid

#101101

Purpose

Encodes dCas9(N) fused to transmembrane tether

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

TMt-dCas9(N)

Use

CRISPR and Synthetic Biology

Tags

HA and Myc

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pICSL30005

Plasmid

#50299

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

N terminal FLAG tag (3x FLAG octapeptide)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pICSL50006

Plasmid

#50319

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, luciferase (Photinus pyralis)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pQLinkHD_EBFP2

Plasmid

#118853

Purpose

fluorescent protein expression with an N-terminal His-tag

Depositor

Marc Birtwistle

Article

Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

Insert

EBFP2

Use

Tags

His-tag

Expression

Bacterial

Mutation

EBFP1.2-I128V/V150I/ D155V/V224R

Promoter

Available since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH45152

Plasmid

#50263

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter, cab1 (AT1g29930, A. thaliana)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pEnt L1L3 EF1a-tTA-3

Plasmid

#24415

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Type

Empty backbone

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
M4757 KanMX::TRP1 Disruptor Converter

Plasmid

#51687

Purpose

Yeast marker swap plasmid for converting KanMX3 to TRP1

Depositor

David Stillman

Article

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

TRP1 (TRP1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pPKC1 (JCE575)

Plasmid

#89773

Purpose

yeast expression of PKC1

Depositor

Juan Carlos Igual

Article

Soriano-Carot et al Nucleic Acids Res. 2014 Jun;42(11):7084-

Insert

PKC1 (PKC1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

yeast PKC

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL-MLKif3B

Plasmid

#13744

Depositor

Linda Wordeman

Article

Ginkel et al Mol Biol Cell. 2000 Dec . 11(12):4143-58

Insert

Motorless Kif3B (Kif3b Mouse)

Use

Cre/Lox

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pAND_Reporter

Plasmid

#60591

Purpose

AND gate reporter

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

AND_Reporter

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 23, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pICSL30003

Plasmid

#50304

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, no stop codon, mCherry variant of RFP (Discosoma sp.)

Use

Tags

Expression

Plant

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pICSL30004

Plasmid

#50302

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS no stop codon, YFP variant of GFP (Aequorea victoria)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
M4753 KanMX::ADE2 Disruptor Converter

Plasmid

#51683

Purpose

Yeast marker swap plasmid for converting KanMX3 to ADE2

Depositor

David Stillman

Article

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

ADE2 (ADE2 Budding Yeast)

Use

Yeast marker swap

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
p3XFlag-CMV7-miniMAVS(KaganE48)

Plasmid

#52019

Purpose

Expresses miniMAVS (the sequence starting from M142 of the MAVS CDS) with an N-terminal 3X flag tag

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

miniMAVS

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
M3499 ura3::ADE2 Disruptor Converter

Plasmid

#51674

Purpose

Yeast marker swap plasmid for converting URA3 to ADE2

Depositor

David Stillman

Article

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

ADE2 (ADE2 Budding Yeast)

Use

Yeast marker swap

Tags

Expression

Yeast

Mutation

Promoter

ADE2

Available since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pMLS544

Plasmid

#188882

Purpose

Integrating Pmex-5::Cas9 expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

Pmex-5::Cas9

Use

Tags

Expression

Worm

Mutation

C. elegans codom optimization and intron addition

Promoter

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH71421

Plasmid

#51834

Purpose

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

plant 3'UTRpolyadenylation signal/terminator, Tom H4

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

March 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-myc-MmUbc7/2776

Plasmid

#11488

Depositor

Allan Weissman

Article

Tiwari et al J Biol Chem. 2001 May 11. 276(19):16193-

Insert

MmUbc7 (Ube2g2 Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

n/a

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
pICH42211

Plasmid

#50255

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter nos (A. tumefaciens)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pICSL50011

Plasmid

#50317

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, EOS photoactivatable fluorescent protein (L. hemprichii)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pICSL80001

Plasmid

#50326

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, luciferase (P. pyralis)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPYCeGM0001

Plasmid

#196141

Purpose

Gateway entry vector with Arabidopsis TGA1 CDS and c-terminal HiBiT tag

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

AtTGA1_HiBit

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pICSL80004

Plasmid

#50325

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, plant codon-optimsed variant of turbo RFP (Entacmaea quadricolor)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
M2371 his3::ADE2 Disruptor Converter

Plasmid

#51671

Purpose

Yeast marker swap plasmid for converting HIS3 to ADE2

Depositor

David Stillman

Article

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

ADE2 (ADE2 Budding Yeast)

Use

Yeast marker swap

Tags

Expression

Yeast

Mutation

Promoter

ADE2

Available since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pMLS546

Plasmid

#188883

Purpose

Integrating Pmex-5::Cas9 expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

Pmex-5::Cas9

Use

Tags

Expression

Worm

Mutation

C. elegans codom optimization

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19 PKC zeta

Plasmid

#8425

Depositor

Frederic Mushinski

Article

Goodnight et al Gene 1992 Dec 15;122(2):305-11.

Insert

Mouse Protein Kinase C zeta (Prkcz Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
TR-GFP

Plasmid

#80591

Purpose

Expresses GFP from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

Depositor

Aravind Asokan

Article

Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

Insert

GFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CBA

Available since

Sept. 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pICSL80016

Plasmid

#50332

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS no stop codon, beta-glucuronidase (E. coli) with two introns

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
Id2-Halo donor

Plasmid

#113116

Purpose

Donor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Id2 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Id2 donor region including homology arms and HaloTag

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH72400

Plasmid

#50338

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, Atug7 (A. tumefaciens)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBDKRB2_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE

Plasmid

#101108

Purpose

Encodes dCas9(N) fused to BDKRB2

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

BDKRB2-dCas9(N)

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAND_Int2

Plasmid

#60590

Purpose

A transcriptional AND circuit constructed by layering NOR and NOT gates

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

AND_Int2

Use

Tags

Expression

Bacterial

Mutation

Promoter

PPhlF

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
f1H (264)

Plasmid

#24425

Depositor

Steven Rosen

Article

Morimoto-Tomita et al Neoplasia. 2005 Nov . 7(11):1001-10.

Insert

SULF1 (SULF1 Human)

Use

Tags

FLAG, His, and Myc

Expression

Mammalian

Mutation

22aa N-terminal truncation

Promoter

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3 N-MLSN

Plasmid

#21082

Depositor

Craig Montell

Article

Xu et al Proc Natl Acad Sci U S A. 2001 Sep 11. 9

Insert

TRPM1 (TRPM1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA GAL4 CREB 1-341

Plasmid

#22972

Depositor

Marc Montminy

Article

Conkright et al Mol Cell. 2003 Apr . 11(4):1101-8.

Insert

CREB

Use

Tags

GAL4 DNA-binding domain

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only

Showing: 1251 - 1300 of 6831 results

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