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Plasmid#108552PurposeBacterial sgRNA expression vectorDepositorInsert2x Streptococcus pyogenes sgRNA
UseTagsExpressionBacterialMutationPromoterJ23119Available sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
ZO-1 shRNA 3253
Plasmid#37206DepositorInsertdog ZO-1 shRNA (TJP1 canine)
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
GEARBOCS-SPARCL1 KO
Plasmid#218182PurposeTo KO Hevin in astrocytesDepositorInsertsgRNA (Sparcl1 Mouse)
UseAAV and CRISPRTagsExpressionMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTK73.ChrV_oxTi365.gRNA
Plasmid#194052PurposesgRNA targeting ChrV_oxTi365 locus of the C. elegans genomeDepositorInsertChrV_oxTi365 sgRNA
UseTagsExpressionWormMutationPromoterU6Available sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR BFP-guide1
Plasmid#118157PurposeCRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the BFP CDSDepositorInsertKRAB-dCas9-P2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterEF1a core and U6Available sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT009.J1.J306
Plasmid#177325PurposescRNA targeting J3 promoter for activationDepositorInsertJ306
UseTagsExpressionMutationPromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
MS2-pegRNA
Plasmid#181811Purposems2 incorporated pegRNA-1.1DepositorInsertMS2-pegRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
GESTALT_pX330-v1
Plasmid#103061Purposeplasmid px330 with guide targeting GESTALT v1 through V5 constructsDepositorInsertintegration of Cas9 with guide targeting GESTALT barcodes V1 to V5
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBbS5c-AcrB/AcrD/AcrFa, ABO_0964
Plasmid#86206PurposeExpresses efflux pump in E. coli to provide resistance to biofuel (low copy)DepositorInsertEfflux pump
UseTagsExpressionBacterialMutationPromoterATCGTTTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAA…Available sinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Crispr-V2-HMID.v1
Plasmid#103062PurposeLentiCrisprV2 with guide targeting V1-V5 GESTALT barcodesDepositorInsertCas9 plus expressed GESTALT V1-V5 guide
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMM100
Plasmid#127225PurposeAtU6::sgRNA targeting PDSDepositorInsertsgRNA
UseTagsExpressionPlantMutationcodon optimized CDSsPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1371_sgNanog
Plasmid#63710PurposeExpress sgNanog in mammalian cells of mice.DepositorInsertsgNanog, CMV promoter, puro, mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
LRT2B-GO
Plasmid#136908PurposeLentivirus expressing GO sgRNADepositorInsertsgGO
UseLentiviralTagsExpressionMutationWTPromoterhU6Available sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX330.LoxP
Plasmid#66586PurposesgRNA for LSLDepositorInsertLoxP sgRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330.iGFP2
Plasmid#66582PurposesgRNA for iGFPDepositorInsertiGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBSU6-shTOM40.2
Plasmid#89882PurposeExpresses shRNA against TOM40DepositorInsertshRNA Tom40
UseTagsnoneExpressionMammalianMutationPromoterAvailable sinceJune 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330.LoxPO
Plasmid#66585PurposesgRNA for LSLDepositorInsertLSL-Tomato
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCD294.9
Plasmid#113319PurposedCas9, PCP-SoxS_R93A, J109 scRNA.b1DepositorInsertdCas9, PCP-SoxS_R93A, J109 scRNA.b1_PP7
UseCRISPRTagsExpressionBacterialMutationR93APromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-blast-cfRab25_4
Plasmid#26712DepositorInsertdog Rab25 RNAi
UseLentiviral and RNAiTagsExpressionMammalianMutationCanine Rab25 RNAi.PromoterAvailable sinceDec. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pQdCas12a.luxR(mut)-sggfp(A)
Plasmid#236039PurposeThe plasmid pQdCas12a.luxR(mut)-sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.DepositorInsertquorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
UseCRISPRTagsExpressionMutationPromoterquorum sensing promoterAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pABE-dGFP-gRNA
Plasmid#226864PurposeHuman gRNA expression vector targeting the A111V mutation of non-fluorescent EGFP in plasmid pABE-dGFP; for use with ABEsDepositorInsertSp-gRNA
UseTagsExpressionMammalianMutationPromoterhU6Available sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCBE-dGFP-gRNA
Plasmid#226862PurposeHuman gRNA expression vector targeting the Y93H mutation of non-fluorescent EGFP in plasmid pCBE-dGFP; for use with CBEsDepositorInsertSp-gRNA
UseTagsExpressionMammalianMutationPromoterhU6Available sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
p105_gRNA_Sa_CD40
Plasmid#213781PurposeExpresses CRISPRa gRNA for CD40 promoter and mScarlet. gRNA driven by mU6.DepositorInsertSa gRNA
UseCRISPR and LentiviralTagsExpressionMutationPromotermU6Available sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
lenti-ERCC1 gRNA2
Plasmid#211631PurposesgRNA-2 against ERCC1DepositorInsertsgRNA ERCC1
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDT4
Plasmid#199120Purposescrambled promoter circular DNA templateDepositorInsertsynthetic transcription template
UseTagsExpressionBacterialMutationmutated 5′-CTGGAGTGCG-3′ to 5′-CTGGAGACCG-3′ to …PromoterAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMpGE010-gR085
Plasmid#188554PurposeBinary vector for CRISPR/Cas9 targeted to MpIGPD in Marchantia polymorpha (for Agrobacterium-mediated genetic transformation)DepositorInsertgR085
UseCRISPR; Entry vectorTagsExpressionMutationPromoterAvailable sinceMay 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMpGE_En03-gR085
Plasmid#188552PurposeGateway entry vector for sgRNA targeted to MpIGPD.Transient expression of sgRNA targeted to MpIGPD in Marchantia polymorpha.DepositorInsertgR085
UseCRISPR; Entry vectorTagsExpressionMutationPromoterAvailable sinceMay 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgDcc
Plasmid#159906PurposeMutagenesis of DccDepositorInsertDcc (Dcc Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
PX458_THRA_iso1_2
Plasmid#128246PurposeEncodes gRNA for 3' target of human THRA_iso1DepositorInsertTHRA_iso1 gRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
PX458_ZBTB49_iso1_1
Plasmid#128250PurposeEncodes gRNA for 3' target of human ZBTB49_iso1DepositorInsertZBTB49_iso1 gRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only