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Plasmid#166741PurposeExpress Venus fused to the N-terminus of the Bcl-2 family protein, BimL with mutation in BH3 domain to disrupt binding anti-apoptotic proteinsDepositorInsertBimL-4E (Bcl2l11 Mouse)
UseTagsVenusExpressionMammalianMutation4 hydrophobic residues in the BH3 region mutated …PromoterCMVAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
4JDS
Plasmid#60081PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSETD7:DCC011-H01:C212434 (SETD7 Human)
UseTags6xHisExpressionBacterialMutationSee commentsPromoterT7Available sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
AAV:ITR-EF1aCore-SaCas9-dual(U6-sgRNA(backbone))-ITR
Plasmid#207878PurposeAAV vector encoding EF1a core promoter-driven SaCas9 and two sgRNA cloning sites w/ the engineered Sa Guide scaffold variant (BbsI and PaqCI for sgRNA cloning).DepositorTypeEmpty backboneUseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
plx304-PHB-V5
Plasmid#122230PurposeExpresses human PHB attached to a V5 tagDepositorInsertProhibitin (PHB1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV:ITR-EF1aCore-SaCas9-single(U6-sgRNA(backbone))-ITR
Plasmid#207880PurposeAAV vector encoding EF1a core promoter-driven SaCas9 and single sgRNA cloning site w/ the engineered Sa Guide scaffold variant (BbsI for sgRNA cloning).DepositorTypeEmpty backboneUseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO-PHB-8
Plasmid#122233PurposeExpresses shRNA targeting the coding sequence of human PHBDepositorInsertshRNA targeting PHB (see partial depositor seq) (PHB1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO-PHB-6
Plasmid#122232PurposeExpresses shRNA targeting the coding sequence of human PHBDepositorInsertshRNA targeting PHB (see partial depositor seq) (PHB1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast HA-GPAT4-ΔNs5
Plasmid#173169PurposeRetroviral vector to express sgRNA resistant HA tagged human GPAT4 with mutation in CHP1 binding siteDepositorInsertGlycerol-3-Phosphate Acyltransferase 4 (GPAT4 Human)
UseRetroviralTagsHAExpressionMutationSynonymous mutations at sgRNA sites, amino acids …PromoterMMLVAvailable sinceAug. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast 3XFLAG-CHP1-myr_mut
Plasmid#173167PurposeRetroviral vector to express sgRNA resistant 3XFLAG tagged human CHP1 with myristoylation site mutationDepositorInsertCalcineurin Like EF-Hand Protein 1 (CHP1 Human)
UseRetroviralTags3XFLAGExpressionMutationSynonymous mutations at sgRNA sites, G2A, S6APromoterMMLVAvailable sinceAug. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
Venus-BimEL(Bad)-pEGFP-C1
Plasmid#166758PurposeTo examine effect of swapping the BH3 region in full length BimEL with that of BadDepositorUseTagsVenusExpressionMammalianMutationBH3 region removed and inserted BH3 of human Bad …PromoterCMVAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
TJP3
Plasmid#25310PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertTJP3:G508-L808:cH6 (TJP3 Human)
UseTagsHisExpressionBacterialMutationContains amino acids G508-L808PromoterAvailable sinceApril 4, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLKO-PHB-4
Plasmid#122231PurposeExpresses shRNA targeting the 3' UTR of human PHBDepositorInsertPHB (PHB1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
KIF13B
Plasmid#25309PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertKIF13B:S4-K351:cH6 (KIF13B Human)
UseTagsHisExpressionBacterialMutationContains amino acids S4-K351PromoterAvailable sinceAug. 16, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
4MI0
Plasmid#60097PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertEZH2:JMC033-A03:C216172 (EZH2 Human)
UseBaculovirus expressionTags6xHisExpressionMutationSee commentsPromoterT7Available sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLXI_TRC403 LacZ
Plasmid#111184PurposeControl inducible vector with LacZ (V5 tagged)DepositorInsertlacZ
UseLentiviralTagsV5ExpressionMutationPromoterTREAvailable sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLXI_TRC401 LacZ
Plasmid#111183PurposeControl inducible vector with LacZ (V5 tagged)DepositorInsertlacZ
UseLentiviralTagsV5ExpressionMutationPromoterTREAvailable sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003 Luciferase
Plasmid#117072Purposesingle guide RNA targeting LuciferaseDepositorInsertLuciferase
UseCRISPRTagsExpressionMutationPromoterhU6Available sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET29b-clbF
Plasmid#114148PurposeExpresses ClbF-cHisDepositorInsertClbF
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b-clbE
Plasmid#114147PurposeExpresses ClbE-cHisDepositorInsertClbE
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b-clbD
Plasmid#114146PurposeExpresses ClbD-cHisDepositorInsertClbD
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003 PSMB5 guide 1
Plasmid#117073Purposesingle guide RNA targeting PSMB5; guide 1DepositorInsertLMPX (PSMB5 Human)
UseCRISPRTagsExpressionMutationPromoterhU6Available sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003 PSMB5 guide 2
Plasmid#117074Purposesingle guide RNA targeting PSMB5; guide 2DepositorInsertLMPX (PSMB5 Human)
UseCRISPRTagsExpressionMutationPromoterhU6Available sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003 UBE2C guide 1
Plasmid#117068Purposesingle guide RNA targeting UBE2C; guide 1DepositorInsertUBCH10 (UBE2C Human)
UseCRISPRTagsExpressionMutationPromoterhU6Available sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003 UBE2C guide 2
Plasmid#117071Purposesingle guide RNA targeting UBE2C; guide 2DepositorInsertUBCH10 (UBE2C Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGGF008
Plasmid#48848PurposeProvides a BASTA resistance cassette as GreenGate module.DepositorInsertpNOS::BastaR::tNOS
UseGolden gate compatible cloning vectorTagsExpressionMutationchi sequence in BastaR removed by silent substitu…PromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
T7-VEE-OKS-iG
Plasmid#58974PurposeRNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORFDepositorUseVenezuelan equine encephalitis (vee) virus rna re…TagsExpressionMammalianMutationPromoterAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
SP6-VEE-OKS-iM
Plasmid#58973PurposeRNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORFDepositorUseVenezuelan equine encephalitis (vee) virus rna re…TagsExpressionMammalianMutationPromoterAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
SP6-VEE-OKS-iG
Plasmid#58975PurposeRNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORFDepositorUseVenezuelan equine encephalitis (vee) virus rna re…TagsExpressionMammalianMutationPromoterAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLXI_TRC401 SMARCB1 var 2
Plasmid#111182PurposeInducible vector (untagged) with SMARCB1 variant 2DepositorInsertBAF47 (SMARCB1 Human)
UseLentiviralTagsExpressionMutationPromoterTREAvailable sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR SMARCB1 var 2
Plasmid#111181PurposeSMARCB1 variant 2 in pDONR223 backboneDepositorInsertBAF47 (SMARCB1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only