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Plasmid#13645DepositorInsertCD14 (CD14 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
plenti.CamKII.(cyto).iATPSnFR2.A95A.A119L.HaloTag
Plasmid#209724PurposeExpresses cytosolic low affinity ratiometric ATP sensorDepositorInsert(cyto).iATPSnFR2.A95A.A119L.HaloTag
UseLentiviralTagsExpressionMammalianMutationPromoterCamKIIAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTXTL-T7max-RLuc
Plasmid#188312PurposeExpression of Renilla luciferase via a T7 promoter in TXTLDepositorInsertRenilla luciferase
UseLuciferase and Synthetic BiologyTags8x His-tagExpressionBacterialMutationPromoterT7MaxAvailable sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-Tet1-CD
Plasmid#83340PurposeTo introduce dCas9-fused Tet1-CD and sgRNA2.0 systemDepositorInsertdCas9-Tet1-CD and sgRNA scaffold with 2xMS2 binding sites
UseCRISPRTagsExpressionMammalianMutationD10A, H840APromoterU6, CBHAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
TFORF1923
Plasmid#141889PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZBTB20 (ZBTB20 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rab35 WT
Plasmid#47424DepositorInsertRab35 (RAB35 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDH-TfR-mApple
Plasmid#179384PurposeLentiviral vector for expression of Human Transferrin receptor-1 fused mAppleDepositorInsertTransferrin receptor 1 - mApple (TFRC Synthetic, Human)
UseLentiviralTagsmAppleExpressionMammalianMutationPromoterEF1Available sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
SF-SON IRES Puro
Plasmid#214402PurposeExpresses human SOX10, OLIG2, NKX6.2 and PAC (puromycin N-acetyl-transferase)DepositorUseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBMN YFP-DHFR(DD)
Plasmid#29326DepositorInsertYFP-DHFR(DD)
UseRetroviralTagsHA and YFPExpressionMutationChange Arg 12 to His (R12H),Asn 18 Thr (N18T), Va…PromoterAvailable sinceApril 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSCAR_sgRNA_hygro-tagBFP-lox2272
Plasmid#162078Purpose3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and hygromycin resistanceDepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationPromoterU6, EF1aAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC1-HyPerRed-mito
Plasmid#60247PurposeMitochondria-targeted genetically encoded hydrogen peroxide indicator with red fluorescence.DepositorInsertHyPerRed-mito
UseTagsMitochondrial targeting signalExpressionMammalianMutationPromoterCMVAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
TAp63alpha-FLAG
Plasmid#27008DepositorInsertTAp63alpha (TP63 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDT386
Plasmid#174733PurposeExpresses His-tagged DT386, a truncated diphtheria toxin without a receptor-binding domain.DepositorInsertDT386
UseTagsHistagExpressionBacterialMutationDeleted amino acids 412-560 (the receptor binding…PromoterT5Available sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-mCherry
Plasmid#50462PurposeDouble floxed mCherry under the control of EF1a promoterDepositorInsertmCherry
UseAAVTagsN/AExpressionMutationPromoterEF1aAvailable sinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA
Plasmid#154868PurposeHuman synapsin-1 promoter; Flp-dependent hM3D(Gq) DREADD-mCherryDepositorHas ServiceAAV Retrograde and AAV8InserthM3D(Gq)-mCherry
UseAAV; Flp-frtTagsmCherryExpressionMammalianMutationPromoterHuman Synapsin-1Available sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual wt p110α/p85α
Plasmid#199302Purposeco-expression of His-tagged p110α wt and Avi-tagged p85α wt proteinsDepositorUseTagsAvi and HisExpressionInsectMutationPromoterAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMP71-HLA-A0201-His
Plasmid#108214PurposeHLA-A0201 his taggedDepositorInsertmajor histocompatibility complex, class I, B (HLA-A Human)
UseRetroviralTagshisExpressionMammalianMutationPromoterAvailable sinceDec. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-mSNX27
Plasmid#163617Purposemouse SNX27 cDNA N-terminally tagged with GFPDepositorInsertmouse SNX27 (Snx27 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpCas9-P2A-EGFP (RTW3027)
Plasmid#139987PurposeCMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationPromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmtb-t7-alpha-bungarotoxin
Plasmid#69542PurposeFor in vitro transcription of alpha-bungarotoxin mRNA that can be injected into embryos.DepositorInsertalpha-bungarotoxin
UseTol2, beta-actin promoter, sp6,t7 for zebrafishTagsExpressionMutationPromoterbeta-actin, sp6, t7Available sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
Twinkle-APEX2-V5
Plasmid#129705PurposeAPEX2 fused to the mitochondrial nucleoid protein TwinkleDepositorUseLentiviralTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICE-RNaseHI-WT-NLS-mCherry
Plasmid#60365PurposePlasmid for expression of bacterial RNase HI tagged with NLS-mCherry that can be used to degrade R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.DepositorInsertRNase HI
UseTagsNLS from SV40 T antigen and mCherryExpressionMammalianMutationPromoterCMV-tetAvailable sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE17
Plasmid#90359PurposeNFAT - PKC/Ca++ gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterNFATAvailable sinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_rDA-mut
Plasmid#140558PurposeExpresses the red fluorescent DA-insensitive control sensor GRAB_rDA-mut in neuronsDepositorHas ServiceAAV9InsertRed fluorescent DA-insensitive sensor GRAB_rDA-mut (control sensor)
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
AttB_mCherry
Plasmid#171598PurposeStrong mCherry fluorescence from Bxb1 landing padDepositorInsertmCherry
UseSynthetic Biology; Promoterless bxb1 dna recombin…TagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTXTL-T7max-FLuc
Plasmid#188311PurposeExpression of Firefly luciferase via a T7 promoter in TXTLDepositorInsertFirefly luciferase
UseLuciferase and Synthetic BiologyTags8x His-tagExpressionBacterialMutationPromoterT7MaxAvailable sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCold-I-Dsup
Plasmid#90021PurposeTo express His-tagged Dsup in bacteriaDepositorInsertDsup
UseTagsHisExpressionBacterialMutationPromotercspAAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.FAPdL5-POST-T2A-dTomato.WPRE.bGH
Plasmid#105981PurposeThis AAV plasmid constitutively expresses an extracellular dL5 FAP fused to the neuroligin-1 cytoplasmic domain for targeting to the synapse with a cell fill dTomato.DepositorInserthSyn-Igkappa-myc-FAPdL5-POST-T2A-dTomato-WPRE-bGH
UseAAVTagsFAPdL5-POST, T2A-dTomato, and mycExpressionMutationPromoterhuman synapsin 1Available sinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF2258
Plasmid#142927PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSOX11 (SOX11 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1362 scFv entry plasmid
Plasmid#201912PurposeEntry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)DepositorInsertStuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only