Addgene

We narrowed to 6,716 results for: 11

Showing: 5141 - 5160 of 6716 results

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RV-Cag-Dio-GFP

Plasmid

#87662

Purpose

retroviral vector encoding cre dependent expression of GFP

Depositor

Oscar Marin

Article

Ciceri et al Nat Neurosci. 2013 Sep;16(9):1199-210. d

Insert

eGFP

Use

Retroviral

Tags

Expression

Mutation

Promoter

CAG

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pLVX-PERK(LD)-EGFP-HOTag3

Plasmid

#225678

Purpose

Used to generate stable cell lines that express PERK(LD)-EGFP-HOTag3 and to detect the unfolded proteins in the ER lumen.

Depositor

Likun Wang

Article

Xu et al Cell Rep. 2024 Jun 25;43(6):114358. doi:

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pGTR

Plasmid

#63143

Purpose

PCR template to construct polycistronic tRNA-gRNA

Depositor

Yinong Yang

Article

Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Insert

gRNA-tRNA fusion

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-UtrCH

Plasmid

#26737

Depositor

William Bement

Article

Burkel et al Cell Motil Cytoskeleton. 2007 Nov . 64(1

Insert

Utrophin

Use

Reporter for f-actin

Tags

GFP

Expression

Mammalian

Mutation

Calponin homology domain of utrophin (aa# 1-261).…

Promoter

CMV IE94

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pGS-21a his6-GST-WTPhi29DNAP

Plasmid

#166863

Purpose

To produce a dual affinity-tagged Phi29 DNA polymerase

Depositor

Stephen Quake, Paul Blainey

Article

Blainey et al Nucleic Acids Res. 2011 Mar;39(4):e19. d

Insert

Phi29 Polymerase codon-optimized for overexpression in E. coli

Use

Tags

GST and his6

Expression

Bacterial

Mutation

Promoter

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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SypHer mt

Plasmid

#48251

Purpose

pH sensor, targeted to mitochondria

Depositor

Nicolas Demaurex

Article

Poburko et al J Biol Chem. 2011 Apr 1;286(13):11672-84

Insert

SypHer

Use

Tags

2 mitochondrial targeting sequences

Expression

Mammalian

Mutation

C199S

Promoter

CMV

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pCAH01Sp::Cas9

Plasmid

#128160

Purpose

Ptet-controlled expression of S. pyogenes Cas9

Depositor

Calvin Henard

Article

Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

Insert

Sp Cas9

Use

Tags

Expression

Bacterial

Mutation

Promoter

Ptet

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-postASAP

Plasmid

#178792

Purpose

Expresses voltage sensor targeted to dendrites and spines

Depositor

Rafael Yuste

Article

Cornejo et al Science. 2022 Jan 7;375(6576):82-86. doi

Insert

FingR.PSD95 and ASAP2f

Use

Tags

Expression

Mammalian

Mutation

mutations in amino acids L146G, S147T N149R, S150…

Promoter

CAG

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pInt

Plasmid

#18941

Depositor

Michele Calos

Article

Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

Insert

phiC31 integrase (int Streptomyces phage phiC31)

Use

Tags

Expression

Bacterial

Mutation

under lacZ promoter

Promoter

Available since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only

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AICSDP-117:POLR2A-mEGFP

Plasmid

#164500

Purpose

Homology arms and mEGFP-linker sequence sequence for N-terminus tagging of human POLR2A

Depositor

Allen Institute for Cell Science

Insert

POLR2A Homology Arms with mEGFP-linker (POLR2A Human)

Use

CRISPR; Donor template

Tags

mEGFP-linker

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Promoter

Available since

Feb. 17, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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PtxS1-c003

Plasmid

#173076

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

PtxS1

Use

Tags

Expression

Bacterial

Mutation

D35-I221

Promoter

Available since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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JAB2121

Plasmid

#190343

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (empty vector control)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pNIC-CFP

Plasmid

#173074

Purpose

Expression vector for E. coli producing target protein with N-terminal CFP fusion

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Type

Empty backbone

Use

Tags

mCerulean

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pTA-attB

Plasmid

#18937

Depositor

Michele Calos

Article

Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

Insert

phiC31 attB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2008

Availability

Academic Institutions and Nonprofits only

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AICSDP-144:CTCF-mEGFP

Plasmid

#168799

Purpose

Homology arms and mEGFP-linker sequence sequence for N-terminus tagging of human CTCF

Depositor

Allen Institute for Cell Science

Insert

CTCF (CTCF Human)

Use

CRISPR; Donor template

Tags

mEGFP-linker

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Promoter

Available since

June 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pMA-MsgRNA-EGFP

Plasmid

#80794

Purpose

For insertion of gRNA array containing 11-30 gRNA modules

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pUC119 T7 hTRmin

Plasmid

#127858

Purpose

T7 RNA Pol for in vitro transcription

Depositor

Kathleen Collins

Article

Wu et al EMBO J. 2014 Apr 16;33(8):921-35. doi: 1

Insert

hTR (TERC Human)

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pTA-attP

Plasmid

#18939

Depositor

Michele Calos

Article

Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

Insert

phiC31 attP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-Nck1

Plasmid

#45892

Depositor

Louise Larose

Article

Kebache et al Proc Natl Acad Sci U S A. 2002 Apr 16;99

Insert

Nck-1 (NCK1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
pCAH01Sp::Cas9D10A

Plasmid

#128161

Purpose

Ptet-controlled expression of S. pyogenes Cas9D10A variant

Depositor

Calvin Henard

Article

Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

Insert

Sp Cas9

Use

Tags

Expression

Bacterial

Mutation

D10A

Promoter

Ptet

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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