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Plasmid#52636Purposemammalian expression of HA tagged HIF1alpha (full length) with P402A,P564A mutationDepositorInsertHIF1alpha P402A, P564A (HIF1A Human)
UseTagsHAExpressionMammalianMutationP402A, P564APromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pC0053-CMV-dPspCas13b-GS-ADAR2DD(E488Q)-delta-984-1090
Plasmid#103869PurposedPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAVDepositorInsertsUseCRISPRTagsGS and HIV NESExpressionMammalianMutationChanged histidne 133 to alanine and histidine 105…PromoterAvailable sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
Pet28a_Human_H4
Plasmid#42633DepositorInsertHuman H4 (H4C1 Human)
UseTagsUntaggedExpressionBacterialMutationPromoterT7Available sinceFeb. 25, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDF0234 pCMV - huDisCas7-11 dead mutant
Plasmid#172509PurposeEncodes human codon optimized catalytically dead DisCas7-11 protein alone for expression in mammalian cellsDepositorInsertpCMV - huDisCas7-11 dead mutant
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQFlag-USP7 CS puroR
Plasmid#46752PurposeRetroviral vector that expresses catalytically inactive form of Flag-tagged human USP7DepositorInsertUSP7 (USP7 Human)
UseRetroviralTagsFlagExpressionMammalianMutationC223S--catalytically inactivePromoterCMVAvailable sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-EGFP-NLS
Plasmid#86677PurposeExpresses nuclear-localized GFP after lentiviral transduction. Can be used to monitor GFP leakage into the cytosol following nuclear envelope rupture events.DepositorInsertEGFP-NLS
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
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Tandem p40p35
Plasmid#108665PurposeExpression of bioactive mouse IL-12 as a tandem dimer of p40 and p35 linked with a Gly-Ser linkerDepositorUseTagsExpressionMammalianMutationIL-12 dimers have a Gly-Ser linkerPromoterAvailable sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-SARS-CoV-2-nsp8-2xStrep-IRES-Puro
Plasmid#141374PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-nsp8 (ORF1ab SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 9, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFP-Sec23A
Plasmid#66609PurposeGFP fusion of Sec23ADepositorInsertSec23A (SEC23A Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-HA-hUBE1L
Plasmid#12438DepositorInsertUBE1L (UBA7 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHAGE PGK-GFP-IRES-LUC-W
Plasmid#46793Purpose2nd generation lentiviral vectorDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HDAC4-FLAG
Plasmid#30485DepositorInsertHDAC4 (HDAC4 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pC1-SypHer3s-IMS
Plasmid#108120PurposeMitochondrial ratiometric pH probe with enhanced brightness fused to Smac/DIABLO fragment. Probe is localized in mitochondrial intermembrane space. Mammalian cell expression.DepositorInsertSypHer3s-IMS
UseTagsratiometricYFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV2.5-THP-GFP
Plasmid#80336PurposeFluorescent Reporter for Dopaminergic Neuron DifferentiationDepositorInsertrat TH promoter (Th Rat)
UseAAVTagsExpressionMutationPromoterrat Tyrosine hydroxylaseAvailable sinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJFRC28-10XUAS-IVS-GFP-p10
Plasmid#36431DepositorInsertGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-CMV-iRFP
Plasmid#31856Purposea phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imagingDepositorInsertiRFP
UseAdenoviralTagsExpressionMammalianMutationT2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…PromoterCMVAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV-polysite
Plasmid#122843PurposeGateway destination vector for mammalian expression without tag, or for adding custom tags by conventional cloning. Distinct multiple cloning sites 5' and 3' of gateway cassette.DepositorTypeEmpty backboneUseGateway destinationTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-Akt DN (K179M)
Plasmid#16243DepositorInsertAkt1 (Akt1 Mouse)
UseTagsHAExpressionMammalianMutationDominant negative (K179M)PromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTALYM4SpMi-05
Plasmid#47880PurposeTALE-mRuby2 against Mus spretus/musculus minor satellite sequenceDepositorInsertTALE-mRuby2
UseTALENTagsmRuby2ExpressionMammalianMutationPromoterCAGAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only