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pAAV-5U6-sgRNAs-hsyn-EGFP

Plasmid

#112213

Purpose

Targeted DNA methylation

Depositor

Xingxu Huang

Article

Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

Insert

sgRNAs

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pEU3-NII-GLICNot-C-BIOT

Plasmid

#140186

Purpose

Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, GST-tag and biotinylation site, and NotI restriction site

Depositor

Tamás Mészáros

Article

Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

Type

Empty backbone

Use

Expression vector for a cell-free system

Tags

Biotinylation and GST

Expression

Mutation

Promoter

T7

Available since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pEG302 22aa SunTag VP64 gRNA17 (FWA)

Plasmid

#120250

Purpose

CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA

Depositor

Steven Jacobsen

Article

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

g17_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-mCherry-FHA1-subT391A-mCherry

Plasmid

#104462

Purpose

Cre-dependent, Non-phosphorylatable PKA consensus substrate tagged with mCherry

Depositor

Bernardo Sabatini

Article

Chen et al Neuron. 2017 Dec 6;96(5):1070-1083.e5. d

Insert

mCherry-subT391A-FHA1-mCherry

Use

AAV

Tags

mCherry

Expression

Mutation

Promoter

CAG

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCas59

Plasmid

#82396

Purpose

sgRNA targeting YFP +787 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Use

Tags

Expression

Mutation

Promoter

PEZ3

Available since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pCas57

Plasmid

#82395

Purpose

sgRNA targeting YFP +111 from TSS; template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Use

Tags

Expression

Mutation

Promoter

PEZ3

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas56

Plasmid

#82394

Purpose

sgRNA targeting YFP +46 from TSS; template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Use

Tags

Expression

Mutation

Promoter

PEZ3

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas55

Plasmid

#82393

Purpose

sgRNA targeting YFP +172 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Use

Tags

Expression

Mutation

Promoter

PEZ3

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas52

Plasmid

#82392

Purpose

sgRNA targeting YFP +52 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Use

Tags

Expression

Mutation

Promoter

PEZ3

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pAN-OR-MalT-3NT

Plasmid

#62309

Purpose

synthetic circuit: OR gate, the sgRNA used for the NOT gate was substituted with one designed to target the malT gene in the E. coli genome

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Inserts

A2NT sgRNA
Malt-3NT sgRNA
A2NT sgRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PA2, PPhlF, and pBAD

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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Gal4pBS3-Pleum

Plasmid

#60330

Purpose

Contains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 11, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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Gal4pBS1-Pleum

Plasmid

#59993

Purpose

Contains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 11, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pHL 1410

Plasmid

#53011

Purpose

pLtetO-1:DsrA, pCon:ompC::T7noRBS(900bps), pLlacO-1:rpoS::gfp

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Inserts

DsrA sRNA
ompC
rpoS

Use

Synthetic Biology

Tags

T7noRBS (900bp) and gfp

Expression

Bacterial

Mutation

contains −150 to +30 bp relative to start codon a…

Promoter

pCon, pLlacO-1, and pLtetO-1

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pHL1853

Plasmid

#53040

Purpose

PLlacO-1:glgC::gfp::LVA, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st2):csrA

Depositor

Han Lim

Article

Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

Inserts

GFP
tetR
csrA

Use

Synthetic Biology

Tags

LVA, RBS(st2), RBS(st3), and glgC leader

Expression

Bacterial

Mutation

Promoter

pConNoHind and pLlac0-1

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pHL 1075

Plasmid

#53004

Purpose

pCon:fhlA::mCherry, pLlacO-1:sodB::gfp

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Inserts

fhlA
sodB

Use

Synthetic Biology

Tags

GFP and mCherry

Expression

Bacterial

Mutation

contains −309 to +96 bp relative to start codon a…

Promoter

pCon and pLlacO-1

Available since

May 16, 2014

Availability

Academic Institutions and Nonprofits only
pHL 1035

Plasmid

#52999

Purpose

pLtetO-1:RhyB, pCon:fhlA::mCherry, pLlacO-1:sodB::gfp

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Inserts

RhyB sRNA
fhlA
sodB

Use

Synthetic Biology

Tags

gfp and mCherry

Expression

Bacterial

Mutation

contains −309 to +96 bp relative to start codon a…

Promoter

pCon, pLlacO-1, and pLtetO-1

Available since

May 16, 2014

Availability

Academic Institutions and Nonprofits only
pHL 1033

Plasmid

#52998

Purpose

pLtetO-1:RhyB, pCon:rpoS::mCherry, pLlacO-1:sodB::gfp

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Inserts

RhyB sRNA
rpoS
sodB

Use

Synthetic Biology

Tags

gfp and mCherry

Expression

Bacterial

Mutation

contains −150 and +30 bp relative to start codon …

Promoter

pCon, pLlacO-1, and pLtetO-1

Available since

May 16, 2014

Availability

Academic Institutions and Nonprofits only
pHL 1073

Plasmid

#53003

Purpose

pCon:ompC::mCherry, pLlacO-1:sodB::gfp

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Inserts

mCherry
GFP

Use

Synthetic Biology

Tags

ompC (target mRNA) and sodB (target mRNA)

Expression

Bacterial

Mutation

contains −56 to +141 bp relative to start codon a…

Promoter

pCon and pLlacO-1

Available since

May 16, 2014

Availability

Academic Institutions and Nonprofits only
pHL 1303

Plasmid

#53008

Purpose

pLtetO-1:sodB::mCherry, pLlacO-1:ompC::gfp

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Inserts

sodB
ompC

Use

Synthetic Biology

Tags

GFP and mCherry

Expression

Bacterial

Mutation

contains -56 to +141 relative to start codon and …

Promoter

pLlacO-1 and pLtetO-1

Available since

May 14, 2014

Availability

Academic Institutions and Nonprofits only
pVRa04_1617

Plasmid

#49658

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF04_1617

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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