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SAE2-mGarnet
Plasmid#175240PurposeBacterial expression and purification, combined with SAE1 to form the E1 enzyme heterodimerDepositorInsertUBA2 (UBA2 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
FKBP-EGFP-RanGAP
Plasmid#175236PurposeBacterial expression and purification, high affinity SUMOylation substrate that can recruited to FRB with rapamycinDepositorInsertRANGAP1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-RanGAP
Plasmid#175235PurposeBacterial expression and purification, high affinity SUMOylation substrateDepositorInsertRANGAP1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMbd3EosHaloTAF_Hyg
Plasmid#140958PurposeTargeting construct to make Mbd3-Eos-Halo-Tev-Avi-3xFlag fusionDepositorInsertmethyl-CpG binding protein 3 (Mbd3 Mouse, Synthetic)
UseMouse TargetingTags3xFLAG, Avi, Eos, Halo, and TEVExpressionMutationPromoterAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pChd4EosHaloTAF_Puro
Plasmid#172099PurposeTargeting construct to make Chd4-Eos-Halo-Tev-Avi-3xFlag fusionDepositorInsertchromodomain helicase DNA binding protein 4 (Chd4 Mouse, Synthetic)
UseMouse TargetingTags3xFLAG, Avi, Eos, Halo, and TEVExpressionMutationPromoterAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH578
Plasmid#172162PurposepylBCD variant in pBAD backboneDepositorInsertPara (pylBCD 3f2_v2); AraC
UseTagsExpressionBacterialMutationPylB(N61S, E84K, E122K, E178K, G188R, E251D) PylC…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH581
Plasmid#172165PurposepylBCD variant in pBAD backboneDepositorInsertPara (pylBCD 3f2+C); AraC
UseTagsExpressionBacterialMutationPylB(N61S, E84K, E122K, E178K, G188R, E251D) PylC…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH767
Plasmid#172172PurposePylB overexpression and purificationDepositorInsertPT7/Lac-[His6-TEV-SUMO-PylB.WT]
UseTagsExpressionBacterialMutationpET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylB]PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH857
Plasmid#172175PurposePylB overexpression and purificationDepositorInsertPT7/Lac-[His6-TEV-SUMO-PylB.WTopt]
UseTagsExpressionBacterialMutationpET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBopt]PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH202
Plasmid#172150PurposeAccessory Plasmid for PACE of pylBCD variantsDepositorInsertPpsp gIII.1TAG, luxAB; Plpp 32A; PproK pylT
UseTagsExpressionBacterialMutationgIII(P29*); 32A pylS variantPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH223
Plasmid#172151PurposepylBCD variant in pCDF backboneDepositorInsertPT7/lac (pylBCD 34B_sub-pop5); LacI
UseTagsExpressionBacterialMutationPylB(N61S, E178K, E251D) PylD(A252V)PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH224
Plasmid#172152PurposepylBCD variant in pCDF backboneDepositorInsertPT7/lac (pylBCD 40C_sub-pop6); LacI
UseTagsExpressionBacterialMutationPylB(N61S, E175K, E178K)PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH463
Plasmid#172155PurposepylBCD variant in pBAD backboneDepositorInsertPara (pylBCD 36A_sub-pop1); AraC
UseTagsExpressionBacterialMutationPylB(N61S, E122K, E178K, G188R)PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH573
Plasmid#172157PurposepylBCD variant in pBAD backboneDepositorInsertPara (Untagged pylBCD); AraC
UseTagsExpressionBacterialMutationCodon-optimized Methanosarcina acetivorans pylBCDPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH574
Plasmid#172158PurposepylBCD variant in pBAD backboneDepositorInsertPara (pylBCD 36A_sub-pop2); AraC
UseTagsExpressionBacterialMutationPylB(N61S, E84K, E122K) PylC(E316D)PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH575
Plasmid#172159PurposepylBCD variant in pBAD backboneDepositorInsertPara (pylBCD 34B_sub-pop3); AraC
UseTagsExpressionBacterialMutationSumo(S6L) PylB(N61S, E178K, G188R, E251D) PylD(A2…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH576
Plasmid#172160PurposepylBCD variant in pBAD backboneDepositorInsertPara (pylBCD 34B_sub-pop4); AraC
UseTagsExpressionBacterialMutationPylB(N61S, E175K, E178K, E251D) PylD(A252V)PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH19
Plasmid#172141PurposeAccessory Plasmid for PACE of pylBCD variantsDepositorInsertPpsp gIII.1TAG, luxAB; Plpp pylS; PproK pylT
UseTagsExpressionBacterialMutationgIII(P29*)PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH60
Plasmid#172142PurposeMicroscopy inclusion body assayDepositorInsertpCDF.CD
UseTagsExpressionBacterialMutationPylCD partial operon (PylB deleted to assess impa…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only
