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pSIL-eGFP-shACTN4

Plasmid

#52679

Purpose

shRNA against α-actinin-4 with eGFP transfection marker

Depositor

Johannes Hell

Article

Schnizler et al J Biol Chem. 2009 Jan 30;284(5):2697-705

Insert

ACTN4 (Actn4 Rat)

Use

RNAi

Tags

CMV-EGFP

Expression

Mutation

Promoter

U6

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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pAG303GPD-ccdB-EGFP

Plasmid

#14182

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Available since

March 15, 2007

Availability

Academic Institutions and Nonprofits only

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pLC242-GFP-Sestrin2

Plasmid

#100519

Purpose

Expresses GFP tagged Sestrin2 in mammalian cells to make lentivirus

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

SESN2 (SESN2 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 28, 2017

Availability

Academic Institutions and Nonprofits only

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TNF-SBP-EGFP_puromycin

Plasmid

#65284

Purpose

TNF reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

TNF (TNF Human)

Use

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

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DHFR-GFP-th-GluA1

Plasmid

#173008

Purpose

For light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and GluA1.

Depositor

Matthew Kennedy

Article

Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

DHFR-GFP-th-GluA1

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

human synapsin

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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BiDi-[mCherry2]mEGFP

Plasmid

#182874

Purpose

Bi-directional expression vector of monomers

Depositor

Salvatore Chiantia

Article

Petazzi et al J Virol. 2020 Nov 25. pii: JVI.01238-20.

Insert

mEGFP, mCherry2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2 Chfr (1014)

Plasmid

#61853

Purpose

mammalian expresion of Chfr fused to EGFP

Depositor

Jonathon Pines

Article

Matsusaka et al J Cell Biol. 2004 Aug 16;166(4):507-16.

Insert

Chfr (CHFR Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 17, 2015

Availability

Academic Institutions and Nonprofits only
mPA-GFP-Fibrillarin-7

Plasmid

#57134

Purpose

Localization: Nucleoli, Excitation: 400 / 504, Emission: 515 / 517

Depositor

Michael Davidson

Insert

Fibrillarin (FBL Human)

Use

Tags

mPA-GFP

Expression

Mammalian

Mutation

R36Q, S66F

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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8798 GFP-KrasG12C_2B_hygro

Plasmid

#64376

Purpose

This a retroviral expression plasmid expressing GFP tagged G12C mutant Kras 2B oncogene along with hygro resistance gene

Depositor

Georgios Stathopoulos

Article

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Insert

mouse Kras2B (Kras Mouse)

Use

Retroviral

Tags

GFP

Expression

Mutation

changed glycine 12 to cysteine

Promoter

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-Rab21-CCSS

Plasmid

#83424

Purpose

Mouse Rab21, mutation of the putative COOH-terminal prenylation motif (residues 218 and 219 from C to S). Generated from pEGFP-Rab21 by site-directed mutagenesis.

Depositor

Johanna Ivaska

Article

Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.

Insert

Rab21 (Rab21 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Residues 218 and 219 from C to S (CCSS: TGCTGT -&…

Promoter

CMV

Available since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pBI121-GFP-RmMYB108

Plasmid

#173181

Purpose

Expresses Rosa multiflora MYB108 for subcellular localization in plants

Depositor

Daidi Che

Article

Dong et al Front Plant Sci. 2021 Jul 27;12:696919.

Insert

RmMYB108

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-siRNA control

Plasmid

#10900

Depositor

Bob Weinberg

Article

Yang et al Cell 2004 Jun 25;117(7):927-39.

Insert

siGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 17, 2006

Availability

Academic Institutions and Nonprofits only
pBS-f1(-)-TOMM20-mEGFP

Plasmid

#115923

Purpose

donor to insert EGFP at the TOMM20 locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

TOMM20-mEGFP

Use

Donor template

Tags

EGFP

Expression

Mutation

Promoter

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pAG425GPD-EGFP-ccdB

Plasmid

#14322

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

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LV-tetO-EF1a-GFP

Plasmid

#187645

Purpose

Lentiviral vector expressing GFP from EF1a promoter downstream from tetO sites

Depositor

Martin Carroll

Insert

GFP

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLV.5’-LMNA-eGFP-KI

Plasmid

#170545

Purpose

Expresses a sgRNA for 5' tagging to LMNA and contains a cassette with eGFP flanked by homology arms for LMNA

Depositor

Jacob Giehm Mikkelsen

Article

Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

Inserts

Left Homology arm for LMNA knockin
Right Homology arm for VIM knockin

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLV.5’VIM-eGFP-KI

Plasmid

#170547

Purpose

Expresses a sgRNA for 5' tagging to VIM and contains a cassette with eGFP flanked by homology arms for VIM

Depositor

Jacob Giehm Mikkelsen

Article

Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

Inserts

Left Homology arm for VIM knockin
Right Homology arm for VIM knockin

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_mGFP-FGFR3-mScarlet

Plasmid

#192509

Purpose

Expression of N-terminally mGFP and C-terminally mScarlet labelled wildtype FGFR3 (isoform IIIc)

Depositor

Irene Tiemann-Boege

Article

Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

Insert

mGFP-FGFR3IIIc-mScarlet

Use

Tags

mGFP and mScarlet

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pBS-f1(+)-ACTB-mEGFP

Plasmid

#115909

Purpose

donor to insert EGFP at the ACTB locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

ACTB-mEGFP (ACTB Human)

Use

Donor template

Tags

EGFP

Expression

Mutation

Promoter

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-GFP-MKLP1

Plasmid

#140566

Purpose

Expresses GFP tagged MLKP1

Depositor

Heidi Hehnly

Article

Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

Insert

MKLP1 (KIF23 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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