-
Plasmid#188567Purposegreen biosensor for cAMP in mammalian cellsDepositorInsertG-Flamp1
UseTagsHis tagExpressionMammalianMutationPromoterCAGAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2 neo
Plasmid#98292PurposeVariant of lentiCRISPRv2 that confers G418 resistanceDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterEF-1aAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2 puro
Plasmid#98290PurposeVariant of lentiCRISPRv2 that confers puromycin resistanceDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterEF-1aAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-G-Flamp1-mut
Plasmid#188568Purposecontrol probe of G-Flamp1, insensitive to cAMPDepositorInsertG-Flamp1-mut
UseTagsHis tagExpressionMammalianMutationPromoterCAGAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Tet-On-shRNA-Control
Plasmid#98398PurposeLentivirus for expression of non-targeting control shRNA (Dox-inducible)DepositorInsertnon-targeting randomized sequence
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-OptoSTIM1
Plasmid#70159PurposeMammalian expression plasmid of OptoSTIM1 (optogenetically-activated STIM1 protein)DepositorInsertOptoSTIM1 (STIM1 Human, Mustard Weed)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV2-hSyn-G-Flamp1
Plasmid#188569Purposegreen biosensor for cAMP in neuronsDepositorInsertG-Flamp1
UseAAVTagsHis tagExpressionMutationPromoterhSynAvailable sinceOct. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV2-hSyn-G-Flamp1-mut
Plasmid#188570Purposecontrol probe of G-Flamp1, insensitive to cAMPDepositorInsertG-Flamp1-mut
UseAAVTagsHis tagExpressionMutationPromoterhSynAvailable sinceOct. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEJS1099: Dual-sgRNA.Design 4
Plasmid#159537PurposeDelivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vectorDepositorInsertNme2Cas9 with two guide RNA cassettes
UseAAV, CRISPR, and Mouse TargetingTagsNLSExpressionMammalianMutationPromoterU1aAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRRL_SRSF2_WT_mCherry
Plasmid#84020Purposeexpression of SRSF2 in mammalian cellsDepositorInsertSRSF2 (SRSF2 Human)
UseLentiviralTags2TA-mCherry and FlagExpressionMammalianMutationPromoterPGKAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
ABE8e-Cas9NG
Plasmid#203328PurposePlasmid for bacterial purification of codon optimized ABE8e-Cas9NGDepositorInsertABE8e-Cas9NG
UseCRISPRTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
lenti-SpCas9 puro
Plasmid#104994PurposeThis 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.DepositorInsertKpnI-XhoI-BsrGI-NheI
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
lenti-SpCas9 neo
Plasmid#104996PurposeThis lentiviral construct delivers hSpCas9 and G418 resistance.DepositorInsertKpnI-XhoI-BsrGI-NheI
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
eTd-CBE
Plasmid#196601PurposeMammalian expression of eTd-CBEDepositorInsertbpNLS-TadA-8e (N46L)-nSpCas9-bpNLS-P2A-UGI-bpNLS
UseTags6*HisExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
LV-AR
Plasmid#213819PurposeLenti-viral expression of human androgen receptor wild-type (WT).DepositorInsertAR (human androgen receptor) (AR Human)
UseLentiviralTagsFlag (N terminal on backbone)ExpressionMammalianMutationNo, it is AR wild-type.PromoterEF-1aAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
lenti-SpCas9 hygro
Plasmid#104995PurposeThis lentiviral construct delivers hSpCas9 and hygromycin resistance.DepositorInsertKpnI-XhoI-BsrGI-NheI
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBABE-MCS(extended)-Puro (empty vector)
Plasmid#172603PurposeEmpty retroviral vector for the constitutive, near-physiological expression of a gene of interest and a puromycin resistance marker in mammalian cells.DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationThe multiple cloning site (MCS) has been edited/e…PromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
pCold-I-Dsup
Plasmid#90021PurposeTo express His-tagged Dsup in bacteriaDepositorInsertDsup
UseTagsHisExpressionBacterialMutationPromotercspAAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only