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Showing: 5121 - 5140 of 9067 results

  1. pEC1.2/EC1.1_spCas9_GFP

    Plasmid
    #161933
    Purpose
    CRISPR plasmid with spCas9 expressed under egg cell promoter - GFP seed selection
    Depositor
    Christian Fankhauser
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    161933-plasmid-map-sequence-id-320699

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  2. pEC1.2/EC1.1_spCas9_TagRFP

    Plasmid
    #161934
    Purpose
    CRISPR plasmid with spCas9 expressed under egg cell promoter - TagRFP seed selection
    Depositor
    Christian Fankhauser
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    161934-plasmid-map-sequence-id-320739

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  3. pNGFP-BC

    Plasmid
    #161956
    Purpose
    Used for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).
    Depositor
    Eric Gouaux
    Article
    Kawate et al Structure. 2006 Apr;14(4):673-81. doi: 1
    Type
    Empty backbone
    Use
    Tags
    GFPuv His8
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    161956-plasmid-map-sequence-id-319761

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  4. pCGFP-BC

    Plasmid
    #161959
    Purpose
    Used for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).
    Depositor
    Eric Gouaux
    Article
    Kawate et al Structure. 2006 Apr;14(4):673-81. doi: 1
    Type
    Empty backbone
    Use
    Tags
    GFPuv His8
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    161959-plasmid-map-sequence-id-319762

    Enlarge

  5. pNGFP-EU

    Plasmid
    #161967
    Purpose
    Used for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).
    Depositor
    Eric Gouaux
    Article
    Kawate et al Structure. 2006 Apr;14(4):673-81. doi: 1
    Type
    Empty backbone
    Use
    Tags
    EGFP His8
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    161967-plasmid-map-sequence-id-319758

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  6. pCGFP-EU

    Plasmid
    #161968
    Purpose
    Used for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).
    Depositor
    Eric Gouaux
    Article
    Kawate et al Structure. 2006 Apr;14(4):673-81. doi: 1
    Type
    Empty backbone
    Use
    Tags
    EGFP His8
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    161968-plasmid-map-sequence-id-319729

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  8. pMSR0

    Plasmid
    #161984
    Purpose
    Allele-Coupled Exchange for chromosomal manipulation of Clostridioides difficile ribotype 027 and other ribotypes. Uses inducible expression of CD2517.1 toxin as a counter-selection marker
    Depositor
    Olga Soutourina
    Article
    Peltier et al Commun Biol. 2020 Nov 27;3(1):718. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    161984-plasmid-map-sequence-id-321926

    Enlarge

  14. pFastBac1-His10FLAG

    Plasmid
    #157736
    Purpose
    empty pFastBac1 vector (polyhedrin promoter) adds enterokinase-removable N-terminal His10-tag + 3xFLAG tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites
    Depositor
    Debra Hansen
    Article
    Olson et al Protein Expr Purif. 2021 May 7:105890. d
    Type
    Empty backbone
    Use
    Tags
    His10 + 3xFLAG, enterokinase-cleavable
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Availability
    Academic Institutions and Nonprofits only
    157736-plasmid-map-sequence-id-333926

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  15. pMBPcyto-TEV-BseRI

    Plasmid
    #157739
    Purpose
    empty vector adds TEV-protease-removable N-term His10 + maltose binding protein (without signal sequence); avoids unwanted residues in the final protein sequence by using the BseRI restriction sites
    Depositor
    Debra Hansen
    Type
    Empty backbone
    Use
    Tags
    His10 + MBP (cytoplasmic expression; no signal se…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    157739-plasmid-map-sequence-id-334003

    Enlarge

  16. pT7-pelB

    Plasmid
    #157740
    Purpose
    empty vector; N-terminal PelB signal sequence; potential C-terminal His6; T7 promoter; like pET-20b, but additionally avoids added residues in final protein since cloning uses BseRI restriction sites
    Depositor
    Debra Hansen
    Type
    Empty backbone
    Use
    Tags
    PelB signal sequence and possible His6-tag (adds …
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    157740-plasmid-map-sequence-id-334002

    Enlarge

  17. pelBHisTEV

    Plasmid
    #157741
    Purpose
    empty vector; TEV-protease-removable N-term PelB signal sequence +His10; potential C-term His6; T7lac promoter; Kan-R; avoids added residues in final protein since cloning uses BseRI restriction sites
    Depositor
    Debra Hansen
    Type
    Empty backbone
    Use
    Tags
    PelB signal sequence + His10-tag + TEV protease c…
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac
    Availability
    Academic Institutions and Nonprofits only
    157741-plasmid-map-sequence-id-334005

    Enlarge

Showing: 5121 - 5140 of 9067 results