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Showing: 10241 - 10280 of 39906 results

  9. Anti-KCNT1/Slo2.2/Slack K+ channel [N3/26R]

    Plasmid
    #140064
    Purpose
    Mammalian Expression Plasmid of anti-KCNT1/Slo2.2/Slack K+ channel (Rat). Derived from hybridoma N3/26.
    Depositor
    James Trimmer
    Article
    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1
    Insert
    anti-KCNT1/Slo2.2/Slack K+ channel (Rat) recombinant mouse monoclonal antibody (Kcnt1 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    dual CMV
    Available since
    June 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    140064-plasmid-map-sequence-id-332077

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  23. Anti-KCNT2/Slo2.1/Slick K+ channel [N11/33R]

    Plasmid
    #114496
    Purpose
    Mammalian Expression Plasmid of anti-KCNT2/Slo2.1/Slick K+ channel (Mouse). Derived from hybridoma N11/33.
    Depositor
    James Trimmer
    Article
    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1
    Insert
    anti-KCNT2/Slo2.1/Slick K+ channel (Mus musculus) recombinant mouse monoclonal antibody (Kcnt2 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    dual CMV
    Available since
    Feb. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    114496-plasmid-map-sequence-id-236144

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  28. Cer(1-158)-beta-1 in pcDNAI/Amp

    Plasmid
    #55707
    Purpose
    An amino-terminal fragment of mCerulean was fused to Gbeta1. When co-expressed with a carboxyl terminal CFP fragment fused to a Ggamma subunit, a fluorescent signal s produced.
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    mCer(1-158)-beta 1 (GNB1 Aequorea victoria, Human)
    Use
    Tags
    Cer(1-158) was fused to the amino terminus of Gbe…
    Expression
    Mammalian
    Mutation
    GBeta-1 was amplified via PCR, which removed an i…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    55707-plasmid-map-sequence-id-114830

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  29. Cer(159-238)-beta-5 in pcDNAI/Amp

    Plasmid
    #55778
    Purpose
    A carboxyl-terminal mCerulean fragment was fused to Gbeta-5. When co-expressed with an amino terminal mCerulean fragment fused to a Ggamma subunit, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    Cer(159-238)-beta-5 (GNB5 Aequorea victoria, Human)
    Use
    Tags
    mCer(159-238) was fused to the amino terminus of …
    Expression
    Mammalian
    Mutation
    GBeta-5 was amplified via PCR, which added an N-t…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    55778-plasmid-map-sequence-id-114869

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  38. pAB2076 pAAV REPAIR.t1

    Plasmid
    #176323
    Purpose
    pAAV EFS-HIVNES-dCas13bt1- (GGS)2-huADAR2(E488Q)-3xHA BGHpolyA::U6-BpiI-Cas13bt1 DR (full REPAIR.t1 + crRNA expression for AAV production)
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103
    Inserts
    human codon optimized Cas13bt1 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    Cas13bt1 crRNA + BpiI cloning site
    Use
    AAV and CRISPR
    Tags
    3xHA and HIV NES
    Expression
    Mammalian
    Mutation
    E488Q, only the deaminase domain (aa 276-702 are …
    Promoter
    EFS (short EF1alpha) and hU6
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    176323-plasmid-map-sequence-id-345286

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  40. pdCas9-DNMT3A-PuroR (ANV)

    Plasmid
    #71684
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Synthetic, Human, S. pyogenes)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71684-plasmid-map-sequence-id-129672

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Showing: 10241 - 10280 of 39906 results