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Plasmid#165157PurposeExpresses NLS-AncBE4max-NLS with an N-terminal His Tag (6xHis) for bacterial expressionDepositorInsertAncBE4max
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF0863
Plasmid#143732PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertGTF2I (GTF2I Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
CAG-rtTA
Plasmid#185562PurposeUbiquitous expression of reverse tetracycline transactivator for TetON systemDepositorInsertrtTA
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Mito-7
Plasmid#55102PurposeLocalization: Mitochondria, Excitation: 587, Emission: 610DepositorInsertMito (COX8A Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
p7SL-neo-TET
Plasmid#51285Purposeexpresses human 7SL RNA, using its own polIII enhancer, and tagged with the self splicing intron neo cassetteDepositorInsert7SL RNA (RN7SL2 Human)
UseTagsneoTET cassette with a tetrahymena self-splicing …ExpressionMammalianMutationPromoter7SL upstream pollII enhancerAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLDPuro-hsnSR100N
Plasmid#35172DepositorInsertSRRM4 (SRRM4 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
hCas9
Plasmid#41815PurposeExpresses human codon optimized Cas9 nuclease for genome engineeringDepositorInsertCas9
UseCRISPRTagsExpressionMammalianMutationhuman codon-optimizedPromoterCMVAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EGFR
Plasmid#116731PurposeLentiviral expression of EGFRDepositorInsertEGFR (EGFR Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-IG-mSA
Plasmid#80706PurposeExpresses monomeric streptavidin (mSA) in bacterial cellsDepositorInsertMonomeric streptavidin
UseTags10xHis tag and TEV siteExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
FLAG-FOXO3a TM
Plasmid#8361DepositorInsertFOXO3a (FOXO3 Human)
UseTagsFlagExpressionMammalianMutationT32A, S253A, S315APromoterAvailable sinceFeb. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
TRPML1-G-GECO1.2-ERES
Plasmid#207144PurposeFusion of Ca2+ reporter and lysosomal Ca2+-permeable channel, with ER-export signalDepositorInsertMcoln1 (MCOLN1 Human)
UseTagsER-Export Signal (ERES) and G-GECO1.2ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
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Flag FoxO3
Plasmid#14937DepositorInsertFoxO3 (FOXO3 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGWB-cLUC
Plasmid#174051PurposeGateway compatible plasmid containing C-terminal luciferase for split-luciferase complementation assayDepositorTypeEmpty backboneUseLuciferaseTagsExpressionPlantMutationPromoterAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
ER-GCaMP6f
Plasmid#182548PurposeExpresses GCaMP6f on the cytosolic side of the ER (P-450site)DepositorInsertER localized P450
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
mOrange2-pBAD
Plasmid#54531PurposeLocalization: Protein Expression Vector , Excitation: 549, Emission: 565DepositorTypeEmpty backboneUseTags6xHis and mOrange2ExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pB2-neo-TET
Plasmid#51287Purposeexpresses the rodent SINE, B2, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassetteDepositorInsertsine B2
UseTagsneoTET cassette with a tetrahymena self-splicing …ExpressionMammalianMutationPromoterenhancer from 7SL geneAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
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AAV2/9 REP-AAP-ΔCAP
Plasmid#205991PurposeRep and AAP protein expression for AAV CREATE librariesDepositorInsertAAV9 Rep and AAP proteins for CREATE libraries
UseAAVTagsExpressionMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-puro/RAF1
Plasmid#131725PurposeMammalian Expression of RAF1DepositorInsertRAF1 (RAF1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only