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Plasmid#182569PurposeHuman fibronectin fragment, type-III repeats 8-10, R1379A mutantDepositorInsertHuman fibronectin fragment, type-III repeats 8-10
UseTagsHis and TEV cleavage site after His tagExpressionBacterialMutationR1379APromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR_full-site
Plasmid#186277PurposeReporter plasmid for full-site selection assayDepositorInsertThiomicrospira leader repeat and modified chloramphenicol selection cassette (removed RBS and start codon))
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe3-RfA-HA
Plasmid#186374PurposeDestination vector for the expression of a fusion of an N-ter protein of interest with an HA tag into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe3-HA-RfA
Plasmid#186373PurposeDestination vector for the expression of a fusion of N-ter HA tag and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe3-RfA-Venus
Plasmid#186372PurposeDestination vector for the expression of a fusion of an N-ter protein of interest with Venus into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe3-Venus-RfA
Plasmid#186371PurposeDestination vector for the expression of a fluorescent fusion of N-ter Venus and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe3-RfA
Plasmid#186370PurposeDestination vector for the expression of a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLBC0120
Plasmid#172791PurposeLevel 0 module containing CDS2ns_mHSP21DepositorInsertCDS mature HSP21 (44-227), Arabidopsis thaliana
UseSynthetic BiologyTagsExpressionMutationnoPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB520
Plasmid#185102Purpose1st 200 amino acids (N-term + transmembrane domain) of Pom152 fused to HA and GFPDepositorInsertPOM152
UseTagsGFPExpressionYeastMutationPOM152(1-200)-GFPPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB521
Plasmid#185103Purpose1st 960 amino acids (C-terminal truncation) of Pom152 fused with HA and GFPDepositorInsertPOM152
UseTagsGFPExpressionYeastMutationPOM152(1-960)-HA-GFPPromoterAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB382
Plasmid#185085PurposeHA-tagged POM152 with glycosylation sites mutatedDepositorInsertPOM152
UseTagsHAExpressionYeastMutationPom152 N280A, N398A, N???A, N???A = Pom152DglycPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR
Plasmid#186276PurposePlasmid containing shortened version of upstream Thiomicrospira CRISPR array for integration assaysDepositorInsertThiomicrospira shortened CRISPR array
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-KON206-MXMT
Plasmid#182729PurposeTransient expression of KON206-MXMT in plant cellDepositorInsertKON206
UseTagsExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-KOC169-MXMT
Plasmid#182728PurposeTransient expression of KOC169-MXMT in plant cellDepositorInsertKOC169
UseTagsExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-KON154-MXMT
Plasmid#182725PurposeTransient expression of KON154-MXMT in plant cellDepositorInsertKON154
UseTagsExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-KOC155-MXMT
Plasmid#182726PurposeTransient expression of KOC155-MXMT in plant cellDepositorInsertKOC155
UseTagsExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-KON168-MXMT
Plasmid#182727PurposeTransient expression of KON168-MXMT in plant cellDepositorInsertKON168
UseTagsExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLBT0180
Plasmid#172814PurposeLevel 1 module containing TP-CFP_P4 FDepositorInsertp35S-5UTMV-CTPSSU-CFP-3U+TER35S
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLBC0140
Plasmid#172794PurposeLevel 0 module containing CDS2ns_mCHERRYDepositorInsertCDS mCHERRY
UseSynthetic BiologyTagsExpressionMutationnoPromoterAvailable sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLBC0081
Plasmid#172787PurposeLevel 0 module containing CT_nVenusDepositorInsert3xFLAG CtmVenus 210-238 STOP pAGM1301 (CT)
UseSynthetic BiologyTagsExpressionMutationnoPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only