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pXD024 - pLKO-EF1a-GFP-P2A-FLuc-WPRE

Plasmid

#192204

Purpose

Lenti-GL(GFP-Luciferase)

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Lenti-GL(GFP-Luciferase)

Use

Lentiviral; Mammalian expression

Tags

Expression

Mutation

NA

Promoter

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-psMEK1

Plasmid

#89361

Purpose

A lenti-viral vector expressing photoswitchable MEK1 in mammalian cells

Depositor

Michael Lin

Article

Zhou et al Science. 2017 Feb 24;355(6327):836-842.

Insert

psMEK1 (MAP2K1 Synthetic, Human)

Use

Lentiviral

Tags

Human influenza hemagglutinin (HA) tag and pdDron…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti spCas9 T2A mNeonGreen P2A puro

Plasmid

#122183

Purpose

The plasmid codes for a Flag-spCas9 protein, a mNeonGreen fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.

Depositor

Raphael Gaudin

Inserts

spCas9 (NEWENTRY )
mNeonGreen

Use

Lentiviral

Tags

T2A

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-Kif5c-Myc

Plasmid

#186616

Purpose

Third generation lentiviral vector expressing Kinesin Heavy Chain 5 isoform C fused to Myc tag

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Kinesin Heavy Chain isoform 5c (KIF5C Human)

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLV Tmem161a-V5

Plasmid

#175149

Purpose

Lentiviral expression of mouse Tmem161a-V5

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Tmem161a (Tmem161a Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO-shp38

Plasmid

#52920

Purpose

expresses a hairpin specific for human p38MAPK

Depositor

Sheila Stewart

Article

Alspach et al Cancer Discov. 2014 Mar 26.

Insert

shRNA targeting mitogen-activated protein kinase p38 (MAPK14 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 9, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti-smURFP-T2A-mCherry

Plasmid

#80347

Purpose

Lentiviral transfer vector under the CMV promoter that expresses smURFP and mCherry.

Depositor

Erik Rodriguez, Roger Tsien

Article

Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

Inserts

smURFP
mCherry

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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PCBP1_pLX307

Plasmid

#98362

Purpose

Lentiviral expression of PCBP1

Depositor

Sefi Rosenbluh, William Hahn

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

PCBP1 (PCBP1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

E1Fa

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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PGK-IKZF3-NTERM-GFP

Plasmid

#185774

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, IKZF3 minidegron

Expression

Mutation

none

Promoter

PGK

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pSLIK CA ROCK2

Plasmid

#84649

Purpose

Lentiviral expression of doxycycline-inducible constitutively active ROCK2 and co-expression of Venus

Depositor

Sanjay Kumar

Article

Wong et al Cancer Res. 2015 Mar 15;75(6):1113-22. d

Insert

ROCK2 (ROCK2 Bovine)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Constitutive Active (please see depositor comment…

Promoter

Available since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti6-DEST PINK1-V5 G309D

Plasmid

#13318

Depositor

Mark Cookson

Article

Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

Insert

PINK1 (PINK1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

G309D

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
P-Ef1a-mCherry-T2A-RPL22-1xV5

Plasmid

#170324

Purpose

Expresses V5 and mCherry tagged RPL22

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_KRAS(G12V)-P2A-Hygro_Barcode

Plasmid

#170237

Purpose

Barcoded lentiviral vector to express KRAS (G12V) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

KRAS (KRAS Human)

Use

Lentiviral

Tags

Expression

Mutation

G12V

Promoter

EF1a

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-scFv-GCN4-HaloTag-GB1-NLS-dWPRE

Plasmid

#106303

Purpose

The plasmid encodes the scFv against GCN4 peptide fused with HaloTag and a NLS for SunTag imaging

Depositor

James Zhe Liu

Article

Liu et al Proc Natl Acad Sci U S A. 2017 Dec 28. p

Insert

scFv-GCN4-HaloTag-GB1-NLS

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLX304_zeo_mmFbxw7_R505C

Plasmid

#160104

Purpose

Expresses murine Fbxw7 alpha with the R505C loss-of-function mutation in mammalian cells

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Fbxw7 alpha R505C (Fbxw7 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

R505C

Promoter

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1aL-Cre-IRES-NeoR-W

Plasmid

#126700

Purpose

lentiviral or plasmid based co-expression of Cre recombinase and a neomycin resistance cassette

Depositor

Darrell Kotton

Article

Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

Insert

Cre-IRES

Use

Cre/Lox and Lentiviral

Tags

SV40 NLS

Expression

Mutation

Promoter

Ef1a

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCW57.1-mDux-CA

Plasmid

#99284

Purpose

All-in-one lentivector with tet-inducible mouse Dux (codon sequences altered)

Depositor

Stephen Tapscott

Article

Whiddon et al Nat Genet. 2017 Jun;49(6):935-940. doi:

Insert

Dux-CA (mouse; codon altered) (Dux Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pLX304-YAP1 _TA

Plasmid

#59143

Purpose

YAP1 mutants that harbor a deletion of the transcriptional activation domain

Depositor

William Hahn

Article

Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

TA (transactivation) domain deleted; aa291-497

Promoter

CMV

Available since

Oct. 23, 2014

Availability

Academic Institutions and Nonprofits only
pCDH-CB

Plasmid

#72267

Purpose

Express gene of interest under CB promoter

Depositor

Kazuhiro Oka

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

chicken beta-actin + CMV enhancer

Available since

Jan. 11, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pLV_Lyn11-mTurquoise2-iLID

Plasmid

#161000

Purpose

Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to lipidated peptide from LYN

Depositor

Sean R Collins

Article

Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

Insert

Lyn11-mTurquoise2-iLID

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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