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pGC200

Plasmid

#19623

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Ppro-1<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<1xNLS-lacZ::unc-54(3’)

Use

Flp/frt

Tags

Expression

Worm

Mutation

Promoter

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pGC160

Plasmid

#19632

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R1-R2)

Use

Flp/frt, destination vector

Tags

Expression

Worm

Mutation

Promoter

Available since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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pGC162

Plasmid

#19633

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R1-R2)<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)

Use

Flp/frt, destination vector

Tags

Expression

Worm

Mutation

Promoter

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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His-PHD2-181-426

Plasmid

#223551

Purpose

His-tagged human PHD2 catalytic domain with TEV cleavage site between His and PHD2 for PHD2 purification

Depositor

Somshuvra Mukhopadhyay

Article

Gurol et al Proc Natl Acad Sci U S A. 2024 Jun 25;12

Insert

PHD2 (EGLN1 Human)

Use

Tags

6x His

Expression

Bacterial

Mutation

Deleted amino acids 1-180.

Promoter

trc

Available since

Sept. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1(nsp8-nsp7)(nsp12)

Plasmid

#165451

Purpose

Expresses the RNA Dependent RNA polymerase complex of SARS-CoV-2 in Escherichia Coli

Depositor

Marc Delarue

Article

Madru et al PLoS One. 2021 Apr 29;16(4):e0250610. do

Inserts

nsp12
nsp8
nsp7

Use

Tags

14Histidine-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH1-CMV-HT-LC3-SV40-Hygro

Plasmid

#182045

Purpose

Lentiviral expression vector encoding HaloTag-LC3 for monitoring autophagosome closure

Depositor

Hong-Gang Wang

Article

Takahashi et al J Cell Biol. 2019 Oct 7;218(10):3336-335

Insert

MAP1LC3B (MAP1LC3B Human)

Use

Lentiviral

Tags

HaloTag-TEV

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pGD-P1/HC-Pro

Plasmid

#196328

Purpose

35S promoter-driven expression of the tobacco etch virus P1/HC-Pro RNA silencing suppressor

Depositor

Zhenghe Li

Article

Ganesan et al J Virol. 2013 Oct;87(19):10598-611. doi:

Insert

tobacco etch virus P1/HC-Pro

Use

Tags

Expression

Plant

Mutation

Promoter

duplicated cauliflower mosaic virus 35S promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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ASC-CASP1 Octamer

Plasmid

#164032

Purpose

Bacterial expression vector encoding a 5GSS-linked ASC(CARD)-CASP1(CARD) construct to express an octamer

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CASP1, ASC (CASP1, PYCARD Human)

Use

Tags

His6-MBP-TEV

Expression

Bacterial

Mutation

W169G (ASC), G20K (CASP1)

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCAD29_hIRG1_4-461_pvp008

Plasmid

#124843

Purpose

Expresses human CAD (Irg1) in E.coli

Depositor

Konrad Buessow

Article

Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

Insert

cis-aconitate decarboxylase (ACOD1 Human)

Use

Tags

StrepTag and TEV site

Expression

Bacterial

Mutation

deleted amino acids 1-3 and 462-end.

Promoter

T7

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pJJGL004

Plasmid

#180608

Purpose

Plasmid expressing E. coli codon optimized His-MBP-TEV-PlmCasX+Region1 insertion

Depositor

Jennifer Doudna

Article

Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

Insert

PlmCasX with DpbCasX R1 loop

Use

CRISPR

Tags

10xHis and MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pMAL-SH3(2)-5R

Plasmid

#112089

Purpose

Bacterial expression plasmid containing His and MBP tags for 5 repeats of the second SH3 domain from human NCK1.

Depositor

Michael Rosen

Article

Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

Insert

SH3(2)-5R (NCK1 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

construct contains only repeats of the second SH3…

Promoter

Lac

Available since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCAD39_mIRG1_4-462_pvp008

Plasmid

#124873

Purpose

Expresses mouse CAD (Irg1) in E.coli

Depositor

Konrad Buessow

Article

Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

Insert

cis-aconitate decarboxylase (Acod1 Mouse)

Use

Tags

StrepTag and TEV site

Expression

Bacterial

Mutation

deleted amino acids 1-3 and 463-end.

Promoter

T7

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac-GFP-dynein-2(D1091–Q4307)

Plasmid

#64064

Purpose

Codon-optimized human dynein-2 motor domain (D1091–Q4307) for baculovirus expression. 6His-ZZ-GFP N-terminal tag, TEV site to cleave 6His-ZZ.

Depositor

Andrew Carter

Article

Schmidt et al Nature. 2015 Feb 19;518(7539):435-8. doi

Insert

Cytoplasmic dynein-2 (DYNC2H1 Human)

Use

Tags

GFP, His tag, and ZZ tag

Expression

Insect

Mutation

Deleted amino acids 1-1090, extra C-terminal vali…

Promoter

Polyhedrin

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a Cdk2ap1CAN

Plasmid

#178032

Purpose

Expression vector for the purpose of protein purification.

Depositor

Lin He

Article

Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

Insert

Cdk2ap1 (Cdk2ap1 Mouse)

Use

Nonviral

Tags

6xHIS - Thrombin - MBP- TEV-TRS

Expression

Bacterial

Mutation

Promoter

T7LacI

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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LIC1B_Caspase-LOVN7C-7 C450A

Plasmid

#104628

Purpose

bacterial expression of light-activated caspase-3 with N7C-7 linker variation on C450A mutation

Depositor

Jim Wells

Article

Smart et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

Insert

Caspase-3 (CASP3 Human)

Use

Tags

His and LOV2

Expression

Bacterial

Mutation

C450A (dark-locked and light-insensitive mutation)

Promoter

T7

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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His-MBP human SPOP (aa 28-337)

Plasmid

#52294

Purpose

bacterial expression of human SPOP (aa 28-337) fused to His-MBP

Depositor

Brenda Schulman

Article

Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

Insert

SPOP (SPOP Human)

Use

Tags

His-MBP and TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 11, 2014

Availability

Academic Institutions and Nonprofits only
pCAG-AviMta2-3xFiBsd

Plasmid

#140964

Purpose

full-length wt Avi-Mta2-3XFLAG-iBsd cDNA w/CAG promoter and Blasticidin resistance, cloned from mouse ES cells

Depositor

Brian Hendrich

Article

Burgold et al EMBO J. 2019 Jun 17;38(12). pii: embj.20

Insert

metastasis-associated gene family, member 2 (Mta2 Mouse)

Use

Tags

3xFLAG, Avi, and TEV

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCD5-H5_Vietnam_1203/04_Y161A_sfGFP_ST

Plasmid

#182547

Purpose

Expresses a sfGFP fused H5 protein of the prototype A/Vietnam/1203/04 virus with a Y161A mutations conferring Neu5Gc specificity

Depositor

Robert de Vries

Article

Broszeit et al Cell Rep. 2019 Jun 11;27(11):3284-3294.e

Insert

H5_Vietnam_1203_04_Y161A

Use

Tags

GCN4-TEV-sfGFP-TwinStrep

Expression

Mammalian

Mutation

Y161A mutant that confers exclusive NeuAc to NeuG…

Promoter

CMV

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCAD54_CAD29_H104A_pvp008

Plasmid

#124876

Purpose

Expresses hCAD His103Ala mutant in E.coli

Depositor

Konrad Buessow

Article

Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

Insert

cis-aconitate decarboxylase (ACOD1 Human)

Use

Tags

StrepTag and TEV site

Expression

Bacterial

Mutation

mutated His103 to Ala, deleted amino acids 1-3 an…

Promoter

T7

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pET28-MHL: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2

Plasmid

#167849

Purpose

Bacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNA

Depositor

Cheryl Arrowsmith

Article

Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

Insert

nsp10, nsp16

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 22, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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