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Plasmid#148544PurposeBacterial Expression of SpEDC1opt_155-180DepositorInsertSpEDC1opt_155-180
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-Rluc-6xbsMS2-random_AC
Plasmid#148431PurposeMammalian Expression of 6xbsMS2-randomDepositorInsert6xbsMS2-random
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-HsNanos2-del6-22_X
Plasmid#147961PurposeMammalian Expression of HsNanos2-del6-22DepositorInsertHsNanos2-del6-22 (NANOS2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
RP51A-No5'ss-NoBS-NoPseudoBS
Plasmid#100987PurposeRP51A with T7 promoter in HindIII/EcoRI sites of pUC18. No 5'ss, No BS, No pseudo BSDepositorInsertRP51A (RPS17A Budding Yeast)
UseTagsExpressionBacterial and YeastMutation5'ss mut to C/ATACCT BS mut to GTTAGTG, pseu…PromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Prham-M.XbaI-M.EcoRI-M.SalI-p15A-aadA
Plasmid#170767PurposeMethylase expression vector for 5RM assembly: expresses M.XbaI, M.EcoRI and M.SalI DNA methyltransferases in E. coliDepositorInsertsM.XbaI
M.EcoRI
M.SalI
UseTagsExpressionBacterialMutationPromoterrhamnoseAvailable sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-CAAAG
Plasmid#170096PurposedeGFP reporter plasmid with CAAAG PAM upstream of p70a promoterDepositorInsertdeGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterp70aAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-CAATG
Plasmid#170097PurposedeGFP reporter plasmid with CAATG PAM upstream of p70a promoterDepositorInsertdeGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterp70aAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-GTAAT
Plasmid#170098PurposedeGFP reporter plasmid with GTAAT PAM upstream of p70a promoterDepositorInsertdeGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterp70aAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-GTATT
Plasmid#170099PurposedeGFP reporter plasmid with GTATT PAM upstream of p70a promoterDepositorInsertdeGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterp70aAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-PacI
Plasmid#170100PurposedeGFP reporter plasmid with unique PacI restriction enzyme recognition siteDepositorInsertdeGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterp70aAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEc-crRNA2
Plasmid#170089Purposeencodes E. coli type I-E single spacer arrayDepositorInsertE. coli type I-E single spacer array
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterJ23119Available sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-ATAAC
Plasmid#170095PurposedeGFP reporter plasmid with ATAAC PAM upstream of p70a promoterDepositorInsertdeGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterp70aAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH76
Plasmid#172144PurposeAccessory Plasmid for PACE of pylBCD variantsDepositorInsertPpsp gIII.2TAG, luxAB; Plpp pylS; PproK pylT
UseTagsExpressionBacterialMutationgIII(P29*, Y184*)PromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
VPS35 shRNA1
Plasmid#163858PurposeshRNA targeting the coding sequences of VPS35DepositorInsertVPS35 retromer complex component (VPS35 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
VPS35 shRNA2
Plasmid#163859PurposeshRNA targeting the coding sequences of VPS35DepositorInsertVPS35 retromer complex component (VPS35 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
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SAE2-mGarnet
Plasmid#175240PurposeBacterial expression and purification, combined with SAE1 to form the E1 enzyme heterodimerDepositorInsertUBA2 (UBA2 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
FKBP-EGFP-RanGAP
Plasmid#175236PurposeBacterial expression and purification, high affinity SUMOylation substrate that can recruited to FRB with rapamycinDepositorInsertRANGAP1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-RanGAP
Plasmid#175235PurposeBacterial expression and purification, high affinity SUMOylation substrateDepositorInsertRANGAP1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMbd3EosHaloTAF_Hyg
Plasmid#140958PurposeTargeting construct to make Mbd3-Eos-Halo-Tev-Avi-3xFlag fusionDepositorInsertmethyl-CpG binding protein 3 (Mbd3 Synthetic, Mouse)
UseMouse TargetingTags3xFLAG, Avi, Eos, Halo, and TEVExpressionMutationPromoterAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only