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pRetro-XT-PAK2-CFP(puro)-WT

Plasmid

#81176

Purpose

Retroviral vector for expression of wild-type PAK2 with a CFP fusion

Depositor

Angeliki Malliri

Article

Whalley et al Nat Commun. 2015 Jun 16;6:7437. doi: 10.

Insert

p21 (RAC1) activated kinase 2 (PAK2 Human)

Use

Retroviral

Tags

CFP

Expression

Mutation

Promoter

P-tight TRE

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only
pENTR-MBD1

Plasmid

#47057

Purpose

Expresses methyl-CpG binding domain protein 1

Depositor

Huda Zoghbi

Article

Sakai et al Sci Transl Med. 2011 Jun 8;3(86):86ra49.

Insert

Methyl-CpG Binding Domain Protein 1 (MBD1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only
mVenus-NES-SspB-RGL2-GEF-P2A-iLIDcaax

Plasmid

#173875

Purpose

Mammalian expression of mVenus-NES-SspB-RGL2-GEF-P2A-iLIDcaax (opto-Ral)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mVenus-NES-SspB-RGL2GEF-P2A-iLIDcaax

Use

Tags

C-terminal CAAX-box and mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-NES-SspB-RGL2-GEF-P2A-iLIDcaax

Plasmid

#173876

Purpose

Mammalian expression of mCherry-NES-SspB-RGL2-GEF-P2A-iLIDcaax (opto-Ral)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mCherry-NES-SspB-RGL2GEF-P2A-iLIDcaax

Use

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-161

Plasmid

#118541

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mTurquoise2

Use

Minitn5 delivery vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcihygroflagsynhDAT

Plasmid

#19990

Depositor

Jonathan Javitch

Article

Saunders et al Proc Natl Acad Sci U S A. 2000 Jun 6. 97

Insert

Dopamine transporter (SLC6A3 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2009

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Linear-2_PolyU(1)_Tornado-Corn

Plasmid

#159487

Purpose

Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-2_PolyU(1)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6-27

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-EGFP-mir30(Scn9a-scrambled)

Plasmid

#79671

Purpose

Scrambled control for Cre-dependent knockdown of Scn9a (Nav1.7)

Depositor

Scott Sternson

Article

Branco et al Cell. 2016 Jun 16;165(7):1749-61. doi: 1

Insert

EGFP

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pNICE HA-mSYD1B

Plasmid

#59362

Purpose

Expression plasmid for HA-tagged mSYD1B / Syde2 (HA-tag at N-terminus of mSYD1B)

Depositor

Peter Scheiffele

Article

Wentzel et al Neuron. 2013 Jun 19;78(6):1012-23. doi:

Insert

mSYD1B (Syde2 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-157

Plasmid

#118571

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mClover3

Use

Minitn7 delivery vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-156

Plasmid

#118538

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mCardinal

Use

Minitn5 delivery vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS-6015nes

Plasmid

#108657

Purpose

A S6 Kinase biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

Depositor

Michiyuki Matsuda

Article

Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

Insert

hyBRET-S6K-EV

Use

Tags

XFP

Expression

Mammalian

Mutation

Promoter

pCAGGS

Available since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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3349 pSFFV-neo Bcl-2

Plasmid

#8769

Depositor

Stanley Korsmeyer

Article

Yamamoto et al Mol Cell Biol 1999 Dec;19(12):8469-78.

Insert

Bcl-2 (BCL2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pCSIIbsr-6013x

Plasmid

#108654

Purpose

An HRas biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

Depositor

Michiyuki Matsuda

Article

Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

Insert

Raichu-EV

Use

Lentiviral

Tags

XFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-hSyn-Gαq*-BERKY1

Plasmid

#158430

Purpose

BRET biosensor for detection of Gαq-GTP in lentiviral vector pLenti-hSyn

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-YFP-GRK2(RH)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hSynapsin

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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CSII-IDR2-rhesusTRIM5-FOS

Plasmid

#79059

Purpose

Express rhesus TRIM5 in mammalian cells

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

rhesusTRIM5-FOS

Use

Tags

OneSTrEP-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2-HA-GAAZFP-FokI-RR

Plasmid

#18754

Depositor

Scot Wolfe

Article

Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

Insert

zinc finger nuclease

Use

Tags

Expression

Bacterial

Mutation

RNA expression vector for expressing the zinc fin…

Promoter

Available since

July 11, 2008

Availability

Academic Institutions and Nonprofits only

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pCAGGS-6017nes

Plasmid

#108655

Purpose

A PKA biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

Depositor

Michiyuki Matsuda

Article

Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

Insert

hyBRET-PKA-EV

Use

Tags

XFP

Expression

Mammalian

Mutation

Promoter

pCAGGS

Available since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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pRS405 PEX14-VAC8-RFP

Plasmid

#166844

Purpose

Expresses Peroxisome localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 390 base pairs from the PEX14 ORF

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8, PEX14 Budding Yeast)

Use

Tags

PEX14 transmembrane domain and RFP

Expression

Yeast

Mutation

PEX14 transmembrane domain

Promoter

VAC8 promoter

Available since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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S12.1_Empty_Bridge

Plasmid

#127670

Purpose

Ampicillin resistant multiplexing backbone without CRY2HA and mCherry

Depositor

Jennifer Phillips-Cremins

Article

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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