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pMTTH+eGFP-bromo1

Plasmid

#157805

Purpose

Expression of synthetic protein composed of eGFP and BRD4 bromodomain #1

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

eGFP-bromo1

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

pTAC

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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plc242-GFP-Nprl2

Plasmid

#87051

Purpose

mammalian expression of Nprl2

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

Nprl2 (NPRL2 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-LacI-p300

Plasmid

#179270

Purpose

Expresses GFP-LacI-p300 fusion plasmid

Depositor

Lienhard Schmitz

Article

Pfisterer et al Methods Mol Biol. 2023;2589:361-376. doi

Insert

E1A binding protein p300 (EP300 Human)

Use

Tags

GFP-LacI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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SUMO3_pcDNA6.2/EmGFP-Bsd

Plasmid

#176949

Purpose

Mammalian expression vector encoding SUMO3 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

SUMO3 (SUMO3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV Puro-pGK:GFP

Plasmid

#68486

Purpose

Retrovirus expressing GFP driven by pGK promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Puromycin Resistance
GFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR and pGK

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pTRE:iRFP670-EFS:Cre-2A-GFP

Plasmid

#68477

Purpose

Inducible lentivirus expressing iRFP670, Cre, and GFP

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

iRFP670
Cre-2A-GFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EFS and pTRE

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pPOTv7-g418-spGFP(11)

Plasmid

#190257

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Jack Sunter

Article

Paterou et al bioRxiv 2023.04.21.537815

Insert

spGFP(11)

Use

For endogenous gene tagging

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pME-GFP-P2A

Plasmid

#80807

Purpose

GFP without stop codon followed by porcine teschovirus-1 2A (P2A) "self-cleaving" peptide

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

GFP-P2A

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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GAL FLAG103QDProGFP p306

Plasmid

#15834

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 103Q Delta Pro (HTT Human)

Use

Tags

FLAG and GFP

Expression

Yeast

Mutation

Proline-rich region removed

Promoter

Available since

Oct. 5, 2007

Availability

Academic Institutions and Nonprofits only
pCN-HHT2-GFP

Plasmid

#45322

Depositor

Brendan Cormack

Article

Zordan et al G3 (Bethesda). 2013 Oct 3;3(10):1675-86.

Insert

yeGFP

Use

C. glabrata expression

Tags

Expression

Mutation

Promoter

HHT2

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pGFP Glra2 (CC#301)

Plasmid

#15199

Depositor

Connie Cepko

Article

Young et al Neuron. 2004 Mar 25. 41(6):867-79.

Insert

Glra2 (GLRA2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/GFP-GIGYF1

Plasmid

#141188

Purpose

Expresses GFP-GIGYF1 in mammalian cells, can be used to make inducible cell line

Depositor

Simon Bekker-Jensen

Article

Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

Insert

GIGYF1 (GIGYF1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-STX17ΔNTD

Plasmid

#89941

Purpose

Expresses syntaxin17 lacking N-terminal domain tagged with GFP in mammalian cells

Depositor

Noboru Mizushima

Article

Uematsu et al Autophagy. 2017 Aug 3;13(8):1452-1464. d

Insert

human syntaxin17 lacking N-terminal domain (STX17 Human)

Use

Retroviral

Tags

GFP

Expression

Mutation

deleted amino acids 1-161

Promoter

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-TIS11B CPM2

Plasmid

#121163

Purpose

Mammalian expression of GFP tagged TIS11B CPM2 (charge pattern mutant 2)

Depositor

Christine Mayr

Article

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Insert

ZFP36L1 (TIS11B) charge pattern mutant 2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pIBA3-SpyCatcher-sfGFP

Plasmid

#107420

Purpose

For production of SpyCatcher protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.

Depositor

Jack Leo

Article

Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/

Insert

SpyCatcher

Use

Tags

His tag, StrepII, and superfolder GFP

Expression

Bacterial

Mutation

Promoter

Tet

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-scrCdh2.1 GFP

Plasmid

#209100

Purpose

GFP expressing scramble of shRNA targeting Cdh2

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

scrCdh2.1

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-NDUFV2-mVenus

Plasmid

#185653

Purpose

NDUFV2 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFV2 (NDUFV2 Human)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Diffusive 27kDa (EGFP)

Plasmid

#201362

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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NET43 pEGFP-N2 (609)

Plasmid

#61991

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Insert

NET43 (NAT10 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP C1-Eps8 ∆cap

Plasmid

#74786

Purpose

mammalian expression of the capping activity mutant Eps8 fused to GFP

Depositor

Giorgio Scita

Article

Hertzog et al PLoS Biol. 2010 Jun 1;8(6):e1000387. doi

Insert

Eps8 (Eps8 Mouse)

Use

Tags

Expression

Mammalian

Mutation

V689D, L693D

Promoter

Available since

May 3, 2016

Availability

Academic Institutions and Nonprofits only