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Plasmid#54620PurposeLocalization: N1 Cloning Vector, Excitation: 683, Emission: 704DepositorTypeEmpty backboneUseTagsmIFPExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits
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pCW57.1-Luciferase
Plasmid#99283PurposeAll-in-one lentivector with tet-inducible luciferaseDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
RAD21 C-tag CRISPR
Plasmid#140540PurposeRAD21 tagging CRISPRDepositorInsertRAD21 (RAD21 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_WNK3_p.V488A
Plasmid#81723PurposeGateway Donor vector containing WNK3, part of the Target Accelerator Plasmid Collection.DepositorInsertWNK3 (WNK3 Human)
UseGateway entry vectorTagsExpressionMutationV488APromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOpen-DpnIR
Plasmid#165529PurposeUsed to digest methylated DNA; to be expressed in dam- E. coli strains (strains which do not methylate their genomic DNA)DepositorInsertDpnI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEvolvR-enCas9-PolI5M
Plasmid#113078PurposeExpresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.DepositorInsertenCas9-PolI5M
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNCS BFP
Plasmid#91757PurposeBacterial expression of a fluorescent protein, Blue Fluorescent Protein (BFP)DepositorInsertBlue Fluorescent Protein
UseTagsHexa-Histidine, Xpress epitope for detection with…ExpressionBacterialMutationGFP (Y66H)PromoterT7Available sinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP Snail WT
Plasmid#16225DepositorInsertSnail (SNAI1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-mEGFP-HMGB1-WT
Plasmid#194543PurposeBacterial Expression of 6xHis mEGFP-HMGB1 WT Fusion ProteinDepositorInsertHMGB1 (HMGB1 Human)
UseTagsmEGFPExpressionBacterialMutationPromoterAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
PCR4-Hsp68::mCherry-SI-Hsp68::eGFP-H11
Plasmid#211942Purposedual-enSERT-2.2 vector for site-specific integration of.a two-color enhancer-reporter construct into the H11 locus (separated by a synthetic insulator)DepositorTypeEmpty backboneUseCRISPR and Mouse TargetingTagsExpressionMammalianMutationPromoterHSP68Available sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pmGFP
Plasmid#117926PurposeControl plasmid expressing myc-GFP.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmRuby3-24xGCN4
Plasmid#132413Purposeoverexpression in human cellsDepositorInsertmRuby3
UseTagsExpressionMammalianMutationnoPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-pCbh-ABE8e-nSpCas9(D10A/A61R)[N-term] (CA19)
Plasmid#197510PurposeCbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A/A61R) (to be used with SpRY C-term constructs)DepositorInsertAAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A/A61R)[N-term]-NpuN-BPNLS-[ITR]
UseAAV and CRISPRTagsBPNLS and NpuN(intein)-BPNLSExpressionMutationABE8e mutations in TadA and nSpRY(D10A/A61R)PromoterCbhAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-NR2F2
Plasmid#192900PurposeBarcoded piggybac transposon vector with Dox-inducible expression of NR2F2DepositorInsertNR2F2 (NR2F2 Human)
UseTagsExpressionMammalianMutationPromoterTET3G-miniCMVAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-TRE3G-N-tdT-CMV-P
Plasmid#192887PurposeTet-inducible Nanoluc-tdTomato expressionDepositorInsertNanoluc-P2A-tdTomato
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXL010-Wnt dual (GFP-Fire) reporter
Plasmid#40588DepositorInsertWnt reporter sequences
UseLentiviralTagsExpressionMammalianMutationPromoterWnt promoterAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPathBrick
Plasmid#65006PurposeE. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricksDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterConstitutive native promotersAvailable sinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
TFORF2885
Plasmid#144256PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertGLI2 (GLI2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CBh-mKate2-IRES-MCS (WT-WT)
Plasmid#105921PurposeAAV-mediated gene expression from CBh promoter. Contains canonical left and right AAV2 ITRsDepositorHas ServiceAAV2InsertsmKate2
IRES-Multiple cloning site
UseAAVTagsExpressionMutationcodon optimizedPromoterCBh and same CBh promoter as mKate2Available sinceFeb. 15, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
Fuw-dCas9-Dnmt3a
Plasmid#84476PurposeLentiviral construct to express dCas9 fused with Dnmt3aDepositorInsertdCas9-Dnmt3a (DNMT3A Synthetic, Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR v2-sgLKB1 clone2
Plasmid#162126PurposeLentiviral sgRNA plasmid targeting human LKB1DepositorInsertsgLKB1 (STK11 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SFFV-NFE2L1-Puro
Plasmid#181917PurposeLentiviral plasmid that directs the expression of NFE2L1/Nrf1 in mammalian cells.DepositorInsertNFE2L1 (NFE2L1 Human)
UseLentiviralTagsFLAG and HAExpressionMammalianMutationPromoterSFFVAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pR26
Plasmid#127372PurposeAllows for doxycycline-inducible gene expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.DepositorTypeEmpty backboneUseCRISPR and Mouse TargetingTagsExpressionMutationPromotertight TRE promoterAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
PL-SIN-Oct4-EGFP
Plasmid#21319PurposeLentiviral vector, expresses EGFP under Oct3/4 promoterDepositorInsertEnhanced Green Fluorescence Protein
UseLentiviralTagsExpressionMammalianMutationPromoterOct-4 promoter regionAvailable sinceJune 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 EYFP H148Q/I152L
Plasmid#25872DepositorInsertEYFP H148Q/I152L
UseTagsExpressionMammalianMutationEYFP from pEYFP (Clontech) with H148Q and I152L m…PromoterAvailable sinceAug. 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
BS-Ura3Kl
Plasmid#69195Purposecomplementation of yeast auxotrophic marker URA3(Kluyveromyces lactis)DepositorInsertKluyveromyces lactis URA3
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLF3FShC
Plasmid#153083PurposeRMCE integration vector with self excising cassetteDepositorTypeEmpty backboneUseRecombination mediated cassette exchangeTagsExpressionWormMutationPromoterAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYLCRISPR/Cas9Pubi-H
Plasmid#66187Purposeexpression of Cas9 in plants, maize ubiquitin promoter (Pubi), hygro selectionDepositorInsertCas9
UseTagsExpressionPlantMutationplant-codon optimized with higher GC contents (62…Promotermaize ubiquitin promoter (Pubi)Available sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
p5E--3mnx1
Plasmid#74632PurposeCan be used to drive expression specifically in zebrafish motor neuronsDepositorInsertmotor neuron and pancreas homeobox 1 promoter (mnx1 Zebrafish)
Use5' entry vector for multisite gateway three …TagsExpressionMutationPromoter-3mnx1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHN781
Plasmid#178828PurposeBacterial expression for human Saposin ADepositorInsertartificial gene encoding human Saposin A (AR Human)
UseTagsHis8, TEVExpressionBacterialMutationCodon-usage was optimized for bacterial expressio…PromoterT7Available sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-TLR6
Plasmid#118997PurposeTargeting vector for human AAVS1 with TLR6 reporter insertDepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET30a-AlkB-D135S
Plasmid#79051PurposeExpress mutant AlkB protein (D135S) in E. coli BL21(DE3)DepositorInsertAlkB (alkB E.coli)
UseTagsHis tagExpressionBacterialMutationA truncated AlkB with deletion of the amino (N)-t…PromoterT7Available sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
MXS_BidirectionalCAG
Plasmid#78175PurposeBidirectional CAG promoter (compatible with the MXS Chaining Kit)DepositorInsertBidirectional CAG Promoter
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNCS-CyOFP
Plasmid#74278PurposeConstitutive expression of CyOFP in bacteriaDepositorInsertCyOFP
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGG-B1
Plasmid#78504PurposeTrans plasmid for production of recombinant AAV-B1 vectors. Plasmid backbone may yield higher titers than pAR-B1 and encodes AAV2 Rep and AAV-B1 capsidDepositorInsertsAAV2 Rep
AAV-B1 capsid
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G4-Km
Plasmid#46819PurposeTEF1 and HXT7 promoter controlled expression cassettes with G418 resistance for yeast transformationDepositorTypeEmpty backboneUseTagsFLAG and c-mycExpressionYeastMutationPromoterTEF1 and HXT7Available sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRBC-sfGFP-150TAG
Plasmid#174076PurposePlasmid with p15a origin of replication expressing codon optimized superfolder GFP-150 TAG with C-terminal His6 tag, under T7 promoter,DepositorInsertSuperfolder GFP
UseTagsHis-6ExpressionBacterialMutationN150TAGPromoterT7Available sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
N-cadherin in pCCL-c-MNDU3c-PGK-EGFP
Plasmid#38153DepositorInsertsUseLentiviralTagsExpressionMutationPromoterMNDU3c = LTR promoter for U3 region of myeloproli…Available sinceAug. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1 METTL3
Plasmid#200731PurposeBacterial expression of GST-Tagged codon optimized human METTL3DepositorInsertMETTL3 (METTL3 Human)
UseTagsGST from backbone plasmidExpressionBacterialMutationCodon optimized for expression in E. coliPromoterAvailable sinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only