Addgene

We narrowed to 15,718 results for: puromycin

Showing: 5041 - 5060 of 15718 results

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pX459-dNrg1

Plasmid

#173696

Purpose

A knockout vector for the dog Nrg1.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Nrg1 gene and the cDNA of CRISPR-Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pX459-dEreg

Plasmid

#173697

Purpose

A knockout vector for the dog Ereg.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Ereg gene and the cDNA of CRISPR-Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pX459-dTgfa

Plasmid

#173698

Purpose

A knockout vector for the dog Tgfa.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Tgfa gene and the cDNA of CRISPR-Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pX459-dHbegf

Plasmid

#173699

Purpose

A knockout vector for the dog Hbegf.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Hbegf gene and the cDNA of CRISPR-Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2-puro-dHbegf

Plasmid

#173841

Purpose

A knockout vector for the dog Hbegf.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Hbegf gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPBpuro-UbC-canisHbegf

Plasmid

#173846

Purpose

Encoding dog Hbegf.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A cDNA of canis Hbegf.

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPBpuro-UbC-canisTgfa

Plasmid

#173847

Purpose

Encoding dog Tgfa.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A cDNA of canis Tgfa.

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPBpuro-UbC-canisEreg

Plasmid

#173848

Purpose

Encoding dog Ereg.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A cDNA of canis Tgfa.

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pmx-puro-Mef2c-i

Plasmid

#172398

Purpose

Retroviral expression vector for murine direct cardiac reprogramming

Depositor

Li Qian

Article

Wang et al Cells. 2020 Jan 22;9(2). pii: cells90202

Insert

Mef2c-i (Mef2c Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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Clim-DC2-lifeact-mCherry

Plasmid

#129558

Purpose

pClimDCLifeAct:mCh actin filaments marker

Depositor

Inaki Ruiz-Trillo

Article

Kozyczkowska et al Curr Biol. 2021 Sep 27;31(18):4104-4110.

Insert

lifeAct fused to mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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p8195 MSCV-P C-FlagHA 6E7 R77S

Plasmid

#163309

Purpose

Expresses HPV6 E7 R77S

Depositor

Elizabeth White

Article

Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

Insert

HPV6 E7 R77S

Use

Retroviral

Tags

FlagHA

Expression

Mutation

R77S

Promoter

MSCV LTR

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgNADK_1

Plasmid

#163462

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting NADK

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

sgRNA 1 targeting NADK (NADK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLJC2-GPT1-3xFLAG

Plasmid

#163446

Purpose

lentiviral overexpression vector for human GPT1

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

GPT1 (GPT Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

See: comments

Promoter

CMV

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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p8194 MSCV-P C-FlagHA 31E7 R77S

Plasmid

#163308

Purpose

Expresses HPV31 E7 R77S

Depositor

Elizabeth White

Article

Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

Insert

HPV31 E7 R77S

Use

Retroviral

Tags

FlagHA

Expression

Mutation

R77S

Promoter

MSCV LTR

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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p8116 LentiCRISPR v2 sgPTPN14-4

Plasmid

#163316

Purpose

Expresses Cas9 and a human PTPN14-targeting control guide RNA

Depositor

Elizabeth White

Article

Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

Insert

sgPTPN14-4

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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p8193 MSCV-P C-FlagHA 18E7 R84S

Plasmid

#163307

Purpose

Expresses HPV18 E7 R84S

Depositor

Elizabeth White

Article

Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

Insert

HPV18 E7 R84S

Use

Retroviral

Tags

FlagHA

Expression

Mutation

R84S

Promoter

MSCV LTR

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pEBTetD-SNAP-POC5

Plasmid

#136787

Purpose

Mammalian expression of the centrosomal protein POC5 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-POC5 (POC5 Human, Synthetic)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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BII-C3H2B

Plasmid

#133387

Purpose

Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

Depositor

Blair Madison

Article

Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV enhancer and hEf1a (CpG free)

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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BII-gR-PnGW

Plasmid

#133355

Purpose

Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications), co-expressed with GFP-NLS

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

GFP-NLS

Use

Tags

HA-tagged GFP

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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PLK4 G8.1 gRNA

Plasmid

#90837

Purpose

3rd generation lentiviral gRNA plasmid targeting human PLK4

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PLK4 (Guide Designation G8.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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