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MAC-C Sars-CoV-2 ORF8_R52I

Plasmid

#194827

Purpose

Sars-CoV-2 ORF8_R52I in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

ORF8_R52I (ORF8 Sars-CoV-2)

Use

Tags

MAC-C

Expression

Mammalian

Mutation

R52I

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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HA-C Sars-CoV-2 E P71L

Plasmid

#194804

Purpose

Sars-CoV-2 E_P71L in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

E_P71L (E Sars-CoV-2)

Use

Tags

MAC-C

Expression

Mammalian

Mutation

P71L

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 N_D3L

Plasmid

#194806

Purpose

Sars-CoV-2 N_D3L in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

N_N_D3L (N Sars-CoV-2)

Use

Tags

MAC-C

Expression

Mammalian

Mutation

N_D3L

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 N_D63G

Plasmid

#194807

Purpose

Sars-CoV-2 N_D63G in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

N_D63G (N Sars-CoV-2)

Use

Tags

MAC-C

Expression

Mammalian

Mutation

D63G

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 N_R203K+G204R

Plasmid

#194809

Purpose

Sars-CoV-2 N_R203K+G204R in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

N_R203K+G204R (N Sars-CoV-2)

Use

Tags

MAC-C

Expression

Mammalian

Mutation

R203K+G204R

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 N_R203M

Plasmid

#194810

Purpose

Sars-CoV-2 N_R203M in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

N_R203M (N Sars-CoV-2)

Use

Tags

MAC-C

Expression

Mammalian

Mutation

R203M

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 N_S235F

Plasmid

#194811

Purpose

Sars-CoV-2 N_S235F in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

N_S235F (N Sars-CoV-2)

Use

Tags

MAC-C

Expression

Mammalian

Mutation

S235F

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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HA-C Sars-CoV-2 N_T205I

Plasmid

#194812

Purpose

Sars-CoV-2 N_T205I in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

N_T205I (N Sars-CoV-2)

Use

Tags

MAC-C

Expression

Mammalian

Mutation

T205I

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 NSP12_P314L

Plasmid

#194813

Purpose

Sars-CoV-2 NSP12_P314L in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

NSP12_P314L (ORF1ab Sars-CoV-2)

Use

Tags

MAC-C

Expression

Mammalian

Mutation

P314L

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 NSP13_E341D

Plasmid

#194814

Purpose

Sars-CoV-2 NSP13_E341D in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

NSP13_E341D (ORF1ab Sars-CoV-2)

Use

Tags

MAC-C

Expression

Mammalian

Mutation

E341D

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 NSP13_P77L

Plasmid

#194815

Purpose

Sars-CoV-2 NSP13_P77L in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

NSP13_P77L (ORF1ab Sars-CoV-2)

Use

Tags

MAC-C

Expression

Mammalian

Mutation

P77L

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 NSP2_T85I

Plasmid

#194816

Purpose

Sars-CoV-2 NSP2_T85I in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

NSP2_T85I (ORF1ab Sars-CoV-2)

Use

Tags

MAC-C

Expression

Mammalian

Mutation

T85I

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 NSP3_A890D

Plasmid

#194817

Purpose

Sars-CoV-2 NSP3_A890D in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

NSP3_A890D (ORF1ab Sars-CoV-2)

Use

Tags

MAC-C

Expression

Mammalian

Mutation

A890D

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 NSP3_K977Q

Plasmid

#194818

Purpose

Sars-CoV-2 NSP3_K977Q in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

NSP3_K977Q (ORF1ab Sars-CoV-2)

Use

Tags

MAC-C

Expression

Mammalian

Mutation

K977Q

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-CMV-BD10-SAS620-3’dCas9-VPR-synpA

Plasmid

#216323

Purpose

Transactivation of murine Myo7b gene via split dCas9-VPR (REVeRT system).

Depositor

Elvir Becirovic

Article

Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

Insert

Split Cas9-VPR + splice acceptor site

Use

AAV

Tags

Expression

Mutation

Promoter

CMV

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-3xsgMyo7b-CMV-5’dCas9-SDS-BD10-SV40pA

Plasmid

#216322

Purpose

Transactivation of murine Myo7b gene via split dCas9-VPR (REVeRT system).

Depositor

Elvir Becirovic

Article

Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

Insert

Split Cas9-VPR + splice donor site + gRNAs targeting the murine Myo7b locus for transactivation

Use

AAV

Tags

Expression

Mutation

Promoter

CMV

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-5'Luciferase(split CAGGT)_BDlacZ-SV40polyA

Plasmid

#216320

Purpose

Split luciferase assay to test reconstitution via mRNA trans-splicing (REVeRT system).

Depositor

Elvir Becirovic

Article

Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

Insert

Split Luciferase + splice donor site

Use

AAV

Tags

Expression

Mutation

Promoter

CMV

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV2.1-CMV-BD lacZ_3' Cer-bGHpA

Plasmid

#216319

Purpose

Split fluorophore assay to test reconstitution via mRNA trans-splicing (REVeRT system).

Depositor

Elvir Becirovic

Article

Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

Insert

Split Cerulean + splice acceptor site

Use

AAV

Tags

Expression

Mutation

Promoter

CMV

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV2.1-CMV-5'Cer-BDlacZ-bGHpA

Plasmid

#216318

Purpose

Split fluorophore assay to test reconstitution via mRNA trans-splicing (REVeRT system).

Depositor

Elvir Becirovic

Article

Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

Insert

split cerulean fluorescent protein + splice donor site

Use

AAV

Tags

Expression

Mutation

Promoter

CMV

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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METTL3

Plasmid

#155725

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

METTL3 (METTL3 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

H330Q compared to BC052244, no mutations compared…

Promoter

EF-1a

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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