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Plasmid#166465PurposeExpresses fusion of mEGFP and YAPS127A- L318EDepositorInsertmEGFP, YAPS127A- L318E (YAP1 Human)
UseTagsExpressionMammalianMutationYAPS127A- L318EPromoterAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
MBP-rPKL WT
Plasmid#107156PurposeMBP-rPKL WT for production and purification of rat PKL WTDepositorInsertMBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
UseTagsC-term 6-his and N-term MBPExpressionBacterialMutationPromoterPBADAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag-hPKL S113D
Plasmid#107161PurposeFor mammalian expression of Flag-hPKL S113DDepositorInsertFlag-hPKL S113D (PKLR Human)
UseRetroviralTagsN-term FlagExpressionMammalianMutationSerine 113 mutated to aspartatePromoterCMVAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag-hPKL S113A
Plasmid#107160PurposeFor mammalian expression of Flag-hPKL S113ADepositorInsertFlag-hPKL S113A (PKLR Human)
UseRetroviralTagsN-term FlagExpressionMammalianMutationSerine 113 mutated to alaninePromoterCMVAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pet-ECHB(35-475)
Plasmid#96890Purposeexpresses His-tagged mouse ECHB (35-475) in E.coli cellsDepositorInsertTrifunctional enzyme subunit beta, mitochondrial (Hadhb Mouse)
UseTagsHis6 tagExpressionBacterialMutationdeleted amino acid 1-34PromoterT7Available sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
LZF40: LZF40_Fsyn_Cre
Plasmid#109378PurposeSynaptic transmissionDepositorInsertCre-NCBI
UseLentiviralTagsExpressionMammalianMutationPromoterhuman synapsin IAvailable sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
sgALK(Chr. Inv.)
Plasmid#85569PurposeExpresses sgRNA targeting human ALK between exon 20 and 21DepositorInsertanaplastic lymphoma receptor tyrosine kinase (ALK Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJJR50
Plasmid#75026PurposeU6 promoter driven flipped + extended sgRNA expression vectorDepositorInsertguide RNA, flipped and extended version
UseCRISPRTagsExpressionMutationPromoterR07E5.16 (U6)Available sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVZ_PcoaT_SQE1
Plasmid#103860PurposeHeterologous, cobalt-inducible expression of SQE1 from A. thaliana in Synechocystis sp. PCC6803DepositorInsertsqualene epoxidase 1 (XF1 Mustard Weed)
UseSynthetic BiologyTagsExpressionBacterialMutationinserted gene is codon optimized. 2nd amino acid …PromoterPcoaT (from Synechocystis sp. PCC 6803)Available sinceJan. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet-ECHA(37-763)-FLAG (no His-tag)
Plasmid#96887Purposeexpresses flag-tagged mouse ECHA (37-768) in E.coli cells (no His-tag)DepositorInsertTrifunctional enzyme subunit alpha, mitochondrial (Hadha Mouse)
UseTagsFLAG-taggedExpressionBacterialMutationdeleted amino acid 1-36PromoterT7Available sinceJuly 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDF-casBCDE(+6)
Plasmid#89728PurposeExpresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a longer version of the wt spacer (+6) extended by 6 nucleotides at the leader-distal end.DepositorInsertCRISPR array
UseTagsExpressionBacterialMutationDerivative CRISPR array containing a longer versi…PromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
3' MBD3 TALEN
Plasmid#52359PurposeTALEN vector for targeting human MBD3 locus (3')DepositorInsertNN NN HD NN NG NN NI HD HD HD HD NI NN NI HD HD NG
UseTALENTagsExpressionMutationPromoterAvailable sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET20b-mPTER
Plasmid#222189PurposeBacterial expression of mouse PTER proteinDepositorInsertPTER (Pter Mouse)
UseTags6xHis and StrepExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-TEX264-SIM1_mut-cFlag
Plasmid#220205PurposeMammalian expression of TEX264 with SIM1 motif mutated, C-terminal FlagDepositorInsertTEX264 (TEX264 Human)
UseTagsFlagExpressionMammalianMutationp.111LIDLY>AAAAA; c.331C>G,332T>C,334A&g…PromoterCMVAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5-TEX264-SIM1_mut-cGFP
Plasmid#220212PurposeMammalian expression of TEX264 with SIM1 motif mutated, fused at C-terminus to GFPDepositorInsertTEX264 (TEX264 Human)
UseTagsGFPExpressionMammalianMutationp.111LIDLY>AAAAA; c.331C>G,332T>C,334A&g…PromoterCMV/TetOAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
MAC-C Sars-CoV-2 NSP5_K90R
Plasmid#194819PurposeSars-CoV-2 NSP5_K90R in MAC-C destination vectorDepositorInsertNSP5_K90R (ORF1ab Sars-CoV-2)
UseTagsMAC-CExpressionMammalianMutationK90RPromoterAvailable sinceMay 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC-C Sars-CoV-2 NSP6_S107-109del
Plasmid#194820PurposeSars-CoV-2 NSP6_S107-109del in MAC-C destination vectorDepositorInsertNSP6_S107-109del (ORF1ab Sars-CoV-2)
UseTagsMAC-CExpressionMammalianMutationS107-109delPromoterAvailable sinceMay 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC-C Sars-CoV-2 ORF3a_Q57H
Plasmid#194821PurposeSars-CoV-2 ORF3a_Q57H in MAC-C destination vectorDepositorInsertORF3a_Q57H (ORF3a Sars-CoV-2)
UseTagsMAC-CExpressionMammalianMutationQ57HPromoterAvailable sinceMay 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC-C Sars-CoV-2 ORF7a_T120I
Plasmid#194823PurposeSars-CoV-2 ORF7a_T120I in MAC-C destination vectorDepositorInsertORF7a_T120I (ORF7a Sars-CoV-2)
UseTagsMAC-CExpressionMammalianMutationT120IPromoterAvailable sinceMay 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC-C Sars-CoV-2 ORF7a_V82A
Plasmid#194824PurposeSars-CoV-2 ORF7a_V82A in MAC-C destination vectorDepositorInsertORF7a_V82A (ORF7a Sars-CoV-2)
UseTagsMAC-CExpressionMammalianMutationV82APromoterAvailable sinceMay 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits