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Plasmid#105320PurposeMammalian expression of ultrasensitive protein calcium sensorDepositorInsertjGCaMP7c variant 1513
UseTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJBEI-6409
Plasmid#47048PurposeBglBrick plasmid (=pBbA5c-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coliDepositorInsertCodon-optimized sequences for MEV pathway expression in E. coli to produce limonene
UseSynthetic Biology; BglbrickTagsExpressionBacterialMutationPromoterPlacUV5Available sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 MYC-Sumo3 WT
Plasmid#48964PurposeExpresses MYC-Sumo3 Wild Type in mammalian cellsDepositorInsertSUMO3 (SUMO3 Human)
UseTagsMYC (EQKLISEED)ExpressionMammalianMutationPromoterT7Available sinceNov. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet_alphaTubulin_C1
Plasmid#85045PurposeIn vivo visualization of microtubules (can be used for colocalization studies)DepositorInsertAlpha-Tubulin (TUBA1B Human)
UseTagsmScarletExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
mOrange-C1
Plasmid#54680PurposeLocalization: C1 Cloning Vector, Excitation: 548, Emission: 562DepositorTypeEmpty backboneUseTagsmOrangeExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
p4xSTAT6-Luc2P
Plasmid#35554DepositorInsert4xStat6 promoter
UseLuciferaseTagsExpressionMammalianMutationPromoter4xStat6Available sinceMarch 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLenti.CAG.H2B-Dendra2.W
Plasmid#51005PurposeLentivector that expresses H2B-Dendra2 from internal CAG enhancer-promoterDepositorInsertH2B-Dendra2
UseLentiviralTagshistone2BExpressionMammalianMutationPromoterCAGAvailable sinceJune 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBlu/gRNA
Plasmid#59188PurposeServes as a shuttle vector for target oligo before insertion into Cas9 destination vectorDepositorInsertGuide RNA Cassette
UseCRISPRTagsExpressionPlantMutationPromoterU6 (Arabidopis)Available sinceFeb. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
C1A-C1A-EYFP
Plasmid#61155PurposeDiacylglycerol (DAG) reporterDepositorInsertC1A-C1A-EYFP, C1A from PKCG (aa 26-89, rat) (Prkcg Rat)
UseTagsEYFPExpressionMammalianMutationC1A from PKCG amino acids 26-89PromoterCMVAvailable sinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
C1(1-29)-miniTurbo-V5_pCDNA3
Plasmid#107174Purposeexpresses V5-tagged miniTurbo on the mammalian ER membraneDepositorInsertminiTurbo (BirA mutant)
UseTagsExpressionMammalianMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmTurquoise2-Mito
Plasmid#36208PurposeIn vivo visualization of the mitochondria (can be used for colocalization studies)DepositorInsertMTS (COX8A Human)
UseTagsmTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-vhhGFP4-hIgG1-Fc
Plasmid#105576PurposeT7 promotor drives in vitro mRNA transcription of GFP nanobody-Fc antibody domain fusionDepositorInsertHuman IgG1 Fc-domain fused with GFP nanobody (IGHG1 Human)
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFC333
Plasmid#87844PurposeAMA1 plasmid with Aspergillus optimized Cas9 and ble selection markerDepositorInsertsCas9
ble (bleomycin resistance marker)
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterAspergillus nidulans tef1 promoter and Aspergillu…Available sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-smURFP-IRES-eGFP
Plasmid#80343PurposeExpresses smURFP and eGFP in mammalian cells.DepositorInsertssmURFP
eGFP
UseTagsExpressionMammalianMutationPromoterCMV and IRESAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQE-80L MBP-SspB Nano
Plasmid#60409PurposeE. coli expression of SspB NanoDepositorInsertSspB nano
UseTagsHis-MBP-TEVExpressionBacterialMutationY11K and A15EPromoterAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE1
Plasmid#73019PurposeExpresses BE1 with C-terminal NLS in mammalian cellsDepositorInsertBE1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pARE-lux
Plasmid#11768DepositorInsertARE
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceMay 12, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDIRE
Plasmid#26745DepositorInsertiCre expression cassette
UseCre/Lox; Flp/frtTagsExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEP4 E02S EN2K
Plasmid#20925Purposeused in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Nanog and Klf4DepositorArticleUseEpisomal/ebnaTagsExpressionMutationPromoterAvailable sinceMay 20, 2009AvailabilityAcademic Institutions and Nonprofits only -
pC0040-LwaCas13a crRNA backbone
Plasmid#103851PurposeBackbone for cloning guides that are compatible with LwaCas13a. Contains a 5' direct repeat. Clone using BbsI (BpiI). F overhang AAAC. R overhang AAAADepositorInsertU6-LwaCas13aDR-BbsI-BbsI-polyT
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only