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Showing: 5001 - 5020 of 49746 results

  1. pAM5411

    Plasmid
    #132663
    Purpose
    RSF1010-based broad host-range plasmid with RK2 bom site and pUC origin expressing YFP and Sp/Sm resistance
    Depositor
    Susan Golden
    Article
    Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1
    Inserts
    aadA
    YFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PaadA and PconII
    Available since
    Nov. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    132663-plasmid-map-sequence-id-260955

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  3. AAV-SYN-NLS-SaCas9-NLS-3xHA-bGHpA-U6-BsaI-sgRNA

    Plasmid
    #218710
    Purpose
    AAV vector plasmid expressing Cas9 from Staphylococcus aureus (SaCas9) under the human synapsin (SYN) promoter and its sgRNA under the U6 promoter
    Depositor
    Bart Nieuwenhuis
    Inserts
    hSaCas9
    Chimeric guide for SaCas9
    Use
    AAV and CRISPR
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218710-plasmid-map-sequence-id-433357

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  4. p-dCas9-HDAC1-Hygro

    Plasmid
    #104409
    Purpose
    transient expression of dCas9-HDAC1 fusion protein
    Depositor
    Stephan Beck
    Insert
    HDAC1 (HDAC1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104409-plasmid-map-sequence-id-204471

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  6. 6xO/S luc

    Plasmid
    #69445
    Purpose
    detection/assessment of OCT4 and SOX2 transcription factor activity
    Depositor
    Lisa Dailey
    Insert
    minimal fgf4 promoter and 6XOS tandem repeat of composite OCT4/Sox2 binding site from FGF4 enhancer
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    69445-plasmid-map-sequence-id-259015

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  8. pGBW-m4046203

    Plasmid
    #145488
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Use
    Tags
    Cleavable thrombin;3xFLAG
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pGAL1
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145488-plasmid-map-sequence-id-306276

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  9. pJAT21

    Plasmid
    #204309
    Purpose
    For generating targeting inversions. N-terminal split GFP, expressed in abdomen and mouthparts
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    ie1-GFPn
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ie1
    Available since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    204309-plasmid-map-sequence-id-405852

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  10. pJAT25

    Plasmid
    #204310
    Purpose
    For generating targeting inversions. C-terminal split GFP, expressed in abdomen and mouthparts.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    ie1-GFPc
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ie1
    Available since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    204310-plasmid-map-sequence-id-405854

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  11. pGBW-m4046233

    Plasmid
    #149032
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 nsp3
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 nsp3 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Use
    Tags
    Cleavable thrombin;6xHIS
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pTEF1
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149032-plasmid-map-sequence-id-298060

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  12. pGBW-m4046664

    Plasmid
    #149224
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 E (envelope)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pTEF1
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149224-plasmid-map-sequence-id-291677

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  14. pRN1120

    Plasmid
    #101750
    Purpose
    Multi-copy (2 micron) yeast plasmid containing a functional NatMX marker cassette conferring resistance against nourseothricin. Recipient plasmid for sgRNA or crRNA expression cassettes.
    Depositor
    Rene Verwaal
    Article
    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Dec. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    101750-plasmid-map-sequence-id-199900

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  15. COVID-SARS2 NSP16/10

    Plasmid
    #159615
    Purpose
    Bacterial co-expression vector fo Covid-SARS2 NSP16 and NSP10
    Depositor
    Opher Gileadi
    Inserts
    Covid-SARS2 Nsp16
    Covid-SARS2 Nsp10
    Use
    Tags
    His6-Zb-TEV and none
    Expression
    Bacterial
    Mutation
    Codon-optimized for E. coli expression
    Promoter
    (Bicistronic) and T7 - LacO
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    159615-plasmid-map-sequence-id-311066

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  16. pJAT27

    Plasmid
    #204308
    Purpose
    For generating targeting inversions. C-terminal split GFP, expressed in flight muscles.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    MHC-GFPc
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    MHC
    Available since
    Oct. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    204308-plasmid-map-sequence-id-411129

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  17. pJAT18

    Plasmid
    #204307
    Purpose
    For generating targeting inversions. N-terminal split GFP, expressed in flight muscles.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    MHC-GFPn
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    MHC
    Available since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    204307-plasmid-map-sequence-id-405848

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  18. pGBW-m4134100

    Plasmid
    #152050
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;6xHis-003
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    May 20, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152050-plasmid-map-sequence-id-300237

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Showing: 5001 - 5020 of 49746 results