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Plasmid#123109PurposeExpresses 3xFLAG-LC3B2-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3B2DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)
UseTags3xFLAG and EGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC22A3
Plasmid#132024PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC22A3 (SLC22A3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBEG R2-L3 #BGV193
Plasmid#48952PurposeFor inserting selection gene between AttR2 and AttL3 sites for use in Gateway recombination cloning system (corresponds to a module 3 plasmid)DepositorTypeEmpty backboneUseGateway cloning vectorTagsExpressionMutationPromoterAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
JC101: pMVP/Ad/PK-DEST
Plasmid#121845PurposepMVP destination vector, empty pK fiber-modified adenovirus vector backboneDepositorTypeEmpty backboneUseAdenoviral; Pmvp gateway destination vectorTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
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p3E-CaaX(tH)
Plasmid#109539PurposeMultisite gateway vector for 3' tagging with the CaaX domain from HrasDepositorInsertCaaX domain from Hras
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-rab5aa
Plasmid#109716PurposeMultisite gateway vector for 3' tagging with rab5aaDepositorInsertrab5aa (rab5aa Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A-3xFlag-M694V-MEFV
Plasmid#167118PurposeGateway entry plasmid with 3xFlag-pyrin (M694V/FMF variant)DepositorInsertMEFV (MEFV Human)
UseGateway entry vectorTags3xFlagExpressionMutationM694V Methionine 694 to valine (FMF mutation)PromoterAvailable sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pME-Trpa1b-2A-mCherry
Plasmid#106291PurposeGateway middle entry construct containing zebrafish Trpa1b, 2A self-cleaving peptide and mCherryDepositorInsertTrpa1b (trpa1b Zebrafish)
UseGateway middle entryTags2A-mCherryExpressionMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-BirA(R118G)-YFP-NLS-STOP-L2
Plasmid#127360Purposegateway entry vector for expressing nuclear BirA* (R118G)-YFP under a promoter of choiceDepositorInsertBirA* (R118G mutant)
UseGateway entry vectorTagsGS linker, NLS, V5, and YFPExpressionMutationR118GPromoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pDEST-actc1b-ccdb-mKate2
Plasmid#109731PurposeMultisite gateway destination vector for muscle specific expression of constructs tagged at the 3' end with mKate2DepositorTypeEmpty backboneUseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST-actc1b-mKate2-ccdb
Plasmid#109732PurposeMultisite gateway destination vector for muscle specific expression of constructs tagged at the 5' end with mKate2DepositorTypeEmpty backboneUseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-BirA(R118G)-NES-YFP-STOP-L2
Plasmid#127357Purposegateway entry vector for expressing cytosolic BirA* (R118G)-YFP under a promoter of choiceDepositorInsertBirA* (R118G mutant)
UseGateway entry vectorTagsGS linker, NES, V5, and YFPExpressionMutationR118GPromoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
JN607: pMVP/SB-DEST
Plasmid#121860PurposepMVP destination vector, empty Sleeping Beauty transposon vector backboneDepositorTypeEmpty backboneUseSleeping beauty transposon, pmvp gateway destinat…TagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KK402: pMVP/Zeo-DEST
Plasmid#121859PurposepMVP destination vector, empty expression vector backbone w/ Zeo selection cassetteDepositorTypeEmpty backboneUsePmvp gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-GABARAP G116A
Plasmid#123098PurposeExpresses 3xFLAG-GABARAP G116A in mammalian cells.DepositorInsertGamma-aminobutyric acid receptor-associated protein (GABARAP Human)
UseTags3xFLAGExpressionMammalianMutationGlycine 116 to AlaninePromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pME-myristo
Plasmid#109541PurposeMultisite gateway vector for 5' tagging with the 5' myristoylation sequence from ARF5DepositorInsert5' myristoylation sequence from ARF5
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -