Addgene

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pICH41388

Plasmid

#50253

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter (0.4 kb), 35s (Cauliflower Mosaic Virus)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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PL06008A2C06 (smedwi2)

Plasmid

#35698

Depositor

Phillip Newmark

Article

Zayas et al Proc Natl Acad Sci U S A. 2005 Dec 20;10

Insert

Smedwi2

Use

Tags

Expression

Mutation

Promoter

T7 sense, T3 antisense

Available since

April 2, 2012

Availability

Academic Institutions and Nonprofits only
pGBW-m4164344

Plasmid

#153554

Purpose

Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

accatg Kozak-START linker

Expression

Mammalian

Mutation

Gordon et al recoded

Promoter

Available since

June 16, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGL-Rbl1UTR

Plasmid

#20884

Depositor

Robert Blelloch

Article

Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

Insert

Rbl1 (p107) 3'UTR (Rbl1 Mouse)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only
OAH2

Plasmid

#64074

Purpose

Expression of O-acetyl-L-homoserine sulfhydrylase (OAH2) of Thermus thermophilus

Depositor

Vern Schramm

Article

Poulin et al J Org Chem. 2015 May 15;80(10):5344-7. d

Insert

O-acetyl-L-homoserine sulfhydrylase

Use

Tags

none

Expression

Bacterial

Mutation

Promoter

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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Sticky-147-4X

Plasmid

#158575

Purpose

Production of DNA fragment for nucleosome core particle preparation

Depositor

Curt Davey

Article

Sharma et al Nat Commun. 2019 Dec 17;10(1):5751. doi:

Type

Empty backbone

Use

Bacterial vector for dna production

Tags

Expression

Mutation

Promoter

Available since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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UbcH10 DN

Plasmid

#8505

Depositor

Joan Ruderman

Article

Townsley et al Proc Natl Acad Sci U S A 1997 Mar 18;94(

Insert

UbcH10 (UBE2C Human)

Use

Tags

Expression

Bacterial

Mutation

C114S, L118S

Promoter

Available since

June 1, 2005

Availability

Academic Institutions and Nonprofits only
pICSL80014

Plasmid

#50320

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, YFP variant of GFP (A. victoria)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

March 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGBT-PX

Plasmid

#31824

Depositor

Stephen Ekker

Article

Petzold et al Proc Natl Acad Sci U S A. 2009 Nov 3;106

Type

Empty backbone

Use

Cre/Lox; Gene trap

Tags

GFP GM2 variant

Expression

Mutation

Promoter

beta Actin

Available since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only

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piHGpd/p82

Plasmid

#1105

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

wild-type HSP82 regulated by the strong constitut…

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pICH41373

Plasmid

#50252

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter (1.3 kb), 35s (c)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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p3E-nlsGFP pA

Plasmid

#80814

Purpose

nlsGFP with pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

nlsGFPpA 3'E

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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piHGpd/G170D

Plasmid

#1113

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

HSP82 with glycine 170 changed to aspartic acid; …

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
FuPick-shRNA

Plasmid

#31614

Depositor

Robert Malenka

Article

Citri et al J Neurosci. 2010 Dec 8. 30(49):16437-52.

Insert

PICK1 shRNA (Pick1 Rat)

Use

Lentiviral and RNAi

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pICSL50014

Plasmid

#50313

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

C terminal HBP tag (HA-PreScission-Biotin)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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p3E-CMVmin:GFPmem pA

Plasmid

#80823

Purpose

CMVmin-promoted GFPmem with pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

3E-CMV-GFPCAAXpA spacer

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pVKG1-Puro

Plasmid

#108670

Purpose

Puromycin resistant cassette for knock-in by VIKING method

Depositor

Shigeo Sugano

Article

Sawatsubashi et al Sci Rep. 2018 Jan 12;8(1):593. doi: 10.1

Insert

PURO

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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3243 pSHLox Hox 11 L2

Plasmid

#8762

Depositor

Stanley Korsmeyer

Article

Shirasawa et al Nat Med 1997 Jun;3(6):646-50.

Insert

Hox11 L2 (Tlx3 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pNP154

Plasmid

#106360

Purpose

A vector backbone, including mKate, to be used to make constructs to be inserted as a single copy at a site on Chr II.

Depositor

Jonathan Ewbank

Article

Taffoni et al Elife. 2020 Jan 29;9. pii: 45047. doi: 1

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCZ45ai

Plasmid

#16469

Depositor

Bert Vogelstein

Article

Thiagalingam et al Proc Natl Acad Sci U S A. 1995 Jun 20. 9

Insert

p53 binding site (TP53 Human)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only