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Plasmid#120251PurposeCRISPR-Cas9 SunTag system to target VP64 to specific loci of interest (nog=no guide)DepositorInsertNOS_NLS_GB1_NLS_linker_VP64_linker sfGFP_scFv_UBQ10_Insulator_ UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
LRP6N-IgG-pCS2
Plasmid#27279DepositorInsertlow density lipoprotein receptor-related protein 6 (LRP6 Human)
UseXenopus injectionTagsIgG FcExpressionMutationPromoterAvailable sinceFeb. 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMK365 (Tet-OsTIR1-loxP-PURO-loxP)
Plasmid#121185PurposeOsTIR1 introductionDepositorInsertTet-OsTIR1-loxP-PURO-loxP
UseCRISPRTagsExpressionMutationOsTIR1 codon optimized for human cellsPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_cAMPFIRE-H
Plasmid#182285PurposeMammalian expression of the cAMP sensor cAMPFIRE-H under a CAG promotor w/ WPRE translation enhancer.DepositorInsertcAMPFIRE-H (cAMP sensor)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
PERK 2: PERK.K618A.9E10.pCDNA
Plasmid#21815DepositorInsertPERK (K618A) (Eif2ak3 Mouse)
UseTagsMyc (9E10)ExpressionMammalianMutationkinase dead K618A mutantPromoterAvailable sinceOct. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_cAMPFIRE-M
Plasmid#182284PurposeMammalian expression of the cAMP sensor cAMPFIRE-M under a CAG promotor w/ WPRE translation enhancer.DepositorInsertcAMPFIRE-M (cAMP sensor)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
ChromosomesMembranesandCytoskeleton
Plasmid#62450PurposeMXS_chaining vector with 4 cassettesDepositorInsertsCMV::H2B-TagBFPx3-bGHpA
CMV::Lyn-Ceruleanx3-bGHpA
CMV::mCherryx3-linker-betaActin-bGHpA
CMV::Citrinex3-linker-alphaTubulin-bGHpA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
ins:CFP-NTR
Plasmid#66593PurposeSpecific and conditional cell ablation of beta cells in the zebrafish pancreasDepositorInsertInsulin promotor driving CFP-nitroreductase
UseZebrafish expressionTagsCFP fused with N-terminus of nitroreductaseExpressionMutationPromoterZebrafish Insulin promoter (approximately 1.2kb)Available sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_cAMPFIRE-L
Plasmid#182283PurposeMammalian expression of the cAMP sensor cAMPFIRE-L under a CAG promotor w/ WPRE translation enhancer.DepositorInsertcAMPFIRE-L (cAMP sensor)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
V5-LOV-Turbo-ERM
Plasmid#199664Purposeexpresses LOV-Turbo on the mammalian ER membrane, lentiviral vectorDepositorInsertLOV-Turbo
UseLentiviralTagsSEC61B and V5ExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET29b-AK1-His
Plasmid#124135PurposeBacterial expression plasmid for PURE system componentsDepositorInsertAdenylate kinase (AK1 Chicken)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-C7orf59
Plasmid#42332DepositorInsertC7orf59 (LAMTOR4 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV, SP6Available sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
V5-LOV-Turbo-NLS
Plasmid#199663Purposeexpresses LOV-Turbo in the mammalian nucleus, lentiviral vectorDepositorInsertLOV-Turbo
UseLentiviralTagsNLS and V5ExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1_SEPT9 i1
Plasmid#71622Purposemammalian expression of human SEPT9 with an mCherry fusionDepositorInsertSeptin 9 (SEPTIN9 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
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PERK 3: PERK.dC.9E10.pCDNA
Plasmid#21816DepositorInsertPERK (C-term deleted) (Eif2ak3 Mouse)
UseTagsMyc (9E10)ExpressionMammalianMutationamino-acids 582-1081 deletedPromoterAvailable sinceJuly 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
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pMK425 (GAL-OsTIR1(F74A))
Plasmid#140657PurposeGAL-OsTIR1(F74A)DepositorInsertGAL-OsTIR1(F74A)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFS_0453_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)_T7term_ARRAY2_FaqI
Plasmid#117006PurposeExpresses E. coli codon-optimized FsRT-Cas1-Cas2 under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSV human p85 alpha (HA tag)
Plasmid#11499DepositorInsertp85 alpha (PIK3R1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only