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Plasmid#221472PurposeGateway cloning of NKX2-1 short isoform overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
U6-BbsI-GGsite-mCherry-Puro
Plasmid#221232PurposeCloning vector for sgRNA with mCherry and PuroDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-QF2-he1.1:YFP
Plasmid#184819PurposeModified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Backbone into which homology arms are cloned for CRISPR/Cas9-mediated integration of QF2.DepositorArticleInsertQF2-pA-he1.1:YFP
UseCRISPR; Backbone for cloning of homology arms (fo…TagsExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM040_P8b_5'Ura4
Plasmid#216461Purpose5' homology arm for S. pombe genomic integration. Part type 8b following the YeastToolkit MoClo grammar.DepositorInsert5'ura4
UseSynthetic BiologyTagsExpressionYeastMutationRemoval of any BsaI // BsmBI restriction sitePromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM036_P7_3"Ura4
Plasmid#216465Purpose3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.DepositorInsert3''ura4
UseSynthetic BiologyTagsExpressionYeastMutationRemoval of any BsaI // BsmBI restriction sitePromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM024_P2_pMPA1
Plasmid#216449PurposeConstitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.DepositorInsertPromoter mpa1
UseSynthetic BiologyTagsExpressionYeastMutationRemoval of any BsaI // BsmBI restriction sitePromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM022_P2_pRPS1002
Plasmid#216447PurposeConstitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.DepositorInsertPromoter rps1002
UseSynthetic BiologyTagsExpressionYeastMutationRemoval of any BsaI // BsmBI restriction sitePromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM020_P2_pTRS1
Plasmid#216445PurposeConstitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.DepositorInsertPromoter trs1
UseSynthetic BiologyTagsExpressionYeastMutationRemoval of any BsaI // BsmBI restriction sitePromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM014_P2_pGPM1
Plasmid#216439PurposeConstitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.DepositorInsertPromoter gpm1
UseSynthetic BiologyTagsExpressionYeastMutationRemoval of any BsaI // BsmBI restriction sitePromoterAvailable sinceMay 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM042_P15678_MultiGeneBackbone
Plasmid#216467PurposeEmpty integration vector for genomic integration for multigene into S. pombe. Targets Ura4 locus. Part type 15678 following the YeastToolkit MoClo grammar. Contains GFP drop out.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM041_P15678_SingleGeneBackbone
Plasmid#216466PurposeEmpty integration vector for genomic integration of 1 transcriptional unit into S. pombe. Targets Ura4 locus. Part type 15678 following the YeastToolkit MoClo grammar. Contains GFP drop out.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM033_P7_3"His5
Plasmid#216458Purpose3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.DepositorInsert3''his5
UseSynthetic BiologyTagsExpressionYeastMutationRemoval of any BsaI // BsmBI restriction sitePromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM032_P4_tSynth30
Plasmid#216457PurposeSynthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.DepositorInsertTerminator synth30
UseSynthetic BiologyTagsExpressionYeastMutationRemoval of any BsaI // BsmBI restriction sitePromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM031_P4_tSynth29
Plasmid#216456PurposeSynthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.DepositorInsertTerminator synth29
UseSynthetic BiologyTagsExpressionYeastMutationRemoval of any BsaI // BsmBI restriction sitePromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM030_P4_tSynth27
Plasmid#216455PurposeSynthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.DepositorInsertTerminator synth27
UseSynthetic BiologyTagsExpressionYeastMutationRemoval of any BsaI // BsmBI restriction sitePromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM029_P4_tSynth25
Plasmid#216454PurposeSynthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.DepositorInsertTerminator synth25
UseSynthetic BiologyTagsExpressionYeastMutationRemoval of any BsaI // BsmBI restriction sitePromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM028_P4_tSynth3
Plasmid#216453PurposeSynthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.DepositorInsertTerminator synth3
UseSynthetic BiologyTagsExpressionYeastMutationRemoval of any BsaI // BsmBI restriction sitePromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM027_P4_tSynthGuo
Plasmid#216452PurposeSynthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.DepositorInsertTerminator guo
UseSynthetic BiologyTagsExpressionYeastMutationRemoval of any BsaI // BsmBI restriction sitePromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM026_P2_pNMT1
Plasmid#216451PurposeRegulatable promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.DepositorInsertPromoter nmt1
UseSynthetic BiologyTagsExpressionYeastMutationRemoval of any BsaI // BsmBI restriction sitePromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM025_P2_pTUP11
Plasmid#216450PurposeConstitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.DepositorInsertPromoter tup11
UseSynthetic BiologyTagsExpressionYeastMutationRemoval of any BsaI // BsmBI restriction sitePromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only