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pILGFP3
Plasmid#65451PurposepILGFP3 was designed to test the promoter activity using the yeast enhanced green fluorescent protein (yEGFP) as reporter.DepositorTypeEmpty backboneUseTagsyEGFP reporterExpressionYeastMutationPromoterAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pITGFP3
Plasmid#65452PurposepITGFP3 was designed to test the promoter activity using the destabilized yeast enhanced green fluorescent protein (yEGFP-CLN2PEST) as reporter.DepositorTypeEmpty backboneUseTagsyEGFP-CLN2PEST reporterExpressionYeastMutationPromoterAvailable sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBSU6-shTim23/CMV-eGFP
Plasmid#89795PurposeExpresses shRNA against TIM23 with a separate promoter for expression of GFP.DepositorInsertshRNA Tim23 (Timm23 Mouse)
UseTagseGFP under separate promoterExpressionMammalianMutationPromoterU6Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOS019E: Entry vector for: ClopHensorN chloride sensor (cytosolic)
Plasmid#163066PurposeEntry vector for: ClopHensorN chloride sensor (cytosolic)DepositorInsertClopHensorN
UseGateway entry vectorTagstdTomatoExpressionMutationPromoterAvailable sinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMOD8A-iRGR-r11
Plasmid#74369PurposeThis plasmid constitutively expresses gRNA-r11 from an AHD1 promoter using the insulated RGR design. Integrates in the W303 HIS locus and restores HIS function.DepositorArticleInsertRGR-r11
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAHD1Available sinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
3XFlag DVL2 (E499-G)
Plasmid#24803DepositorInsertDVL2 (DVL2 Human)
UseTags3XFlagExpressionMammalianMutationE499GPromoterAvailable sinceJune 22, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYZ_3WJrep_N10_trigger
Plasmid#132728PurposeT7 RNAP-driven expression of 3WJ repressor index 10 trigger RNADepositorInsert3WJ_N10_trigger
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYZ_3WJrep_N10_switch
Plasmid#132724PurposeT7 RNAP-driven expression of 3WJ repressor index 10 switch RNA with a GFP-ASV reporterDepositorInsert3WJ_N10_switch
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
SRS-MTBD(26/19) AB(monomer), wt
Plasmid#22381DepositorInsertSeryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein
UseTagsmycExpressionBacterialMutationPromoterAvailable sinceNov. 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET11a-CA(A14C, E45C, A92E)
Plasmid#79046PurposeBacterial vector for cloning and propagation in DH5alphaDepositorInsertCA (A14C, E45C, A92E)
UseTagsExpressionBacterialMutationA14C, E45C, A92E mutations in HIV-1 CA proteinPromoterT7Available sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO.shNkx2-1
Plasmid#32400DepositorInsertshRNA targetting Nkx2-1 (Nkx2-1 Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 SV40-AcrIIA4 (GB3333)
Plasmid#160605PurposeSV40-AcrIIA4 codon optimized for N.benthamianaDepositorInsertAcrIIA4
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASp-Egl-Trbo
Plasmid#179993PurposeExpresses Egl fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system.DepositorInsertEgl-Trbo
UseTagsTurboIDExpressionInsectMutationPromoterUAS-Gal4Available sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3-AD010
Plasmid#116851PurposeExpresses araC constitutively. Expresses alpha-hemolysin and mScarletI (RFP) under a pBAD promoter; alternate name pSB1A2-AD010DepositorInsertsAraC
alpha-HL
mScarletI
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpBADAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5 DN-Src
Plasmid#19965DepositorInsertSrc (SRC Chicken)
UseTagsExpressionMammalianMutationK295MPromoterAvailable sinceDec. 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
JPF0512
Plasmid#124003PurposeEncodes promoter-less expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vectorDepositorInsertPB_spacer-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3XFLAG-MKK4-EE-NLS hygro
Plasmid#87779PurposeInducible expression of constitutively active mutant MKK4 with a C-terminal NLS sequenceDepositorInsertMKK4
UseLentiviralTags3xFLAG and NLSExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.GFP.miR-617.sensor.PGK.dNGFR.WPRE
Plasmid#85921PurposeSensor PlasmidDepositorInsertmiR-617 Sensor (MIR617 Human)
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPPC009.106
Plasmid#171142PurposeExpression of J106 scRNA on pBBR1-KmR plasmidDepositorInsertJ106 scRNA
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119Available sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only
