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Plasmid#220920PurposeNeuronal specific expression of PKM1DepositorInsertPKM1 (PKM Human)
UseLentiviralTagsExpressionMammalianMutationPromoterhSynAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
peGFP C3-SPG20 8xV
Plasmid#218579PurposeExpresses GFP-tagged human SPG20 bulky hydrophobic amino acid mutant in mammalian cellsDepositorInsertSPG20 (SPART Human)
UseTagseGFPExpressionMammalianMutationchanged Tryptophan 419, Leucine 438, Isoleucine 4…PromoterCMVAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYH78
Plasmid#217386PurposePtrc99A-PAmCherry for bacterial expressionDepositorInsertPAmCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF1674
Plasmid#142369PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRUNX1T1 (RUNX1T1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF1676
Plasmid#143832PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRUNX1T1 (RUNX1T1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-LIC_DDB1:1-1140_delta:396-689
Plasmid#210897PurposeInsect expression of full-length human DDB1 with a loop deletion I396 to D689. Untagged.DepositorInsertDDB1:M1-H1140_delta:I396-D689 (DDB1 Human)
UseTagsExpressionInsectMutationloop deletion amino acids I396 to D689PromoterPolyhedrinAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF1769
Plasmid#144192PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMGA (MGA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
mc2 blast
Plasmid#206217PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-EGFP barcode-2-SV40 polyA
Plasmid#190866PurposeFor barcoded retrograde labelingDepositorInsertEGFP-barcode2
UseAAVTagsExpressionMutationWTPromoterAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3226
Plasmid#144702PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertFGF3 (FGF3 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T_PV1_VP1
Plasmid#202071PurposeBacterial expression of VP1 protein from poliovirus 1 (PV1). Contains N-terminal GST-tag and C-terminal His6-tag.DepositorInsertPV1 VP1 protein with Histag
UseTags6xHis-tag and GSTExpressionBacterialMutationPromotertacAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2603
Plasmid#143373PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF471 (ZNF471 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1527
Plasmid#143128PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMXI1 (MXI1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1678
Plasmid#143834PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRUNX1T1 (RUNX1T1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1995
Plasmid#144376PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertKDR (KDR Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T Easy-AtpU6-26
Plasmid#160218PurposeAtpU6-26 Golden Gate level 0 piece to express gRNAsDepositorInsertpAtU6-26 promoter
UseGolden gate level 0 piece to express grnasTagsExpressionMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mDrc7-FLAG
Plasmid#176470PurposeExpression vector of mouse dynein regulatory complex subunit 7 (Drc7) tagged with FLAG at C-terminus.DepositorInsertdynein regulatory complex subunit 7 (Drc7 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCAG promoterAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mTcte1-PA
Plasmid#176469PurposeExpression vector of mouse t-complex-associated testis expressed 1 (Tcte1) tagged with PA at C-terminus.DepositorInsertt-complex-associated testis expressed 1 (Tcte1 Mouse)
UseTagsPAExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYeDP60-hATP13A2-E343A
Plasmid#171378PurposeA modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2 E343A mutant, followed by a thrombin cleavage site and a C-term BAD tag. DOI: 10.21769/BioProtoc.3888DepositorInserthuman ATP13A2 variant 2 wild-type
UseTagsBAD tagExpressionYeastMutationE343APromoterURA3Available sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-miRFP670-pA_U6-BbsI (pE059)
Plasmid#149353PurposeMammalian expression vector for miRFP670DepositorInsertmiRFP670
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only