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Plasmid#41025DepositorInserttetO7-Pcyc1-GFP
UseN-terminal tagging/promoter replacementTagsExpressionMutationPromoterAvailable sinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
1x_a1gRNAOp_pCYC1m_yeBFP2
Plasmid#64390Purposeencodes one a1 operator site upstream of minimal pCYC1 promoter driving yeast enhanced BFPDepositorInsertyeast enhanced BFP2
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJYDC2
Plasmid#162459PurposeYeast display plasmid for expression of C-terminal fusions. Modification of pJYDC1 vector with ALFA tag in MCS.DepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsALFA-tag, HA tag, HDEL sequence, and eUnaG2 fluor…ExpressionMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-hphMX-tetO-Pcyc1-3xFLAG
Plasmid#41020DepositorInserttetO7-Pcyc1-3xFLAG
UseN-terminal tagging/promoter replacementTagsExpressionMutationPromoterAvailable sinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046423
Plasmid#148998PurposeYeast Expression plasmid for SARS-CoV-2 nsp7DepositorInsertSARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
UseTagsCleavable thrombin;MBPExpressionYeastMutationwtPromoterpTEF1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046451
Plasmid#145567PurposeYeast Expression plasmid for SARS-CoV-2 nsp6DepositorInsertSARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
UseTagsCleavable thrombin;6xHISExpressionYeastMutationwtPromoterpGAL1Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFA6a-natMX-tetO-Pcyc1-3xFLAG
Plasmid#41021DepositorInserttetO7-Pcyc1-3xFLAG
UseN-terminal tagging/promoter replacementTagsExpressionMutationPromoterAvailable sinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046483
Plasmid#153868PurposeYeast expression plasmid for SARS-CoV-2 nsp3DepositorInsertSARS-CoV-2 nsp3 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;6xHISExpressionYeastMutationwtPromoterpGAL1Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pACT4-DM
Plasmid#111233Purposeyeast expression vectorDepositorInsertccdB-cassette
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW ymTurquoise2
Plasmid#118453PurposeOverexpression of yeast codon-optimized mTq2DepositorInsertymTurquoise2
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX-tetO-Pcyc1-3xFLAG
Plasmid#41022DepositorInserttetO7-Pcyc1-3xFLAG
UseN-terminal tagging/promoter replacementTagsExpressionMutationPromoterAvailable sinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBIS-GALkFLP(Trp1)
Plasmid#49459Purposeoverespression of Flp step-arrest mutant in yeast, TRP1 selectionDepositorInsertFlp(H305L) (flp Budding Yeast)
UseTagsExpressionYeastMutationH305L, ‘step-arrest’ mutant,PromoterGAL10Available sinceNov. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSP-G2
Plasmid#64737PurposeYeast expression plasmidDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046633
Plasmid#149161PurposeYeast Expression plasmid for SARS-CoV-2 ORF3a proteinDepositorInsertSARS-CoV-2 ORF3a protein (ORF3a Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
UseTagsExpressionYeastMutationwtPromoterpTEF1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046662
Plasmid#149221PurposeYeast Expression plasmid for SARS-CoV-2 ORF3a proteinDepositorInsertSARS-CoV-2 ORF3a protein (ORF3a Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
UseTagsExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpTEF1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046690
Plasmid#149160PurposeYeast Expression plasmid for SARS-CoV-2 ORF3a proteinDepositorInsertSARS-CoV-2 ORF3a protein (ORF3a Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
UseTagsExpressionYeastMutationwtPromoterpGAL1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046228
Plasmid#145400PurposeYeast Expression plasmid for SARS-CoV-2 ORF7a proteinDepositorInsertSARS-CoV-2 ORF7a protein (ORF7a Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
UseTagsCleavable thrombin;3xFLAGExpressionYeastMutationwtPromoterpTEF1Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMZ222
Plasmid#52223PurposeConstitutive expression of humanized Cas9DepositorInsertCas9
UseTagsExpressionYeastMutationsilent mutation in the original CspCI sitePromoteradh1Available sinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-hygMV
Plasmid#73891Purposefission yeast drug selection markerDepositorInserthygMV
UseTagsExpressionBacterial and YeastMutationPromoterpombe TEFAvailable sinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIYC05
Plasmid#64742PurposeYeast expression plasmid: acsL641P, ALD6DepositorInsertacsL641P, ALD6
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only