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Plasmid#126434PurposeTo target a protospacer with GA at the cut site.DepositorInsertspacer against human genome with GA at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL512 CG sgRNA
Plasmid#126432PurposeTo target a protospacer with CG at the cut site.DepositorInsertspacer against human genome with CG at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL063 GT sgRNA
Plasmid#126437PurposeTo target a protospacer with GT at the cut site.DepositorInsertspacer against human genome with GT at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL516 CT sgRNA
Plasmid#126433PurposeTo target a protospacer with CT at the cut site.DepositorInsertspacer against human genome with CT at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL515 AA sgRNA
Plasmid#126426PurposeTo target a protospacer with AA at the cut site.DepositorInsertspacer against human genome with AA at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL511 CC sgRNA
Plasmid#126431PurposeTo target a protospacer with CC at the cut site.DepositorInsertspacer against human genome with CC at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL508 TA sgRNA
Plasmid#126438PurposeTo target a protospacer with TA at the cut site.DepositorInsertspacer against human genome with TA at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL250 TC sgRNA
Plasmid#126439PurposeTo target a protospacer with TC at the cut site.DepositorInsertspacer against human genome with TC at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL514 TG sgRNA
Plasmid#126440PurposeTo target a protospacer with TG at the cut site.DepositorInsertspacer against human genome with TG at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL513 TT sgRNA
Plasmid#126441PurposeTo target a protospacer with TT at the cut site.DepositorInsertspacer against human genome with TT at cut site
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
St1Cas9_LMD-9_sgRNA_pUC19
Plasmid#110627PurposeU6-driven sgRNA expression plasmid for Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9).DepositorInsertEmpty St1Cas9 LMD-9 sgRNA backbone
UseCRISPR and Synthetic BiologyTagsNoneExpressionMammalianMutationPromoterhU6Available sinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-fsaA
Plasmid#102286PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting fsaA.DepositorInsertfsaA gRNA
UseCRISPRTagsExpressionBacterialMutationPromoterpTetAvailable sinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-adhE
Plasmid#102290PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting adhE.DepositorInsertadhE gRNA
UseCRISPRTagsExpressionBacterialMutationPromoterpTetAvailable sinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-ompT
Plasmid#102291PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting ompT.DepositorInsertompT gRNA
UseCRISPRTagsExpressionBacterialMutationPromoterpTetAvailable sinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX458-sgRNA_Ago1_4
Plasmid#73536PurposeExpresses SpCas9, GFP, and sgRNA targeting Ago1DepositorInsertAGO1
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
EMX1*_sgRNA
Plasmid#100558PurposeExpresses EMX1* sgRNA. Target sequence: (G)GAGTCCGAGCAGAAGAAGAA. Same target sequence as #100555, except with an additional 5' G.DepositorInsertEMX1 sgRNA
UseTagsExpressionMutationPromoterU6Available sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-srlA
Plasmid#89965PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting srlA.DepositorInsertsrlA gRNA
UseTagsExpressionBacterialMutationPromoterpTetAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-rbsK
Plasmid#89963PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting rbsK.DepositorInsertrbsK gRNA
UseTagsExpressionBacterialMutationPromoterpTetAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-yhhT
Plasmid#89958PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting yhhT.DepositorInsertyhhT gRNA
UseTagsExpressionBacterialMutationPromoterpTetAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-rssA
Plasmid#89957PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting rssA.DepositorInsertrssA gRNA
UseTagsExpressionBacterialMutationPromoterpTetAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only