-
Plasmid#181947PurposeaTc-inducible expression of TorR with N-terminal mNeonGreen fusion. Also contains mCherry under TorR-controlled promoter PtorCAD129DepositorInsertUseTagsmNeonGreenExpressionBacterialMutationPromotermNG-TorR-PLtetO-1; mCherry-PtorCAD129; tetR-J23106Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only
-
pRB133.2
Plasmid#181950PurposeaTc-inducible expression of PhoP with C-terminal mNeonGreen fusion. Also contains mCherry under PhoP-controlled promoter PvirKDepositorInsertPhoP-Salmonella enterica subsp. enterica serovar Typhimurium (AX04_RS23485 Synthetic, PhoP-Salmonella enterica subsp. enterica serovar Typhimurium)
UseTagsmNeonGreenExpressionBacterialMutationPromoterPhoP-mNG-PLtetO-1; mCherry-PvirK; tetR-J23106Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSIIpuro-mNG-eDHFR(69K6)
Plasmid#178853PurposeMammalian expression of mNeonGreen-eDHFR(69K6)DepositorInsertmNeonGreen-eDHFR(69K6)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1-alphaAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pN1-COX8-mNG
Plasmid#129342PurposeMitochondria matrix localizationDepositorInsertmNeonGreen
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pN1-TOMM20-mNG
Plasmid#129347PurposeMitochondrial outer membrane localizationDepositorInsertmNeonGreen
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE3g-mNeon-WPRE-pA
Plasmid#192064PurposemNeonGreen expression with TRE3g promoterDepositorInsertmNeonGreen
UseAAVTagsExpressionMammalianMutationPromoterTRE3gAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Amp_CRISPIE donor B4
Plasmid#172845PurposeCRISPIE donor B4 (Zhong et al, eLife 2021), mNeonGreen translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).DepositorInsertCRISPIE designer exon (phase 0-0) encoding mNeonGreen
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-mNG-eDHFR(69K6)-p85iSH2
Plasmid#172103PurposeMammalian expression of p85iSH2 fused to mNeonGreen-eDHFR(69K6)DepositorInsertmNeonGreen-eDHFR(69K6)-p85iSH2 (Pik3r2 Mouse)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
Rtn2b-RHD272-472-GFP
Plasmid#186606PurposeEncodes human Reticulon 2 isoform B reticulon homology domain aa 272 to 472 fused to GFPDepositorInsertHuman Reticulon 2 isoform B amino acids 272 to 472 (RTN2 Human)
UseTagsmNeonGreenExpressionMammalianMutationMet-Gly-Ser-Lys peptide added at N-terminus for c…PromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pN1-OPA1(123Nt)-mNG
Plasmid#129344PurposeMitochondrial intermembrane space localizationDepositorInsertmNeonGreen
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-mNG-eDHFR(69K6)-Tiam1
Plasmid#172102PurposeMammalian expression of Tiam1(DH-PH) fused to mNeonGreen-eDHFR(69K6)DepositorInsertmNeonGreen-eDHFR(69K6)-Tiam1(DH-PH) (TIAM1 Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPB-cjOCT4-mNG-WPRE
Plasmid#214123PurposeGene expression by Piggybac transposase in mammalian cellsDepositorInsertmNeonGreen
UseTagsExpressionMammalianMutationCodon-optimized for mammalian cellsPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CBh-IgKL-mNG
Plasmid#183467PurposeTo produce AAV with the CBh promoter to express IgkL-mNeonGreen (IgKL is a secretory signal peptide)DepositorInsertIgKL-mNeonGreen
UseAAVTagsIgKL secretory signalExpressionMutationPromoterAvailable sinceMay 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJW1582
Plasmid#154312PurposemNeonGreen^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.DepositorInsertmNeonGreen^SEC^AID*::3xFlag
UseCRISPR and Cre/LoxTagsExpressionWormMutationPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-mNG-eDHFR(69K6)-G-alpha-q
Plasmid#172106PurposeMammalian expression of Gαq fused to mNeonGreen-eDHFR(69K6)DepositorInsertmNeonGreen-eDHFR(69K6)-Gαq (GNAQ Human)
UseTagsExpressionMammalianMutationGαq: C9S/C10S/Q209LPromoterCAGAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-P3-IgKL-mNG
Plasmid#183465PurposeTo produce AAV with the P3 promoter to express IgkL-mNeonGreen (IgKL is a secretory signal peptide)DepositorInsertIgKL-mNeonGreen
UseAAVTagsIgKL secretory signalExpressionMutationPromoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSIIpuro-mNG-eDHFR(69K6)-RasGEF
Plasmid#172104PurposeMammalian expression of RasGRF1(Cdc25) fused to mNeonGreen-eDHFR(69K6)DepositorInsertmNeonGreen-eDHFR(69K6)-RasGRF1(Cdc25) (RASGRF1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1-alphaAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-NRG1-ScNeo
Plasmid#209911PurposeTo monitor the status of NRG1, the plasmid encodes a recombinant NRG1 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorInsertNRG1-ScNeo (NRG1 Human)
UseTagsmNeonGreen and mScarletExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCSIIpuro-BTC-ScNeo
Plasmid#209898PurposeTo monitor the status of Betacellulin, the plasmid encodes a recombinant BTC fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorInsertBTC-ScNeo (BTC Human)
UseLentiviralTagsmNeonGreen and mScarletExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCSIIpuro-NRG1-ScNeo
Plasmid#209900PurposeTo monitor the status of NRG1, the plasmid encodes a recombinant NRG1 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorInsertNRG1-ScNeo (NRG1 Human)
UseLentiviralTagsmNeonGreen and mScarletExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only