Addgene

We narrowed to 1,578 results for: levi

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pET28a-P5CR

Plasmid

#110295

Purpose

Expresses pyrroline-5-carboxylate reductase in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Science. 2017 Feb 10;355(6325). pii: 355

Insert

Pyrroline-5-carboxylate Reductase

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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pRS416-PHO5-GFP-2xOsh2 PH domain EE mutation

Plasmid

#58830

Purpose

Expresses GFP-Tagged and mutated Osh2 PH domain in yeast

Depositor

Tim Levine

Article

Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

Insert

2 x Osh2 PH domain (OSH2 Budding Yeast)

Use

Tags

GFP and Myc

Expression

Yeast

Mutation

changed Lysine 307 to Glutamate and Arginine 309 …

Promoter

PHO5

Available since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
D10 pAcGP67a

Plasmid

#41812

Depositor

Chris Garcia

Article

Levin et al Nature. 2012 Mar 25;484(7395):529-33. do

Insert

D10 Super-2 (IL2 Human)

Use

Tags

8x His

Expression

Insect

Mutation

Promoter

Polyhedron

Available since

Jan. 8, 2013

Availability

Academic Institutions and Nonprofits only
pET28a-HypD

Plasmid

#110288

Purpose

Expresses t-4-hydroxy-L-proline dehydratase in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Science. 2017 Feb 10;355(6325). pii: 355

Insert

t-4-Hydroxy-L-proline Dehydratase

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-PDAE

Plasmid

#110180

Purpose

Expresses propanediol dehydratase activating enzyme in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Science. 2017 Feb 10;355(6325). pii: 355

Insert

Propanediol Dehydratase Activating Enzyme

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pET29b-PD

Plasmid

#110168

Purpose

Expresses propanediol dehydratase in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Science. 2017 Feb 10;355(6325). pii: 355

Insert

Propanediol Dehydratase

Use

Tags

His6 and S-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCVD419

Plasmid

#63754

Purpose

3.4 kb HindIII fragment from EHEC associated plasmid from E. coli 0157:H7 strain 933 cloned into pBR325. Contains the hlyA gene. Best EHEC probe for detection of this plasmid.

Depositor

James Kaper

Article

Levine et al J Infect Dis. 1987 Jul;156(1):175-82.

Insert

ehxA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 6, 2015

Availability

Academic Institutions and Nonprofits only
pENTR1A FKBP12F36V-OGT

Plasmid

#154287

Purpose

Entry vector encoding FKBP12(F36V)-2xHA-OGT (dTAG fused-O-GlcNAc Transferase)

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Gateway entry vector

Tags

2x HA and FKBP12(F36V)

Expression

Mutation

Promoter

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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IL2 pMalLP

Plasmid

#41808

Depositor

Chris Garcia

Article

Levin et al Nature. 2012 Mar 25;484(7395):529-33. do

Insert

IL2 (IL2 Human)

Use

Tags

3C protease site, 6x His, and Maltose Binding Pro…

Expression

Bacterial

Mutation

Promoter

pTac

Available since

Jan. 4, 2013

Availability

Academic Institutions and Nonprofits only

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NEK7_HUMAN_D0

Plasmid

#79735

Purpose

This plasmid encodes the kinase domain of NEK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

NEK7 (NEK7 Human)

Use

Tags

His10-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pLEX_305-N-dTAG_OGT

Plasmid

#155196

Purpose

N-terminal FKBP12(F36V)-2xHA tag on lentiviral OGT construct

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Lentiviral

Tags

2x HA and FKBP12(F36V)

Expression

Mammalian

Mutation

lacking N-terminal Met

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR1A mKate2-hu_ncOGT WT

Plasmid

#154282

Purpose

Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; WT)

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Gateway entry vector

Tags

2x FLAG and mKate2

Expression

Mutation

WT OGT

Promoter

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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KCC2B_HUMAN_D0

Plasmid

#79718

Purpose

This plasmid encodes the kinase domain of KCC2B. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KCC2B

Use

Tags

His10-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
MK14_HUMAN_D0

Plasmid

#79680

Purpose

This plasmid encodes the kinase domain of MK14. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MK14

Use

Tags

His10-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pENTR1A hu_ncOGT WT (open N-term)

Plasmid

#155197

Purpose

Entry vector encoding non-tagged O-GlcNAc Transferase with open N-terminus

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Gateway cloning

Tags

Expression

Mutation

WT OGT with no start codon, in-frame with gateway…

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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H9 pMalLP

Plasmid

#41811

Depositor

Chris Garcia

Article

Levin et al Nature. 2012 Mar 25;484(7395):529-33. do

Insert

H9 Super-2 (IL2 Human)

Use

Tags

3C protease site, 6x His, and Maltose Binding Pro…

Expression

Bacterial

Mutation

Promoter

pTac

Available since

Jan. 8, 2013

Availability

Academic Institutions and Nonprofits only
pLenti PGK OGT (Neo)

Plasmid

#154289

Purpose

Lentiviral vector encoding full length wildtype human OGT

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
VGFR1_HUMAN_D0

Plasmid

#79743

Purpose

This plasmid encodes the kinase domain of VGFR1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

VGFR1

Use

Tags

His10-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
IL2 F42A pAcGP67a

Plasmid

#41807

Depositor

Chris Garcia

Article

Levin et al Nature. 2012 Mar 25;484(7395):529-33. do

Insert

IL2 (IL2 Human)

Use

Tags

6x His

Expression

Insect

Mutation

Phenylalanine 42 to Alanine

Promoter

Polyhedron

Available since

Jan. 3, 2013

Availability

Academic Institutions and Nonprofits only
PAK7_HUMAN_D0

Plasmid

#79706

Purpose

This plasmid encodes the kinase domain of PAK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

PAK7 (PAK5 Human)

Use

Tags

His10-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only