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Plasmid#50912PurposeExpresses human NKCC1 with truncation of N-terminus, mutations at P676C I730C and C-terminus replaced with that of NBCe. Contains an N-terminal 3xFLAG-YFP tag for expression in mammalian cellsDepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and YFPExpressionMammalianMutationP676C and I730C; N-term deletion of aa13-222; C-t…PromoterCMVAvailable sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
WNV NS2B-NS3 protease (based on PDB 2GGC, H106A - catalytically inactive); aliases: West Nile Virus NS2B-NS3 fusion protein
Plasmid#204797PurposeBacterial expression of a fusion of West Nile NS2B-NS3 proteinsDepositorInsertWest Nile NS2B-NS3 fusion
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDEST14-SpyCatcher002
Plasmid#102827PurposeBacterial expression of SpyCatcher002 for covalent coupling to SpyTag002-linked proteinsDepositorInsertSpyCatcher002
UseTagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher003
Plasmid#133447PurposeExpresses SpyCatcher003 in bacterial cytoplasmDepositorInsertSpyCatcher003
UseTagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyTag002-MBP
Plasmid#102831PurposeBacterial expression of SpyTag002 fused to maltose binding proteinDepositorInsertSpyTag002-MBP
UseTagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to the N-terminus of MBP via a …PromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher003-sfGFP
Plasmid#133449PurposeExpresses SpyCatcher003-superfolder GFP in bacterial cytoplasmDepositorInsertSpyCatcher003-sfGFP
UseTagsHis6 and TEV siteExpressionBacterialMutationPromoterT5Available sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-sfGFP
Plasmid#133454PurposeExpresses SpyTag003-superfolder GFP in bacterial cytoplasmDepositorInsertSpyTag003-sfGFP
UseTagsGSGSGS linker and His6ExpressionBacterialMutationPromoterT7Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-mKate2
Plasmid#133452PurposeExpresses SpyTag003-mKate2 (a far-red fluorescent protein) in bacterial cytoplasmDepositorInsertSpyTag003-mKate2
UseTagsGSGSGS linker and His6ExpressionBacterialMutationPromoterT7Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpyTag-sfGFP
Plasmid#184226PurposeExpresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.DepositorInsertSpyTag-sfGFP
UseTagsHis6, SpyTag, and TEV protease cleavage siteExpressionBacterialMutationPromoterT5Available sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oDi
Plasmid#124661PurposeExpresses SpyCatcher002-oDi for dimerization of SpyTag-proteins via dimeric coiled-coilDepositorInsertSpyCatcher002-oDi
UseTagsC-tag, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oTet
Plasmid#124663PurposeExpresses SpyCatcher002-oTet for tetramerization of SpyTag-proteins via tetrameric coiled-coilDepositorInsertSpyCatcher002-oTet
UseTagsC-tag, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oTri
Plasmid#124662PurposeExpresses SpyCatcher002-oTri for trimerization of SpyTag-proteins via trimeric coiled-coilDepositorInsertSpyCatcher002-oTri
UseTagsC-tag, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oHex
Plasmid#124670PurposeExpresses SpyCatcher002-oHex for hexamerization of SpyTag-proteins via hexameric coiled-coilDepositorInsertSpyCatcher002-oHex
UseTagsC-tag, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oHept
Plasmid#124671PurposeExpresses SpyCatcher002-oHept for heptamerization of SpyTag-proteins via heptameric coiled-coilDepositorInsertSpyCatcher002-oHept
UseTagsC-tag, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-mClover3
Plasmid#133453PurposeExpresses SpyTag003-mClover3 (a green fluorescent protein) in bacterial cytoplasmDepositorInsertSpyTag003-mClover3
UseTagsGSGSGSGS linker and His6ExpressionBacterialMutationPromoterT7Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-Avitag-SpyCatcher002
Plasmid#102828PurposeBacterial expression of SpyCatcher002 with a peptide tag enabling site-specific biotinylationDepositorInsertAvitag-SpyCatcher002
UseTagsAvitag, GSSGS, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-Cys-SpyCatcher002
Plasmid#102829PurposeBacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagentsDepositorInsertCys-SpyCatcher002
UseTagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationCysteine residue in N-terminal regionPromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oPent
Plasmid#124664PurposeExpresses SpyCatcher002-oPent for pentamerization of SpyTag-proteins via pentameric coiled-coilDepositorInsertSpyCatcher002-oPent
UseTagsC-tag, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SpyCatcher002 EQ
Plasmid#102830PurposeBacterial expression of unreactive version of SpyCatcher002DepositorInsertSpyCatcher002 EQ
UseTagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationE77Q mutation prevents isopeptide bond formationPromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-MBP
Plasmid#133450PurposeExpresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasmDepositorInsertSpyTag003-MBP
UseTagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to N-terminus of MBP via a GSGE…PromoterT7Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only