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pAAV-hSyn-fDIO-mScarlet-Gphn_C4c

Plasmid

#194974

Purpose

AAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform C4c) under the control of human Synapsin promoter

Depositor

Günter Schwarz

Article

Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

Insert

mScarlet-Gphn (isoform C4c) (Gphn Synthetic, Rat)

Use

AAV

Tags

mScarlet

Expression

Mutation

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pH6-TEV-spTorA-GFP(C)

Plasmid

#169041

Purpose

GFP preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag and TEV cleavage site in pBAD24.

Depositor

Siegfried Musser

Article

Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

Insert

H6-spTorA-GFP

Use

Tags

6xHis, TEV cleavage site, and spTorA (signal pept…

Expression

Bacterial

Mutation

The mut3 GFP variant with 5 additional mutations …

Promoter

araBAD

Available since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTagGFP2-N SIN1 Na

Plasmid

#124928

Purpose

For expression of a.a. 1-68 of SIN1-GFP

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Use

Tags

TagGFP2

Expression

Mammalian

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiCMV-Blast mLST8-Flag Mut1

Plasmid

#124916

Purpose

For stable expression sgRNA resistant Flag tagging mLST8 Q44E/N46L mutant

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MLST8 (MLST8 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Insert ORF was sirently mutated to be resistent t…

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiCMV-Blast mLST8-Flag Mut2

Plasmid

#124917

Purpose

For stable expression_ sgRNA resistant Flag tagging mLST8 W272F/W274F mutant

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MLST8 (MLST8 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Insert ORF was sirently mutated to be resistent t…

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-Sec61b-C1

Plasmid

#90994

Purpose

Expresses mCherry-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

Depositor

Jennifer Lippincott-Schwartz

Article

Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

Insert

mCherry-Sec61b (SEC61B Synthetic, Human)

Use

Tags

mCherry (red fluorescence)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pTagGFP2-N SIN1

Plasmid

#124919

Purpose

For expression of wild-type SIN1-GFP

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Use

Tags

TagGFP2

Expression

Mammalian

Mutation

Insert ORF was sirently mutated to be resistent t…

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pTagGFP2-N SIN1 deltaNCD

Plasmid

#124920

Purpose

For expression of N-terminal Conserved Domain deletion mutant of SIN1-GFP (delta2-137)

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Use

Tags

TagGFP2

Expression

Mammalian

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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mEmerald-Sec61b-C1

Plasmid

#90992

Purpose

Expresses mEmerald-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

Depositor

Jennifer Lippincott-Schwartz

Article

Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

Insert

mEmerald-Sec61b (SEC61B Synthetic, Human)

Use

Tags

mEmerald (green fluorescence)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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mEmerald-GPAT4152-208

Plasmid

#134468

Purpose

GPAT4152-208 expression

Depositor

Jennifer Lippincott-Schwartz

Article

Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

Insert

GPAT4 (GPAT4 Human)

Use

Tags

Expression

Mammalian

Mutation

GPAT4, amino acids 152-208

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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mApple-Sec61b-C1

Plasmid

#90993

Purpose

Expresses mApple-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

Depositor

Jennifer Lippincott-Schwartz

Article

Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

Insert

mApple-Sec61b (SEC61B Synthetic, Human)

Use

Tags

mApple (red fluorescence)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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Halo-p58

Plasmid

#166896

Purpose

mammalian expression of p58 (ie ERGIC-53; LMAN1) tagged with HaloTag

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

p58 (Lman1 Rat)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

L405Q, R419S- please see depositor comments

Promoter

CMV

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pTagGFP2-N SIN1 delta2-29

Plasmid

#124923

Purpose

For expression of a.a.2-29 deletion mutant of SIN1-GFP

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Use

Tags

TagGFP2

Expression

Mammalian

Mutation

Insert ORF was sirently mutated to be resistent t…

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDONOR-SNCAe3-WT

Plasmid

#85847

Purpose

Donor plasmid for SNCA exon3 wild type sequence. lso contains TagBFP and dTomato

Depositor

Jens Schwamborn

Article

Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

Insert

SNCA exon 3 homology arms (SNCA Human)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pKM-U6-reRNA-hsHEKsite_1_3

Plasmid

#205638

Purpose

reRNA guide targeting HEKsite_1_3

Depositor

Gerald Schwank

Article

Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

Insert

reRNA-HEKsite_1_3 (EPHA3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pDONOR-SNCAe2-WT

Plasmid

#85845

Purpose

Donor plasmid for SNCA exon2 wild type sequence. Also contains TagBFP and EGFP

Depositor

Jens Schwamborn

Article

Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

Insert

SNCA exon 2 homology arms (SNCA Human)

Use

Tags

-

Expression

Bacterial and Mammalian

Mutation

-

Promoter

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiCMV-Blast mLST8-Flag Mut1-2

Plasmid

#124918

Purpose

For stable expression sgRNA resistant Flag tagging mLST8 Q44E/N46L and W272F/W274F mutant

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MLST8 (MLST8 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Insert ORF was sirently mutated to be resistent t…

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTagGFP2-N SIN1 delta69-97

Plasmid

#124924

Purpose

For expression of a.a.69-97 deletion mutant of SIN1-GFP

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Use

Tags

TagGFP2

Expression

Mammalian

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pTagGFP2-N SIN1 NCD

Plasmid

#124927

Purpose

For expression of NCD (a.a. 1-139) of SIN1-GFP

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Use

Tags

TagGFP2

Expression

Mammalian

Mutation

Insert ORF was sirently mutated to be resistent t…

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONOR-SNCAe3-A53T

Plasmid

#85848

Purpose

Donor plasmid for SNCA exon3 A53T sequence. Also contains EGFP and tagBFP

Depositor

Jens Schwamborn

Article

Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

Insert

SNCA exon 3 homology arms (SNCA Human)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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