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Plasmid#169244PurposeSuicide vector for integrating expression cassette in P. putida (L-rhamnose induction)DepositorInsertrhaRS
UseTagsExpressionBacterialMutationPromoterrhaSAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pChlr-4SYE
Plasmid#52156PurposeEncodes module 4 with amino acids 12S-13Y-16E for recognition of nt 5GDepositorInsertD43951 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12N to 12S, 13H to 13Y, and 16Q to 16E in…PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-3CQ
Plasmid#52147PurposeEncodes module 3 with amino acids 12C-16Q for recognition of nt 6ADepositorInsertPumilio homology domain amino acids 97-132 (PUM1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-1SQ
Plasmid#52139PurposeEncodes module 1 with amino acids 12S-16Q for recognition of nt 8ADepositorInsertPumilio homology domain amino acids 25-60 (PUM1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-2NQ
Plasmid#52145PurposeEncodes module 2 with amino acids 12N-16Q for recognition of nt 7UDepositorInsertPumilio homology domain amino acids 61-96 (PUM1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-4NQ
Plasmid#52153PurposeEncodes module 4 with amino acids 12N-16Q for recognition of nt 5UDepositorInsertPumilio homology domain amino acids 133-168 (PUM1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-5CQ
Plasmid#52159PurposeEncodes module 5 with amino acids 12C-16Q for recognition of nt 4ADepositorInsertPumilio homology domain amino acids 169-204 (PUM1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-6NQ
Plasmid#52165PurposeEncodes module 6 with amino acids 12N-16Q for recognition of nt 3UDepositorInsertPumilio homology domain amino acids 205-240 (PUM1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-8NQ
Plasmid#52173PurposeEncodes module 8 with amino acids 12N-16Q for recognition of nt 1UDepositorInsertPumilio homology domain amino acids 284-319 (PUM1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCas61
Plasmid#82397PurposeIPTG inducible, cLac143 (Markley 2015), LDH V39R with Gmr in glpKDepositorInsertLDH
UseTagsExpressionMutationV39RPromotercLac143Available sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBTRCk-pVhb-maACR
Plasmid#215651PurposepBBR1 Origin, KanR, with maACR gene under Vhb promoterDepositorInsertacyl-CoA reductase
UseTagsMaltose binding protein and His tagExpressionBacterialMutationPromoterVhbAvailable sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
ptrc99a-pVhb-tdTER-fdh
Plasmid#215649PurposepBR322 Origin, AmpR, with tdTER and Fdh genes under Vhb promoterDepositorInsertstrans-enoyl-CoA reductase
formate dehydrogenase
UseTagsExpressionBacterialMutationPromoterVhbAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYC-pVhb-vfFadBA
Plasmid#215650PurposepACYC Origin, CmR, with vfFadBA gene under Vhb promoterDepositorInsertthiolase (FadA), β-hydroxy-acyl-CoA reductase (FadB)
UseTagsExpressionBacterialMutationPromoterVhbAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pY2-MET-IV
Plasmid#218117PurposeMET-HYGRO plasmid (218133) with the functional cassettes of pY2 (218115) inserted at the sfGFP dropout locus.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pY2-LYS-IV
Plasmid#218118PurposeLYS-Leu2 plasmid (218114) with the functional cassettes of pY2 (218115) inserted at the sfGFP dropout locus.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRA1EGFP
Plasmid#193791PurposeExpresses EGFP under the control of PR promoter, pUC origin of replication, Ampicillin selectionDepositorInsertEGFP
UseTagsExpressionBacterialMutationPromoterPR promoterAvailable sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTS2369-Tier1-PhCMV_KRAB
Plasmid#169571PurposeTier-1 vector encoding PhCMV-driven KRAB transsilencer domain (PhCMV-KRAB-pA).DepositorInsertPCMV-driven Kruppel associated box domain
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH235-Tier1-PmPGK1-rtTA
Plasmid#169578PurposeTier-1 vector encoding PmPGK1-driven rtTA expression (PmPGK1-rtTA-pA).DepositorInsertreverse tetracycline-controlled transactivator
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1108_Tier3(Lenti_inverse)
Plasmid#169663PurposeTier-3 vector for stable integration of up to three cassettes via lentiviral transduction in inverted direction with polyA (PCMV-5'LTR-Psi-gag-env-A3-pA::A2-pA::A1-pA-WPRE-3'LTR)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH230-2-Tier1-PmPGK1-YPet
Plasmid#169521PurposeTier-1 vector encoding PmPGK1-driven YPet expression (PmPGK1-YPet-pA).DepositorInsertPPGK-driven yellow fluorescent protein Ypet
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only