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GFP-LaM(N)-cpFRB2-T2A-FKBP-LaM(C)

Plasmid

#165028

Purpose

Express cpFRB2-based mCherry specific Chessbody (chemically switchable split nanobody); visualized by GFP

Depositor

Yubin Zhou

Article

Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

Insert

LaM8(N)-cpFRB2-T2A-FKBP-LaM8(C)

Use

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

Promoter

T7

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pMV47

Plasmid

#128866

Purpose

Position 1 module- IX301997 (yeast integrative region IX301997)

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

Yeast integrative region chrIX301997

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pMV46

Plasmid

#128865

Purpose

Position 1 module- VI260998 (yeast integrative region VI260998)

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

Yeast integrative region chrVI260998

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0114

Plasmid

#117553

Purpose

Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Promoter_AtU6-26 + sgRNA_NbPDS1 N. benthamiana

Use

Tags

Expression

Plant

Mutation

Promoter

At U6-26 (pICSL90002)

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0029

Plasmid

#117541

Purpose

Level1 Golden Gate Cassette: sgRNA cassette for LbCas12a, targeting NbPDS gene in N. benthamiana

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

crRNA (LbCas12a)

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0028

Plasmid

#117540

Purpose

Level1 Golden Gate Cassette: sgRNA cassette for AsCas12a, targeting NbPDS gene in N. benthamiana

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

crRNA (AsCas12a)

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0014

Plasmid

#117524

Purpose

Level0 Golden Gate part: CDS, SpCas9-KA

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-KA (with stop codon)

Use

CRISPR

Tags

Expression

Mutation

K855A

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0009

Plasmid

#117522

Purpose

Level0 Golden Gate part: CDS, SpCas9-p (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-p (no stop codon)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pICSL90005

Plasmid

#117520

Purpose

Level0 Golden Gate part: CDS, SpCas9-h (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-h (no stop codon)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0008

Plasmid

#117535

Purpose

Level0 Golden Gate part: CDS, AsCas12a

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

AsCas12a (with stop codon)

Use

CRISPR

Tags

3xHA and NLS

Expression

Mutation

Promoter

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB2336

Plasmid

#67541

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XII-5 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pILGFP4G

Plasmid

#104502

Purpose

Used to evaluate the expression output of Saccharomyces cerevisiae PGM2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

ScPGM2 promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pILGFP4K

Plasmid

#104506

Purpose

Used to evaluate the expression output of Saccharomyces paradoxus GAL1 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SpGAL1 promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pILGFP4O

Plasmid

#104510

Purpose

Used to evaluate the expression output of Saccharomyces paradoxus GAL2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SpdGAL2 promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pILGFP4P

Plasmid

#104511

Purpose

Used to evaluate the expression output of Saccharomyces pastorianus GAL2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SptGAL2 promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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Expression_vector RO1_1

Plasmid

#158010

Purpose

Expression vector for the de novo designed protein Expression_vector_RO1_1

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein RO1_1

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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Expression_vector_RO1_2

Plasmid

#158011

Purpose

Expression vector for the de novo designed protein Expression_vector_RO1_2

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein RO1_2

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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Expression_vector_RO1_5

Plasmid

#158012

Purpose

Expression vector for the de novo designed protein Expression_vector_RO1_5

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein RO1_5

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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Expression_vector_RO1_8

Plasmid

#158013

Purpose

Expression vector for the de novo designed protein Expression_vector_RO1_8

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein RO1_8

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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Expression_vector_RO1_9

Plasmid

#158014

Purpose

Expression vector for the de novo designed protein Expression_vector_RO1_9

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein RO1_9

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge