Addgene

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pKT0174

Plasmid

#8720

Depositor

Kurt Thorn

Article

Sheff et al Yeast 2004 Jun;21(8):661-70.

Insert

yECFP

Use

Tags

Codon optimized linker

Expression

Yeast

Mutation

yEGFP with F64L, S65T, Y66W, N144I, M153T, V163A

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pKT0101

Plasmid

#8721

Depositor

Kurt Thorn

Article

Sheff et al Yeast 2004 Jun;21(8):661-70.

Insert

yECFP

Use

Tags

Codon optimized linker

Expression

Yeast

Mutation

yEGFP with F64L, S65T, Y66W, N146I, M153T, V163A

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pKT0232

Plasmid

#8727

Depositor

Kurt Thorn

Article

Sheff et al Yeast 2004 Jun;21(8):661-70.

Insert

yECFP

Use

Tags

13x Myc and Codon optimized linker

Expression

Yeast

Mutation

yEGFP with F64L, S65T, Y66W, N146I, M153T, V163A

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pKT0221

Plasmid

#8726

Depositor

Kurt Thorn

Article

Sheff et al Yeast 2004 Jun;21(8):661-70.

Insert

yECFP

Use

Tags

3HA and Codon optimized linker

Expression

Yeast

Mutation

yEGFP with F64L, S65T, Y66W, N146I, M153T, V163A

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pCY 3020-02

Plasmid

#36213

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

yECFP

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-yECFP-hphMX4

Expression

Mutation

Promoter

Available since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pTet-Bxb1-CFP

Plasmid

#195374

Purpose

Expresses Bxb1 integrase fused with CFP for fluorescence

Depositor

Richard Murray

Article

Pandey et al ACS Synth Biol. 2023 Feb 17;12(2):511-52

Insert

Bxb1 Integrase Fused With deCFP

Use

Synthetic Biology

Tags

deCFP

Expression

Mutation

Promoter

pTet

Available since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-InPAkt

Plasmid

#181914

Purpose

Indicator of Phosphoinositides using Akt; genetically encoded FRET-based sensor for monitoring 3-phosphoinositide dynamics in living cells.

Depositor

Jin Zhang

Article

Ananthanarayanan et al Proc Natl Acad Sci U S A. 2005 Oct 18;10

Insert

InPAkt

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pRSM13

Plasmid

#101644

Purpose

Encodes the fluorescent protein meleCFP for constitutive expression in E. coli.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

meleCFP

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PM-InPAkt

Plasmid

#181915

Purpose

Indicator of Phosphoinositides using Akt; targeted to the plasma membrane.

Depositor

Jin Zhang

Article

Ananthanarayanan et al Proc Natl Acad Sci U S A. 2005 Oct 18;10

Insert

pm-InPAkt

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Contains the following mutations with respect to …

Promoter

CMV

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
mCerulean N1

Plasmid

#27795

Depositor

Steven Vogel

Article

Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

Insert

mCerulean N1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 19, 2011

Availability

Academic Institutions and Nonprofits only

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mCerulean C1

Plasmid

#27796

Depositor

Steven Vogel

Article

Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

Insert

mCerulean C1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2011

Availability

Academic Institutions and Nonprofits only

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pKT0210

Plasmid

#8724

Depositor

Kurt Thorn

Article

Sheff et al Yeast 2004 Jun;21(8):661-70.

Insert

yECFP A206R

Use

Tags

Codon optimized linker

Expression

Yeast

Mutation

yEGFP with F64L, S65T, Y66W, N146I, M153T, V163A,…

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pKT0212

Plasmid

#8725

Depositor

Kurt Thorn

Article

Sheff et al Yeast 2004 Jun;21(8):661-70.

Insert

yECFP A206R

Use

Tags

Codon optimized linker

Expression

Yeast

Mutation

yEGFP with F64L, S65T, Y66W, N146I, M153T, V163A,…

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pDR-GW AT1.03

Plasmid

#28003

Depositor

Wolf Frommer

Article

Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Insert

AT1.03

Use

Tags

mVenus and mseCFP

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only

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peZinCh-NB

Plasmid

#24173

Depositor

Maarten Merkx

Article

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Insert

eZinCh-NB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 14, 2010

Availability

Academic Institutions and Nonprofits only

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pDR-GW AT1.03YEMK

Plasmid

#28004

Depositor

Wolf Frommer

Article

Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Insert

AT1.03YEMK

Use

Tags

mVenus and mseCFP

Expression

Yeast

Mutation

M60Y, G131E, K132M, 133K

Promoter

Available since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only

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pmCherry-histone H3

Plasmid

#179699

Purpose

visualization of nucleus/chromosome

Depositor

Kenji Sugimoto

Article

Yasui et al Mutat Res. 2010 Oct 13;692(1-2):12-8. do

Insert

Histone H3 (Hist1h3h Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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CFP-PBD

Plasmid

#11394

Depositor

Joel Swanson

Article

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Insert

p21-activated kinase 1 (PAK1 Human)

Use

Tags

CFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only
pDR-GW AT1.03R122K/R126K

Plasmid

#28005

Depositor

Wolf Frommer

Article

Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Insert

AT1.03R122K/R126K

Use

Tags

mVenus and mseCFP

Expression

Yeast

Mutation

R122K, R126K

Promoter

Available since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only
isce rfp/cfp

Plasmid

#83323

Purpose

fluorescent Isce joint substrate

Depositor

Katheryn Meek

Article

Neal et al J Immunol. 2016 Apr 1;196(7):3032-42. do

Insert

RFP +CFP joining substrate

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge