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pMK290 (mAID-mClover-Hygro)

Plasmid

#72828

Purpose

C-terminal tagging with mAID-mClover using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-mClover-Hygro

Use

CRISPR

Tags

mAID-mClover

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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AgDD

Plasmid

#78289

Purpose

create protein aggregates in living cells

Depositor

Thomas Wandless

Article

Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

Insert

NES-L106P-sfGFP (FKBP1A Human)

Use

Tags

MLALKLAGLDI

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pMax_mOrange_A215C

Plasmid

#177828

Purpose

Site-specific mutagenesis of mOrange for the purposes of creating a site-specific 8-oxo-G:C lesion for the purposes of measuring OGG1 activity via Host Cell Reactivation (HCR).

Depositor

Leona Samson, Zachary Nagel

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

mOrange_A215C

Use

Tags

Expression

Mammalian

Mutation

A215C

Promoter

CMV

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 (-) + SLNCR1 + 12x MS2 bs

Plasmid

#86828

Purpose

Expression of SLNCR1 lncRNA tagged with 12 copies of the MS2 stem-loop sequence

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

LINC00673 (LINC00673 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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pLux01

Plasmid

#78281

Purpose

mRFP1 expressed behind an AHL-inducible construct

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

AHL inducible mRFP1

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pLV.PGK.hLHX6

Plasmid

#161828

Purpose

Expresses human LHX6(LIM Homeobox 6) in mammalian cells

Depositor

Daniella Ottosson

Article

Giacomoni et al Cells. 2020 Nov 10;9(11). pii: cells9112

Insert

human LHX6 (LHX6 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

AA 17-23 deletion

Promoter

PGK

Available since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-LckAPEX-P2A-EGFP

Plasmid

#182826

Purpose

Cre-dependent expression of membrane localized APEX

Depositor

Yevgenia Kozorovitskiy

Article

Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

Insert

LckAPEX-P2A-EGFP

Use

AAV

Tags

EGFP reporter (P2A)

Expression

Mutation

Promoter

EF1a

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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hEGFR-mEGFP

Plasmid

#182878

Purpose

Mammalian expression of epidermal growth factor receptor (EGFR) fused to mEGFP

Depositor

Salvatore Chiantia

Article

Tzoneva et al Biomolecules. 2020 May 22;10(5). pii: bi

Insert

hEGFR-mEGFP (EGFR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCS-CG-Lock-Cer-VWF

Plasmid

#124828

Purpose

Expresses multimeric human VWF-ECFP fusion protein with A2-Lock domain The cyan fluorescence protein coding region is inserted between the VWF-D'D3 and A1 domains.

Depositor

Sriram Neelamegham

Article

Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

Insert

VWF with cyan fluorescent protein fusion between D'D3 and A1 domains and Lock A2 domain (VWF Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pTet01

Plasmid

#78283

Purpose

mRFP1 expressed behind an ATc-inducible construct

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

Tet inducible mRFP1

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pET15b-Casp8 delta DED C360A

Plasmid

#11828

Depositor

Guy Salvesen

Article

Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

Insert

Caspase 8 minus Death Effecter Domain catalytic mutant (CASP8 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Minus Death Defecter Domain (aa 1-216), C360A

Promoter

Available since

May 12, 2006

Availability

Academic Institutions and Nonprofits only
pX330_ACTB-i4 sgRNA / hSpCas9

Plasmid

#172828

Purpose

Mammalian expression of a sgRNA targeting the intron 1 position 4 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-H2B.APEX-P2A-EGFP

Plasmid

#182825

Purpose

Cre-dependent expression of H2B-APEX fusion

Depositor

Yevgenia Kozorovitskiy

Article

Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

Insert

H2B.APEX-P2A-EGFP

Use

AAV

Tags

EGFP reporter (P2A)

Expression

Mutation

Promoter

EF1a

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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Bin1 delta N-BAR

Plasmid

#168282

Purpose

Express N-BAR-deleted Bin1 mutant (aa 268-476)

Depositor

Cheng-han Yu

Article

Cao et al Commun Biol. 2020 Mar 13;3(1):117. doi:

Insert

Bin1 (Bin1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

aa 268-476

Promoter

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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Bin1 N-BAR domain

Plasmid

#168280

Purpose

Express N-BAR domain of Bin1 (aa 1-267) in pEGFP-N1 vector

Depositor

Cheng-han Yu

Article

Cao et al Commun Biol. 2020 Mar 13;3(1):117. doi:

Insert

Bin1 (Bin1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-267

Promoter

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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Bin1 delta SH3

Plasmid

#168283

Purpose

Express SH3-deleted Bin1 mutant (aa 1-404) in pEGFP-N1 vector.

Depositor

Cheng-han Yu

Article

Cao et al Commun Biol. 2020 Mar 13;3(1):117. doi:

Insert

Bin1 (Bin1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-404

Promoter

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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gh178

Plasmid

#106828

Purpose

expression of gRNA targeting GYS1

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GYS1 (GYS1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-RIPK3 4KR

Plasmid

#78828

Purpose

To purify GST-tagged recombinant RIPK3 4KR protein

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

K55R, K89R, K363R, K501R

Promoter

tac

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pLux02

Plasmid

#78282

Purpose

mRFP1 expressed behind an AHL-inducible construct

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

AHL inducible mRFP1

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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KN701: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS

Plasmid

#121828

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) (nuclease-dead) fusion for 3 or 4-component MultiSite Gateway Pro assembly.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

x-dCas9(3.7) (open)

Use

Synthetic Biology; Pmagic gateway entry plasmid

Tags

NLS

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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