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R619-M89-303: CMV51p> HCoV-HKU1 S-2P-T4f-3C-His8-Strep2x2

Plasmid

#166014

Purpose

mammalian expression of HCoV-HKU1 soluble spike trimer protein

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

HCoV-HKU1 S (spike)

Use

Tags

3C-His8-Strep2x2

Expression

Mammalian

Mutation

Promoter

CMV51

Available since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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R619-M91-303: CMV51p> HCoV-NL63 S-2P-T4f-3C-His8-Strep2x2

Plasmid

#166017

Purpose

mammalian expression of HCoV-NL63 soluble spike trimer protein

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

HCoV-NL63 S (spike)

Use

Tags

3C-His8-Strep2x2

Expression

Mammalian

Mutation

Promoter

CMV51

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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R619-M90-303: CMV51p> HCoV-229E S-2P-T4f-3C-His8-Strep2x2

Plasmid

#166016

Purpose

mammalian expression of HCoV-229E soluble spike trimer protein

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

HCoV-229E S (spike) (S )

Use

Tags

3C-His8-Strep2x2

Expression

Mammalian

Mutation

Promoter

CMV51

Available since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pET His6 Sumo TEV expression vector with BioBrick polypromoter restriction sites (14-S)

Plasmid

#48313

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6 and Sumo

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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R619-M67-303: CMV51p> SARS-CoV-2 S-RBD(319-541)-His6

Plasmid

#166018

Purpose

mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD)

Depositor

Dominic Esposito

Article

Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

Insert

SARS-CoV-2 RBD (S )

Use

Tags

His6

Expression

Mammalian

Mutation

Promoter

CMV51

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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Gal1/10 His6-MBP TEV Trp S. cerevisiae expression vector (12TRP-C)

Plasmid

#48302

Depositor

Scott Gradia

Type

Empty backbone

Use

Na

Tags

His6 and MBP

Expression

Mutation

Promoter

Available since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only

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Gal1/10 His6-MBP TEV Ade S. cerevisiae expression vector (12ADE-C)

Plasmid

#48299

Depositor

Scott Gradia

Type

Empty backbone

Use

Na

Tags

His6 and MBP

Expression

Mutation

Promoter

Available since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Flag YFP hNKCC1 N-terminus T,S mutations P676C I730C (NT95)

Plasmid

#50913

Purpose

Expresses human NKCC1 with mutated Ser and Thr residues in the N-terminus and mutations at P676C I730C. Contains N-terminal 3xFLAG-YFP tag for expression in mammalian cells

Depositor

Biff Forbush

Article

Monette et al J Biol Chem. 2014 Jan 22.

Insert

human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)

Use

Tags

3x Flag and YFP

Expression

Mammalian

Mutation

S150N S155Q S170Q T177A S183R S193E T203A T205A T…

Promoter

CMV

Available since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 NL

Plasmid

#113442

Purpose

NanoLuc control expression vector

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

NanoLuc

Use

Luciferase

Tags

cmyc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 PA-mCit

Plasmid

#113443

Purpose

ProteinA-mCitrine control expression vector

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

ProteinA-mCitrine

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_CORO1A:1-461

Plasmid

#211111

Purpose

Mammalian expression of full-length human CORO1A. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

CORO1A:M1-K461 (CORO1A Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHTC-HaloTag_VPR:1-96

Plasmid

#211012

Purpose

Mammalian expression of full-length HV1N5 VPR. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

VPR:M1-S96

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNLF1-C_DCAF15:1-600

Plasmid

#210959

Purpose

Mammalian expression of full-length human DCAF15. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DCAF15:M1-L600 (DCAF15 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_TBL1XR1:1-514

Plasmid

#211167

Purpose

Mammalian expression of full-length human TBL1XR1. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

TBL1XR1:M1-K514 (TBL1XR1 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_WDR45:1-360

Plasmid

#211178

Purpose

Mammalian expression of full-length human WDR45. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

WDR45:M1-F360 (WDR45 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_UNG:1-313

Plasmid

#211093

Purpose

Mammalian expression of full-length human UNG. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

UNG:M1-L313 (UNG Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_STRAP:1-350

Plasmid

#211139

Purpose

Mammalian expression of full-length human STRAP. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

STRAP:M1-A350 (STRAP Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_CORO1B:1-489

Plasmid

#211196

Purpose

Mammalian expression of full-length human CORO1B. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

CORO1B:M1-A489 (CORO1B Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_PRPF19:1-504

Plasmid

#211132

Purpose

Mammalian expression of full-length human PRPF19. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

PRPF19:M1-L504 (PRPF19 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHTN-HaloTag_MTA2:1-668

Plasmid

#211016

Purpose

Mammalian expression of full-length human MTA2. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

MTA2:M1-D668 (MTA2 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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