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Showing: 961 - 1000 of 23151 results

  1. pAAV-TNT4-TadA8e-SpRY N aa2-713-InteinN-miR122 TS

    Plasmid
    #209787
    Purpose
    Expresses TadA8e and SpRY cas9N by the specific TNT4 promoter and incorporation of the miR122 target sequences (miR122TS) into the 3’ untranslated region (3’ UTR)
    Depositor
    Yuxuan Guo
    Article
    Yang et al Circulation. 2024 May 28;149(22):1778-17
    Insert
    TNT4, TadA8e, SpRY N, inteinN, miR122 TS
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    TNT4
    Available since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    209787-plasmid-map-sequence-id-416840

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  7. pCMV-T7-nSpRY(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES636)

    Plasmid
    #185491
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage nSpRY(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP
    Depositor
    Raul Perez-Jimenez, Benjamin Kleinstiver
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage nSpRY(D10A)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-eGFP
    Expression
    Mammalian
    Mutation
    D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…
    Promoter
    CMV and T7
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185491-plasmid-map-sequence-id-362675

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  12. pCMV-T7-BPNLS-ABE8e(V106W)-SpRY-BPNLS-P2A-EGFP (CA136)

    Plasmid
    #208291
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e(V106W) A-to-G base editor with SpRY(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e(V106W)-SpRY-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations and V106W in TadA and nSpRY(D10A/…
    Promoter
    CMV and T7
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    208291-plasmid-map-sequence-id-410846

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  13. pRP[CRISPR]-EGFP/Puro-hCas9-U6>(long left)-U6>(long right)

    Plasmid
    #216871
    Purpose
    This plasmid contains hCas9 and two gRNAs that can excise the genomic area around the mouse mdx mutation in dystrophin's exon 23
    Depositor
    Ori Bar-Nur
    Article
    Lenardic et al J Clin Invest. 2024 May 7:e166998. doi:
    Insert
    Cas9, gRNA Long.L, gRNA Long.R (Dmd Mouse)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    mdx mutation in mouse dystrophin
    Promoter
    Available since
    June 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    216871-plasmid-map-sequence-id-435357

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  14. pCMV-T7-nSpRY(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES395)

    Plasmid
    #185489
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage nSpRY(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP
    Depositor
    Raul Perez-Jimenez, Benjamin Kleinstiver
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage nSpRY(H840A)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-eGFP
    Expression
    Mammalian
    Mutation
    H840A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N13…
    Promoter
    CMV and T7
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185489-plasmid-map-sequence-id-362674

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  18. pNDGG002

    Plasmid
    #231308
    Purpose
    Vector part for BEVA; BEVA2.0 Position 2 Sp antibiotic resistance via aadA, AmpR/SpR
    Depositor
    Barney Geddes
    Insert
    BEVA2.0 Position 2 Sp antibiotic resistance via aadA, AmpR/SpR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    231308-plasmid-map-sequence-id-462187

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  19. pMCRi

    Plasmid
    #207524
    Purpose
    Plasmid for CRISPRi; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpR
    Depositor
    Pablo Ivan Nikel
    Article
    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.
    Insert
    xylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpR
    Use
    Pseudomonas vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    207524-plasmid-map-sequence-id-409593

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  20. TACR1-DuET

    Plasmid
    #213385
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    TACR1 (TACR1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213385-plasmid-map-sequence-id-430892

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  21. pNDGG051

    Plasmid
    #231320
    Purpose
    BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-pBBR1-Sp-par-ELT4; GG assembly from pOGG004, pNDGG002, pOGG011, pOGG012, and pOGG014, SpR
    Depositor
    Barney Geddes
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    231320-plasmid-map-sequence-id-463765

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  22. pNDGG070

    Plasmid
    #231328
    Purpose
    BEVA Golden Gate cloning vector; BEVA2.0 Sucrose curable broad host range plasmid . L1-pBBR1-Sp-sacB-ELT4; GG assembly from pOGG004, pNDGG002, pOGG011, pNDGG008, and pOGG014, SpR
    Depositor
    Barney Geddes
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    231328-plasmid-map-sequence-id-466831

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  23. pN3-SP4FL

    Plasmid
    #107719
    Purpose
    Expression plasmid for full-length human Sp4
    Depositor
    Guntram Suske
    Insert
    Sp4 (SP4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 18, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    107719-plasmid-map-sequence-id-207621

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  24. pCLIPf-NK1R Control Plasmid

    Plasmid
    #101125
    Purpose
    Expression of CLIP-tagged Neurokinin-1 Receptor in Mammalian cells
    Depositor
    New England Biolabs, Ana Egana
    Insert
    Neurokinin-1 Receptor, Truncated (TACR1 Human)
    Use
    Tags
    10xHis, CLIP-tag (CLIPf), and FLAG Epitope
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101125-plasmid-map-sequence-id-194114

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  31. pEditC

    Plasmid
    #207532
    Purpose
    Plasmid for cytidine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→CBE:SpnCas9, PEM7→non-specific sgRNA; SmR/SpR
    Depositor
    Pablo Ivan Nikel
    Article
    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.
    Insert
    xylS (cured of BsaI-sites), Pm→CBE:SpnCas9, PEM7→non-specific sgRNA
    Use
    CRISPR; Pseudomonas vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    207532-plasmid-map-sequence-id-409609

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  32. pEditA-RY

    Plasmid
    #207533
    Purpose
    Plasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ABE:SpRY, PEM7→non-specific sgRNA; SmR/SpR
    Depositor
    Pablo Ivan Nikel
    Article
    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.
    Insert
    xylS (cured of BsaI-sites), Pm→ABE:SpRY, PEM7→non-specific sgRNA
    Use
    CRISPR; Pseudomonas vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    207533-plasmid-map-sequence-id-409612

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  33. pEditC-RY

    Plasmid
    #207534
    Purpose
    Plasmid for cytidine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→CBE:SpRY, PEM7→non-specific sgRNA; SmR/SpR
    Depositor
    Pablo Ivan Nikel
    Article
    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.
    Insert
    xylS (cured of BsaI-sites), Pm→CBE:SpRY, PEM7→non-specific sgRNA
    Use
    CRISPR; Pseudomonas vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    207534-plasmid-map-sequence-id-409613

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  34. pS44i8GH-2

    Plasmid
    #207526
    Purpose
    Conditionally-replicating in Pseudomonas vector, high-copy-number; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b→msfGFP; SmR/SpR
    Depositor
    Pablo Ivan Nikel
    Article
    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.
    Insert
    oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b→msfGFP
    Use
    CRISPR; Pseudomonas vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    207526-plasmid-map-sequence-id-409598

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  35. pS44i8GM

    Plasmid
    #207527
    Purpose
    Conditionally-replicating in Pseudomonas vector, medium-copy-number; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP; SmR/SpR
    Depositor
    Pablo Ivan Nikel
    Article
    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.
    Insert
    oriV(pRO1600/ColE1), xylS, Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP
    Use
    CRISPR; Pseudomonas vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    207527-plasmid-map-sequence-id-409603

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  36. pEditA

    Plasmid
    #207531
    Purpose
    Plasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ ABE:SpnCas9, PEM7→non-specific sgRNA; SmR/SpR
    Depositor
    Pablo Ivan Nikel
    Article
    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.
    Insert
    Plasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ ABE:SpnCas9, PEM7→non-specific sgRNA
    Use
    CRISPR; Pseudomonas vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    207531-plasmid-map-sequence-id-409606

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  37. pS44i8GH-1

    Plasmid
    #207525
    Purpose
    Conditionally-replicating in Pseudomonas vector, high-copy-number; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP; SmR/SpR
    Depositor
    Pablo Ivan Nikel
    Article
    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.
    Insert
    oriV(pRO1600/ColE1), xylS, Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP;
    Use
    CRISPR; Pseudomonas vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    207525-plasmid-map-sequence-id-409596

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  38. TACR1-Tango

    Plasmid
    #66514
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    TACR1 (TACR1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  39. pYPQ266E

    Plasmid
    #161521
    Purpose
    Gateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 PmCDA1 mediated C-T base editing
    Depositor
    Yiping Qi
    Article
    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41
    Insert
    zSpRYCas9(D10A)-PmCDA1-UGI
    Use
    CRISPR; Gateway compatible zsprycas9(d10a)-pmcda1…
    Tags
    3X FLAG, NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    161521-plasmid-map-sequence-id-325638

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  40. pYPQ262B-ABE8e

    Plasmid
    #161524
    Purpose
    Gateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 ABE8e mediated A-G base editing
    Depositor
    Yiping Qi
    Article
    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41
    Insert
    TadA8e-zSpRYCas9(D10A)
    Use
    CRISPR; Gateway compatible tada8e-zsprycas9(d10a)…
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    161524-plasmid-map-sequence-id-325557

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Showing: 961 - 1000 of 23151 results