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Plasmid#192438PurposeEncodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant targeted to lysosome membrane.DepositorInsertLyso-mCherry-Rheb(C181S) (RHEB Human)
UseTagsFull-length LAMP1 and mCherryExpressionMammalianMutationRheb Cys 181 mutated to Ser; abolishes C-terminal…PromoterCMVAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-Rheb(C181S)
Plasmid#192437PurposeEncodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant.DepositorInsertmCherry-Rheb(C181S) (RHEB Human)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationRheb Cys 181 mutated to Ser; abolishes C-terminal…PromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-HEPN
Plasmid#75296PurposeExpresses Sacsin HEPN domain (4441-4579) in fusion with GST (bacterial)DepositorInsertHuman Sacsin 4441-4579
UseTagsGST-TagExpressionBacterialMutationPromoterAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
SLF045: AAV-hSyn-eTsChR-GFP
Plasmid#112280PurposeAAV based broad expression of eTsChR in neuronsDepositorInserteTsChR
UseAAVTagsGFPExpressionMammalianMutationPromoterhSynAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
SuExp-hPLD4
Plasmid#201244Purposeexpress full length human PLD4 proteinDepositorInsertPLD4 (PLD4 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACEC3GH (Hyg)
Plasmid#110084PurposeReplicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.DepositorTypeEmpty backboneUseTags3C cleavage site - GFP - His10 TagExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1A-DIO-mRuby2
Plasmid#126215PurposeLabeling of cre-expressing neurons (red fluorescence)DepositorInsertmRuby2
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1AAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUB-sSypHer (RbcS-SypHer)
Plasmid#84732PurposeExpresses SypHer in plant cells-biosensor for pH targeted to chloroplasts stomaDepositorInsertsSypHer
UseTagsRbcS targeting sequenceExpressionPlantMutationPromoterubiqutin- 10 gene promoter (pUBQ10) of ArabidopsisAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSBbi_RP_2XMTS-MicroID2-FLAG
Plasmid#188524PurposeEncodes the biotin proximity labeling enzyme MicroID2 with a 2X mitochondrial targeting sequence (MTS) for expression and stable integration into mammalian cellsDepositorInsert2XMTS-MicroID2
UseTransposon mediated gene integrationTagsFLAGExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
β3-integrin-CFP
Plasmid#26652DepositorInsertβ3-integrin (ITGB3 Human)
UseTagsCFPExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-150
Plasmid#118564PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertTagBFP2
UseMinitn7 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNAIIIB cdc42 WT
Plasmid#61635Purposemammalian expression of cdc42 wtDepositorInsertcdc42 (CDC42 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Flag-hsDicer (D1320A/D1709A)
Plasmid#41585DepositorInserthsDicer (DICER1 Human)
UseTagsFlagExpressionMammalianMutationD1320A/D1709APromoterCMVAvailable sinceFeb. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
ePPE-V223A
Plasmid#205243PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertSV40_NLS-nCas9-NC-MLV-SV40_NLS
UseCRISPRTagsExpressionPlantMutationDetailed in manuscriptPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
YFP Rab4DN
Plasmid#99019PurposeLocalization and visualization of the dominant negative form of Rab4DepositorInsertRab4 DN (Rab4 Synthetic, Fly)
UseTagsYFPExpressionMammalianMutationS22N Dominant negativePromoterCMVAvailable sinceJune 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Syn-PSD95.FingR-eGFP
Plasmid#126212PurposeLabeling of excitatory synapses (green fluorescence) in dividing cellsDepositorInsertPSD95.FingR-eGFP
UseRetroviralTagsExpressionMammalianMutationPromoterSynapsinAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-161
Plasmid#118573PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmTurquoise2
UseMinitn7 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRetroSuper-GFP Smad2
Plasmid#15722DepositorInsertSmad2 shRNA (SMAD2 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-LacR-TopBP1
Plasmid#31313DepositorInsertTopBP1 (TOPBP1 Human)
UseTagsFLAG and LacRExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P29
Plasmid#120040PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Strep-IIDepositorInsertStrep-II
UsePcr templateTagsStrep-IIExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only