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Plasmid#70052Purpose5' Entry vector containing the zebrafish mfap4 promoter. Used with Gateway Recombination to generate transgenic constructs exhibiting macrophage-restricted expression of transgenes.DepositorInsertmfap4 promoter
UseGateway 5' elementTagsExpressionMutationPromoterAvailable sinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE_ENTR_PTCH1-mTurquoise2_R4-R3
Plasmid#113864PurposeSingle pathway reporter; hedgehog promoter-driven expression of mTurquoise2; MultiSite Gateway entry vector.DepositorInsertsPTCH1 (hedgehog dependent promoter)
mTurquoise2
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pME-Lifeact
Plasmid#109545PurposeMultisite gateway vector for 5' tagging with the actin binding motif LifeactDepositorInsertactin binding motif Lifeact
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSC6
Plasmid#104780PurposepSC218UG-At Ubiquitin10:Gateway expression cassette binary vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC22A1
Plasmid#132034PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC22A1 (SLC22A1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
YFC43
Plasmid#89488Purposegateway-based Bimolecular Fluorescence Complementation (BiFC) binary vector containing C terminal fragment of YFPDepositorTypeEmpty backboneUseTagsYFP-C (aa 154-240)ExpressionPlantMutationPromoter2x35SAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
YFN43
Plasmid#89487Purposegateway-based Bimolecular Fluorescence Complementation (BiFC) binary vector containing N terminal fragment of YFPDepositorTypeEmpty backboneUseTagsYFP-N (aa 1-154)ExpressionPlantMutationPromoter2x35SAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3E-mScarlet
Plasmid#140875PurposeMultisite Gateway middle entry vector for fusing mScarlet to the 3' end of an ORF.DepositorInsertmScarlet
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-DEST(f)
Plasmid#190226PurposeGateway destination vector, empty AAV vector backboneDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-LC3C WT
Plasmid#123095PurposeExpresses 3xFLAG-LC3C wild-type in mammalian cells.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDestattB_ubi:EGFP (pCM272)
Plasmid#68339PurposeMultisite Gateway destination vector for phiC31-mediated transgenesis in zebrafish, harbors ubiquitin promoter (ubi)-driven EGFP.DepositorInsertEGFP
UseZebrafish transgenesisTagsExpressionMutationPromoterubiAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pALS099 (pGEX-2TP-cTevHis-DEST)
Plasmid#139787PurposeGateway destination vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHisDepositorTypeEmpty backboneUseTags6xHis (cleavable by TEV protease) and GST (cleava…ExpressionBacterialMutationPromoterAvailable sinceApril 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3E-EGFP
Plasmid#140877Purpose126573: Multisite Gateway middle entry vector for fusing mScarlet to the 3' end of an ORF.DepositorInsertEGFP
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
p5E-4xnrUAS
Plasmid#87120PurposeMultisite gateway entry clone containing 4 non-repetitive UAS (upstream activation sequence) elementsDepositorInsert4xnrUAS (non repetitive)
UseGateway vectorTagsExpressionMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDestattB/CY (pCM327)
Plasmid#68341PurposeMultisite Gateway destination vector to create transgenes for phiC31-mediated transgenesis in zebrafish, contains an attB site and alpha-cystallin:YFP (CY) transgenesis marker in backbone.DepositorTypeEmpty backboneUseZebrafish transgenesisTagsExpressionMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pME-mScarlet-STOP
Plasmid#140876PurposeMultisite Gateway middle entry vector for expression of untagged mScarlet.DepositorInsertmScarlet
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
tetO-Myocd
Plasmid#46033DepositorInsertMyocd (Myocd Mouse)
UseLentiviralTagsExpressionMammalianMutationPromotertetOAvailable sinceJuly 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMuLE_ENTR_CBF-GLuc_L3-L2
Plasmid#113868PurposeSingle pathway reporter; notch promoter-driven expression of secreted Gaussia luciferase; MultiSite Gateway entry vector.DepositorInsertsCBF (notch dependent promoter)
GLuc
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJW4.16
Plasmid#62871PurposeBacterial expression of MBP-MBK-2DepositorInsertMBP-MBK-2
UseTagsMBPExpressionBacterialMutationPromoterAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC90-pmax-DEST
Plasmid#48222PurposeGateway Destination vector derived from Clontech's pmax (CAGGS) expression vectorDepositorTypeEmpty backboneUseCRISPR; Gateway destinationTagsExpressionMammalianMutationPromoterCAGGSAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only