-
Plasmid#28105DepositorInsertWIP1 (PPM1D Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceApril 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
CMV-SNAP-hCB1_IRES_EGFP
Plasmid#223505PurposeExpression of CB1 N-terminally fused SNAP-tag, with targeting sequence to boost plasma membrane expression. Coupled with EGFP transfection markerDepositorInsertSNAP-tag-CNR1 and EGFP (CNR1 Synthetic, Human)
UseTagsSNAP-tagExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
nNOS/NOS1 scFv [L121/133]
Plasmid#206759PurposeMammalian Expression of nNOS/NOS1 scFV. Derived from hybridoma L121/133 scFv.DepositorInsertnNOS/NOS1 (Homo sapiens) recombinant scFV (NOS1 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10.9RT2-Pct5.1-crRNA(cadR)-RT(ΔcadR)
Plasmid#191647PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cadR-targeting spacer) cadR-deleting repair template,used for cadR deletionDepositorInsertCymR
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2-dADAM17 (#1)
Plasmid#177259PurposeA knockout vector for the dog Adam17DepositorInsertA gRNA targeting the dog Adam17 gene and the cDNA of CRISPR-Cas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cys-60nmSAH-SPYcatcher
Plasmid#214840PurposeThis plasmid encodes a protein (Cys-60nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertCys-60nmSAH-SPYcatcher
UseTagsHis-TEV-SUMO-ExpressionBacterialMutationPromoterT5Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKH118_keima-YIPF4
Plasmid#214971PurposeGolgiphagy flux reporter keima-YIPF4DepositorInsertkeima-YIPF4 (YIPF4 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD-mhYFP
Plasmid#186532PurposeMonomeric hyperfolder YFP fluorescent protein: bacterial expression plasmidDepositorInsertMonomeric hyperfolder YFP
UseTags6xHisExpressionBacterialMutationhfYFP-S147P/L195M/V206KPromoteraraBAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF5B-FRT-cilia-APEX
Plasmid#73186PurposeExpresses cilia-APEX in mammalian cellsDepositorInsertcilia-APEX (Nphp3 Mouse, Glycine max)
UseTagsinternal GFP tagExpressionMammalianMutationPromoterEF1alphaAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
MLV Gag-YFP
Plasmid#1813DepositorInsertGag (gag MuLV)
UseTagsYFPExpressionMammalianMutationPromoterAvailable sinceMay 19, 2005AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC3A2_STOP
Plasmid#161379PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC3A2 (SLC3A2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFP-Nup88
Plasmid#64283PurposeGFP-tagged mammalian expression of full-length Nup88DepositorInsertNup88 (NUP88 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE5
Plasmid#90339PurposeSTAT3 - Stat3 homodimer gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterSTAT3Available sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-SpCas9
Plasmid#104588PurposeAAV vector expressing Myc-tagged SpCas9 under the EFS promoterDepositorInsertStreptococcus pyogenes Cas9
UseAAV and CRISPRTagsMycExpressionMammalianMutationPromoterEFSAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZR040_Lenti-U6-sgCtrl-MS2-PP7-hPGK-PCP-p65-HSF1-Puro
Plasmid#180266PurposeLentiviral expression vector for CRISPRa-SAM with non-targeting control sgRNADepositorInsertNo-Target Control sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF1894
Plasmid#144200PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertAHRR (AHRR Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
DR274
Plasmid#42250Purposeguide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcriptionDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR; Zebrafish expressionTagsExpressionWormMutationPromoterT7Available sinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
BB3cK_pGAP_23*_pLAT1_Cas9
Plasmid#104908PurposehCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418DepositorInsertsHH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMRX-mSTING-EGFP
Plasmid#214148PurposeRetroviral transduction of EGFP-tagged STINGDepositorInsertSTING (Sting1 Mouse)
UseRetroviralTagsEGFPExpressionMutationPromoterAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ERBB4
Plasmid#23875DepositorInsertERBB4 (ERBB4 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
TFORF2195
Plasmid#142181PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMLXIPL (MLXIPL Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Kir2.1Mut-T2A-tdTomato
Plasmid#60644PurposeExpresses Kir2.1Mut-T2A-tdTomatoDepositorInsertKcnj2 (Kcnj2 Mouse)
UseTagsmycExpressionMammalianMutationGYG to AAA (aa144-146)PromoterAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-FLAG-GFP-OSBP
Plasmid#134659PurposeFlag and GFP-tagged OSBPDepositorInsertOSBP (OSBP Human)
UseLentiviralTagsFLAG and GFPExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pB1H2 UV5 Zif268-5ala (2-hybrid bait plasmid)
Plasmid#128165PurposeUV5 promoter drives expression of Zif268-5alanine peptide fusion. Pair with pGHUC w-ERBIN as a negative control (doesn't turn on HIS3/GFP).DepositorInsert10 amino acid glycine/serine linker + 5alanines
UseSynthetic BiologyTagsFLAG and zif268ExpressionBacterialMutationPromoterUV5Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSET-6xTR-TUBE
Plasmid#110313PurposeE. coli expression and purification of 6xTR-TUBEDepositorInsert6xTR-TUBE (UBQLN1 Human)
UseTagsN-terminal His6-T7 tagExpressionBacterialMutationAll Arg residues in the UBA domain are mutated to…PromoterT7Available sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-GAL4-iLight-VP16
Plasmid#170274PurposeNLS-Gal4(1-65)-iLight(human codon optimized)-VP16 construct expression in mammalian cells. pAAV vector.DepositorInsertNLS-Gal4(1-65)-iLight(human codon optimized)-VP16
UseAAVTagsExpressionMutationSee CommentsPromoterCaMKIIAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-dTAG-Puro
Plasmid#207727PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Puro cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pENN.AAV.CamKII.GCaMP6f.WPRE.SV40
Plasmid#100834PurposeAAV expression of ultrasensitive protein calcium sensor from CamKII promoterDepositorHas ServiceAAV1, AAV5, and AAV9InsertGCaMP6f
UseAAVTagsExpressionMammalianMutationGCaMP3-T302L R303P A317E D380Y T381R S383T R392GPromoterCamKIIAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLJC6-2XFLAG-TMEM192
Plasmid#104435PurposeExpresses 2XFLAG-TMEM192 in mammalian cellsDepositorInsertTMEM-192 (TMEM192 Human)
UseLentiviralTags2XFLAGExpressionMammalianMutationPromoterUbiquitinAvailable sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
ABE8.8-m
Plasmid#136294Purposeexpresses ABE8.8-m in mammalian cellsDepositorInsertABE8.8-m
UseTagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only